Antioxidant and Anticancer Effects of Malva verticillata Methanolic Extract

Sonuç: Bu çalışma ile birrlikte Kuzey Kıbrıs'ta yetiştirilen M. verticillata bitkisinin metanolik ekstraktının önemli antioksidan ve antikanser aktivitesine sahip olduğu gösterilmiştir.

📌 3 Key Takeaways

🎯

Objective & Hypothesis:Abstract Objectives: Malva verticillata (M. verticillata), growing in North Cyprus, is an edible plant known as "mallow" in public.

📊

Key Findings & Data:Besides, 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay was used to investigate the anticancer potential of M. verticillata methanolic extract on MCF-7 cells.

💡

Conclusion & Impact:Sonuç: Bu çalışma ile birrlikte Kuzey Kıbrıs'ta yetiştirilen M. verticillata bitkisinin metanolik ekstraktının önemli antioksidan ve antikanser aktivitesine sahip olduğu gösterilmiştir.

🔬Design:Review & Systematic Survey

📍Scope:Comprehensive Sample

⚙️Variables:Antioksidan · Anticancer · Breast cancer (MCF-7)

Abstract

Abstract Objectives: Malva verticillata (M. verticillata), growing in North Cyprus, is an edible plant known as "mallow" in public. Current literature does not contain any data about the anticancer and antioxidant activities of the M. verticillata plant grows in Northern Cyprus. Materials and Methods: In this study, M. verticillata methanolic extract was used to investigate the in vitro antioxidant and anticancer effects of the M. verticillata plant. The antioxidant potential of the Malva extract was determined using the α-diphenyl-p-picrylhydrazyl (DPPH) free radical scavenging method, the total phenolic content (TPC) test, and the total flavonoid content (TFC) test. Besides, 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay was used to investigate the anticancer potential of M. verticillata methanolic extract on MCF-7 cells. Results: The highest antioxidant activity of M. verticillata methanolic extract was found to be 69.35 ± 3.3% at 70 mg/ml. TFC was calculated as 502 ± 1.8 μg/ml extract and TPC 499 ± 7.5 μg/mg extract at 70 mg/ml. When the anticancer activity was evaluated, it was observed that the MCF-7 cell proliferation was significantly inhibited with the varying extract concentrations of M. verticillata. Conclusion: With this study, it has been shown that the methanolic extract of M. verticillata plant grown in Northern Cyprus has significant antioxidant and anticancer activity. Key words: Antioxidant, Anticancer, Breast cancer (MCF-7), Malva verticillata. Öz Amaç: Kuzey Kıbrıs'ta yetişen Malva verticillata (M. verticillata), halk arasında "ebegümeci" olarak da isimlendirilen ve yenilebilen bir bitki türüdür. Güncel literatür, Kuzey Kıbrıs'ta yetişen M. verticillata bitkisinin antikanser ve antioksidan özellikleri hakkında herhangi bir veri içermemektedir. Gereç ve Yöntemler: Bu çalışmada M. verticillata bitkisinin in vitro antioksidan ve antikanser potansiyelini araştırmak için M. verticillata metanolik ekstraktı kullanıldı. Ekstraktın antioksidan potansiyeli, α-difenil-p-pikrilhidrazil (DPPH) serbest radikal süpürme yöntemi, toplam fenolik içerik (TPC) testi ve toplam flavonoid içerik (TFC) testi kullanılarak belirlendi. Ayrıca, M. verticillata metanolik ekstraktın MCF-7 meme kanseri hücreleri üzerindeki antikanser aktivitesini değerlendirmek üzere MTT yöntemi (3-(4,5- dimetiltiyazol-2-il)-2,5-difeniltetrazolyum bromid) kullanıldı. Bulgular: M. verticillata metanolik ekstraktın en yüksek antioksidan aktivitesi 70 mg/ml'de %69,35 ± %3,3 olarak bulundu. TPC, 70 mg/ml'de 499 ± 7.5 μg/mg ekstre ve TFC 502 ± 1.8 μg/ml ekstre olarak hesaplandı. Antikanser aktivitesi değerlendirildiği zaman, M. verticillata'nın değişen ekstre konsantrasyonlarının, MCF-7 meme kanseri hücrelerinin çoğalmasını önemli ölçüde inhibe ettiği gözlemlenmiştir. Sonuç: Bu çalışma ile birrlikte Kuzey Kıbrıs'ta yetiştirilen M. verticillata bitkisinin metanolik ekstraktının önemli antioksidan ve antikanser aktivitesine sahip olduğu gösterilmiştir.

