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Journals / Uludağ Üniversitesi Tıp Fakültesi Dergisi / 2022 / Cilt: 48 - Sayı: 2

Effects of UTP on Viability in RAW 264.7 Cell Line

UTP’nin Raw 264.7 Hücre Hattında Canlılık Üzerine Etkileri

Erkan ERMİŞ
Zeynep CANSEV
Onur ETGÜ
Diğdem YÖYEN ERMİŞ
Barbaros ORAL
Pages
255–260
Publication Date
2022-09-15
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

We found that while there was no difference among groups at 24 or 48 hours following UTP administration, cell viability was enhanced by 27,7% (p

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Macrophages are important cells of immune system and their deficiency or dysfunction lead to severe diseases.
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Key Findings & Data:Uridine-5’-triphosphate, a pyrimidine nucleotide, is known to behave as a signaling molecule for macrophages.
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Conclusion & Impact:We found that while there was no difference among groups at 24 or 48 hours following UTP administration, cell viability was enhanced by 27,7% (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:UTP · Raw 264.7 · makrofaj

Abstract

Macrophages are important cells of immune system and their deficiency or dysfunction lead to severe diseases. Uridine-5’-triphosphate, a pyrimidine nucleotide, is known to behave as a signaling molecule for macrophages. However, the effect of UTP on macrophage viability or proliferation is yet to be researched. Therefore, we investigated the effects of different concentrations (1, 10 and 100 μM) of UTP on RAW 264.7 mouse macrophage cell viability at different time points using MTT [(3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide)] assay. We found that while there was no difference among groups at 24 or 48 hours following UTP administration, cell viability was enhanced by 27,7% (p

Özet

Makrofajlar immün sistemin önemli hücreleridir ve eksiklikleri ya da disfonksiyonları vücutta ciddi hastalıklara yol açar. Bir pirimidin nükleotidi olan üridin-5’-trifosfat’ın (UTP) makrofajlar için bir sinyal molekülü gibi davrandığı bilinmektedir. Ancak UTP’nin makrofaj canlılığı ya da proliferasyonu üzerine etkileri henüz araştırılmamıştır. Dolayısıyla bu çalışmada farklı konsantrasyonlarda (1, 10 ve 100 μM) uygulanan UTP’nin RAW 264.7 fare makrofaj hücrelerinin canlılığı üzerine etkileri farklı zaman noktalarında MTT [3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromür] testi kullanılmak suretiyle incelenmiştir. UTP uygulandıktan 24 ya da 48 saat sonra hücre canlılığında kontrole göre herhangi bir fark gözlenmezken, 10 μM konsantrasyonda uygulanan UTP’nin 72 saat sonra hücre canlılığını kontrole göre %27,7 oranında (p

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Cite This Article

ERMİŞ, E., CANSEV, Z., ETGÜ, O., ERMİŞ, D. Y., ORAL, B. (2022). Effects of UTP on Viability in RAW 264.7 Cell Line. Uludağ Üniversitesi Tıp Fakültesi Dergisi, Cilt: 48 - Sayı: 2, 255–260. https://ajindex.com/articles/709158

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