SavedAdd your journal

Journals / İstanbul Journal of Pharmacy / 2022 / Cilt: 52 - Sayı: 1

Chemical constituent of Isochrysis galbana microalgae extract and its cytotoxic activities on leukemic cell lines

Belkis ATASEVER ARSLAN
Kaan YILANCIOĞLU
Seda KUŞOĞLU GÜLTEKİN
İrem Gülfem ALBAYRAK
Pages
64–68
Publication Date
2022-04-28
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

To determine the effects of Isochrysis galbana extract, an MTT cytotoxicity assay was performed.Results: According to the results of the experiments, the highest cytotoxic effect of Isochrysis galbana microalgae extract was shown at about 24.07±6.48% cytotoxicity against Raji cells.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Background and Aims: This study was aimed to investigate the anti-cancer effect of Isochrysis galbana microalgae extract.
📊
Key Findings & Data:Methods: In the study, the chemical composition of Isochrysis galbana microalgae extract was analyzed, and its cytotoxic effect against K562, HL60, U937, MOLT-4, and Raji cancer cells was investigated.
💡
Conclusion & Impact:To determine the effects of Isochrysis galbana extract, an MTT cytotoxicity assay was performed.Results: According to the results of the experiments, the highest cytotoxic effect of Isochrysis galbana microalgae extract was shown at about 24.07±6.48% cytotoxicity against Raji cells.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Microalgae · Cancer · Cytotoxicity

Abstract

Background and Aims: This study was aimed to investigate the anti-cancer effect of Isochrysis galbana microalgae extract. Methods: In the study, the chemical composition of Isochrysis galbana microalgae extract was analyzed, and its cytotoxic effect against K562, HL60, U937, MOLT-4, and Raji cancer cells was investigated. ECV304 endothelial cells were used as a healthy cell line for the understanding of its selective cytotoxicity. To determine the effects of Isochrysis galbana extract, an MTT cytotoxicity assay was performed.Results: According to the results of the experiments, the highest cytotoxic effect of Isochrysis galbana microalgae extract was shown at about 24.07±6.48% cytotoxicity against Raji cells. There were a large number of bioactive molecules in the ex- tract, and these molecules showed a specific response to Raji cells when considering the synergistic and antagonistic effects of these molecules on each other.Conclusion: According to the results of GC-MS analysis of Isochrysis galbana microalgae extract, the most intense molecules in the content were Dodecanoic acid, 3-hydroxy- (CAS) Beta-Hydroxy Dodecanoic Acid, and Propanoic acid, 2-methyl-, 1-(1,1-dimethylethyl)-2-methyl-1,3-isonide. The investigation of the effect of these molecules specifically against Raji cells is important to determine the possible anti-leukemic molecules and their combinations that show cytotoxicity against this cell line.

🔖

Cite This Article

ARSLAN, B. A., YILANCIOĞLU, K., GÜLTEKİN, S. K., ALBAYRAK, İ. G. (2022). Chemical constituent of Isochrysis galbana microalgae extract and its cytotoxic activities on leukemic cell lines. İstanbul Journal of Pharmacy, Cilt: 52 - Sayı: 1, 64–68. https://ajindex.com/articles/676222

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (4)

  1. Arslan, B.A., Isik, F.B., Gur, H., Ozen, F., & Catal, T. (2017). Apoptotic effect of Nigella sativa on human lymphoma U937 cells. Phar- macognosy Magazine, 13(3), 628-632. https://doi.org/10.4103/ PM.PM_93_17 Barata, J.T., Silva, A., Brandao, J.G., Nadler, L. M., Cardoso, A.A., & Boussiotis, V. A. (2004). Activation of PI3K is indispensable for interleukin 7-mediated viability, proliferation, glucose use, and growth of T cell acute lymphoblastic leukemia cells. The Journal of Experimental Medicine, 200(5), 659–669. https://doi.org/10.1084/ JEM.20040789 Batista, A.P., Gouveia, L., Bandarra, N.M., Franco, J.M., & Raymundo,A. (2013). Comparison of microalgal biomass profiles as novel functional ingredient for food products. Algal Research, 2(2), 164– 173. https://doi.org/10.1016/J.ALGAL.2013.01.004
  2. Bechelli, J., Coppage, M., Rosell, K., & Liesveld, J. (2011). Cy- totoxicity of algae extracts on normal and malignant cells. Leukemia Research and Treatment, 2011, 1–7. https://doi. org/10.4061/2011/373519 Becker, E. W. (2003). Microalgae for human and animal nutrition. Amos Richmond (Eds.), Handbook of Microalgal Culture: Applied Phycology and Biotechnology(pp. 461–503).New Jersey: Blackwell Publishing Ltd. https://doi.org/10.1002/9781118567166.CH25
  3. Ferdous, U.T., Norhana, Z., & Yusof, B. (2021). Algal terpenoids: A po- tential source of antioxidants for cancer therapy. Frontiers in Phar- macology, 12, 1-22.https://doi.org/10.5772/INTECHOPEN.94122
  4. Folmer, F., Jaspars, M., Solano, G., Cristofanon, S., Henry, E., Tabu- dravu, J. … Diederich, M. (2009). The inhibition of TNF-alpha- induced NF-kappaB activation by marine natural products. Bio- chemical Pharmacology, 78(6), 592–606. https://doi.org/10.1016/J. BCP.2009.05.009 Gamal-Eldeen, A.M., Ahmed, E.F., & Abo-Zeid, M.A. (2009). In vitro cancer chemopreventive properties of polysaccharide extract from the brown alga, Sargassum latifolium. Food and Chemical Toxicology: An International Journal Published for the British Indus- trial Biological Research Association, 47(6), 1378–1384. https://doi. org/10.1016/J.FCT.2009.03.016 Hafsa, M.B., Ismail, M.B., Garrab, M., Aly, R., Gagnon, J., & Naghmou- chi, K. (2017). Antimicrobial, antioxidant, cytotoxic and anticho- linesterase activities of water-soluble polysaccharides extracted from microalgae Isochrysis galbana and Nannochloropsis oculata. Journal of the Serbian Chemical Society, 82(5), 509–522. https://doi. org/10.2298/JSC161016036B Harada, H., & Kamei, Y. (1997). Se