SavedAdd your journal

Journals / Biyolojik Çeşitlilik ve Koruma / 2013 / Cilt: 6 - Sayı: 3

A role of catalase CAT in detoxification of reactive oxygen species ROS in tomato Lycopersicum esculentum contaminated with manganese Mn2+

Mangan Mn2+ ile kontamine olmuş domates Lycopersicum esculentum bitkisinde reaktif oksijen türlerinin ROS detoksifikasyonunda katalazın CAT rolü

Semra SOYDAM-AYDIN
İlker BÜYÜK
B. Pelin ÇELİKKOL
Sümer ARAS
Pages
140–145
Publication Date
2013-12-15
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Also, antioxidant responses to Mn2+ contamination could be reflected as changes in CAT enzyme activity in tomato plants

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In the current study, tomato Lycopersicum esculentum L. plants were exposed to various concentrations of Mn2+ 80, 160, 320, 640 and 1280 μM cation.
📊
Key Findings & Data:In the first part, in order to obtain evidence that plants were in stress, following the Mn2+ treatments, lipid peroxidation were determined.
💡
Conclusion & Impact:Also, antioxidant responses to Mn2+ contamination could be reflected as changes in CAT enzyme activity in tomato plants
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:domates Lycopersicum esculentum · lipitper oksidasyonu · katalaz CAT

Abstract

In the current study, tomato Lycopersicum esculentum L. plants were exposed to various concentrations of Mn2+ 80, 160, 320, 640 and 1280 μM cation. In the first part, in order to obtain evidence that plants were in stress, following the Mn2+ treatments, lipid peroxidation were determined. In the second part, to gain an idea about regulation of the CAT gene product, enzyme activities were also recorded in Mn2+ treated tomato plants. The maximum lipid peroxidation level was determined in plants which were exposed to 1280 μM concentration of Mn2+ contamination. Changes in lipid peroxidation and CAT enzyme activities in tomato plants exposed to different concentration of Mn2+ contamination revealed no positive correlation. The current study revealed that, ROS induced lipid peroxidation level has depended on concentration of Mn2+ contamination. Also, antioxidant responses to Mn2+ contamination could be reflected as changes in CAT enzyme activity in tomato plants

Özet

Bu çalışmada, domates bitkileri Lycopersicum esculentum L. çeşitli konsantrasyonlardaki 80, 160, 320, 640 ve 1280 μM Mn2+ katyonuna maruz bırakılmıştır. Çalışmanın ilk bölümünde Mn2+ stresini takiben bitkilerin stres etkisine gitmiş olduklarını göstermek için lipit peroksidasyonu düzeyleri belirlenmiştir. İkinci bölümde CAT gen ürününün regülasyonu değerlendirmek için Mn2+ stresli domates bitkilerinde enzim aktivitesi ölçülmüştür. En yüksek lipit peroksidasyonu 1280 μM konsantrasyonda Mn2+ stresine maruz kalan bitkilerde gözlenmiştir. Farklı konsantrasyonlardaki Mnarasında pozitif bir korelasyona rastlanmamıştır. Bu çalışma ile ROS ile tetiklenen lipit peroksidasyonu seviyesinin Mn2+ stresinin konsantrasyonuna bağlı olduğu belirlenmiştir. Aynı zamanda Mn2+ stresine karşı oluşan antioksidan cevapların domates bitkisinde CAT enzim aktivitesinin değişimi olarak yansıyabildiği gözlenmiştir

🔖

Cite This Article

SOYDAM-AYDIN, S., BÜYÜK, İ., ÇELİKKOL, B. P., ARAS, S. (2013). A role of catalase CAT in detoxification of reactive oxygen species ROS in tomato Lycopersicum esculentum contaminated with manganese Mn2+. Biyolojik Çeşitlilik ve Koruma, Cilt: 6 - Sayı: 3, 140–145. https://ajindex.com/articles/744756

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (12)

  1. Aebi, H. 1984. Catalase in vitro.Methods Enzymol.105. 121-126.
  2. Alscher, R.G., Donahue, J.I., Cramer, C.L. 1997. Reactive oxygen species and antioxidants: relationships in green cells. Physiol. Plant. 100. 224-233.
  3. Aras, S., Beyaztaş, T., Cansaran-Duman, D., Gökçe, E. 2012. Evaluation of genotoxicity of Pseudeverniafurfuracea (L.)Zopf by RAPD Analysis.Genetics and Molecular Research. 10 (4): 3760 – 3770. Behera, B., Das, A. B., Mittra, B. 2009. Changes in proteinsandantioxidativeenzymes in treemangroves Bruguieraparvifloraand Bruguieragymnorrhizaunder high NaCl stress. Biological DiversityandConservation. 2/2: 71-77.
  4. Ben Ammar, W., Nouairi, I., Tray, B., Zarrouk, M., Jemal, F., Ghorbel, M.H. 2005. Cadmium effects on mineral nutrition and lipid contents in tomato leaves (in french). J. Soc. Biol. 199. 157-163.
  5. Boojar, M.M.A., Goodarzi, F. 2008. Comparative evaluation of oxidative stress status and manganese availability in plants growing on manganese mine.Ecotox. Environ. Safety. 71. 692-699.
  6. Bueno, P., Piqueras, A. 2002. Effect of transition metals on stress, lipid peroxidation and antioxidant enzyme activities in tobacco cell cultures.Plant Growth Regulation.36/2.161-167.
  7. Cansaran-Duman, D., Atakol, O., Aras, S., Atasoy, İ. 2012.Accumulation of trace elements in Pseudeverniafurfuracea (L.)Zopf.transplanted to a polluted site in Ankara and assesment of air pollution genotoxicity by molecular markers. Ekoloji.21. 85.1-14.
  8. Cansaran-Duman, D., Altunkaynak, E., Aras, S. 2013. Heavy metal accumulation and genotoxicity indicator capacity of the lichen species, Ramalinapollinaria collected from around the iron-steel factory in Karabük, Turkey. Turkish Journal of Botany.Accepted for publication. Chahid, K., Laglaoui, A., Zantar, S., Ennabili, A. 2013. Effect of three insecticides on tomato (Solanumlycopersicum) seedling germination and early plants growth.Biological Diversity and Conservation.6/1.57-61.
  9. Cheng, S. 2003. Effects of heavy metals on plants and resistance mechanisms. A state-of-the-art report with special reference to literature published in Chinese journals. Environ SciPollut Res Int.10/4. 256-64.
  10. Çolak, G., Keser, Ö., Caner, N. 2011.
  11. The effects of NaCl, Na2SO4 and Na2CO3 type salt stress some macromorphological parameters about Lycopersiconesculentum (tomato) and Raphanussativus(radish) which in first seedling growth period. Biological Diversity and Conservation. 4/2. 29-48.
  12. DalCorso, G., Farinati, S., Maistri, S., Furini, A. 2008. How plants cope wi