Keywords

Antioksidan Anticancer Breast cancer (MCF-7)

🔖

Cite This Article

GENCALP, D., KERKÜKLÜ, N. R., BUDA, Ö., BİNER, G., TURGAL, E., POLAT, D., İRDEM, F., KASAPOĞLU, N., ALTUNDAG, E. M. (2020). Antioxidant and Anticancer Effects of Malva verticillata Methanolic Extract. Current Perspectives on Medicinal and Aromatic Plants, Cilt: 3 - Sayı: 2, 113–120. https://ajindex.com/articles/748982

📄 Full Text PDF Viewer

Open in New Tab ↗

↗New Tab

📄

PDF görüntüleyici hazırlanıyor...

References (10)

References Alara, Oluwaseun Ruth, Nour Hamid Abdurahman, and Olusegun Abayomi Olalere. 2018a. “Ethanolic Extraction of Bioactive Compounds from Vernonia Amygdalina Leaf Using Response Surface Methodology as an Optimization Tool.” Journal of Food Measurement and Characterization 12(2):1107–22.
Alara, Oluwaseun Ruth, Nour Hamid Abdurahman, and Olusegun Abayomi Olalere. 2018b. “Optimization of Microwave-Assisted Extraction of Flavonoids and Antioxidants from Vernonia Amygdalina Leaf Using Response Surface Methodology.” Food and Bioproducts Processing 107:36–48.
Anon. n.d. “Treatment of Breast Cancer - PubMed.” Retrieved August 4, 2020 (https://pubmed.ncbi.nlm.nih.gov/20521754/).
Ashok, Ad, J. Ravivarman, and K. Kayalvizhi. 2020. “Underutilized Leafy Vegetables of India and Their Pharmaceutical Value to Provoke Human Immune System.” ~ 1319 ~ Journal of Pharmacognosy and Phytochemistry 9(3). Bao, Liming, Xiaohua Bao, Peng Li, Xiulan Wang, and Wuliji Ao. 2018. “Chemical Profiling of Malva Verticillata L. by UPLC-Q-TOF-MSE and Their Antioxidant Activity in Vitro.” Journal of Pharmaceutical and Biomedical Analysis 150:420–26.
Benetou, Vassiliki, Philippos Orfanos, Pagona Lagiou, Dimitrios Trichopoulos, Paolo Boffetta, and Antonia Trichopoulou. 2008. “Vegetables and Fruits in Relation to Cancer Risk: Evidence from the Greek EPIC Cohort Study.” Cancer Epidemiology Biomarkers and Prevention 17(2):387–92.
Bray, Freddie, Jacques Ferlay, Isabelle Soerjomataram, Rebecca L. Siegel, Lindsey A. Torre, and Ahmedin Jemal. 2018. “Global Cancer Statistics 2018: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries.” CA: A Cancer Journal for Clinicians 68(6):394–424.
Carocho, Márcio, and Isabel Ferreira. 2013. “The Role of Phenolic Compounds in the Fight against Cancer – A Review.” Anti-Cancer Agents in Medicinal Chemistry 13(8):1236–58.
Cassileth, Barrie R., and Christopher C. Chapman. 1996. “Alternative and Complementary Cancer Therapies.” Cancer 77(6):1026–34.
Chahar, Maheep K., Neelu Sharma, Mahabeer P. Dobhal, and Yogesh C. Joshi. 2011. “Flavonoids: A Versatile Source of Anticancer Drugs.” Pharmacognosy Reviews 5(9):1–12.
Choukri Beghdad, Mohammed, Chahid Benammar, Fatima Bensalah, Fatima-Zohra Sabri, Meriem Belarbi, and Farid Chemat. 2014. “Antioxidant Activity, Phenolic and Flavonoid Content in Leaves, Flowers, Stems and Seeds of Mallow (Malva Sylvestris L.) from North Western of Algeria.” African Journal of Biotechnology 13(3):486–91. De