Journals / Türk Tarım ve Doğa Bilimleri Dergisi / 2020 / Cilt: 7 - Sayı: 4

In vitro Storage of Peking Duck Semen in Different Diluents at + 5 °C

Pekin Ördeği Semeninin Farklı Sulandırıcılarda +5 °C' de in vitro Saklanması

Pages
1018–1025
Publication Date
2022-11-06
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In this study, it wasdetermined that the glucose diluent gives positive results compared to other diluents for the storage of pekingduck semen at +5 ° C for 72 hours and it can be used for short term storage as a semen diluent in ducks.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, it was aimed to investigate the storage possibilities of Peking duck (Anas platyrhynchosdomesticus) semen with different diluents at +5 ° C.
📊
Key Findings & Data:In the research, 50 semen samples were used.Semen from ducks were combined (mixed) after being evaluated individually.
💡
Conclusion & Impact:In this study, it wasdetermined that the glucose diluent gives positive results compared to other diluents for the storage of pekingduck semen at +5 ° C for 72 hours and it can be used for short term storage as a semen diluent in ducks.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, it was aimed to investigate the storage possibilities of Peking duck (Anas platyrhynchosdomesticus) semen with different diluents at +5 ° C. Semen samples were collected from five male ducks, twicea week for five weeks, using the abdominal massage method. In the research, 50 semen samples were used.Semen from ducks were combined (mixed) after being evaluated individually. Mix volume (ml), density (×109/mL), pH, motility (%) and vitality (%) values were determined as 1.78 ± 0.19, 1.65 ± 0.24, 6.95 ± 0.28, 70.80± 2.57, 76.60 ± 2.93 respectively. Glucose (G), Lactated Ringer's (L), Tris (T), Fructose (F), and Dextrose (D) wereused as diluents. The reconstituted mixed semen were equilibrated at 37 ° C for 30 minutes and 32-34 ° C for30 minutes successively and then stored at +5 °C. Average motility (%) values of sperms stored at +5 °C with G,L, T, F and D diluents after 72 hours were found as 41.05 ± 1.79, 9.95 ± 6.50, 2.20 ± 1.13, 28.00 ± 4.15,24.05±5.85 respectively. Vitality (%) values in the same diluents and under the same conditions were seen as44.82 ± 1.73, 19.60 ± 9.08, 10.60 ± 4.50, 35.90 ± 4.83 and 33.90 ± 2.68 respectively. In this study, it wasdetermined that the glucose diluent gives positive results compared to other diluents for the storage of pekingduck semen at +5 ° C for 72 hours and it can be used for short term storage as a semen diluent in ducks.

Özet

Bu çalışmada; Pekin ördeği (Anas platyrhynchos domesticus) semeninin farklı sulandırıcılarla +5°C'de muhafaza edilebilme olanaklarının araştırılması amaçlanmıştır. Semen örnekleri, beş adet erkek ördekten beş hafta süresince haftada iki kez olmak üzere, abdominal masaj yöntemi ile toplanmıştır. Araştırmada 50 semen örneği kullanılmıştır. Ördeklerden alınan semenler bireysel olarak değerlendirildikten sonra birleştirildi (miks yapıldı). Miks semenin volüm (ml), yoğunluk (×109 /mL), pH, motilite (%) ve vitalite (%) değerleri sırasıyla 1.78±0.19, 1.65±0.24, 6.95±0.28, 70.80±2.57, 76.60±2.93 olarak tespit edilmiştir. Sulandırıcı olarak; Glikoz (G), Laktatlı Ringer (L), Tris (T), Fruktoz (F), Dekstroz (D) sulandırıcıları kullanılmıştır. Sulandırılan miks semenler sırasıyla 37 °C'de 30 dakika ve 32-34 °C'de 30 dakika ekilibrasyona tabi tutulduktan sonra, +5 °C'de saklanmıştır. G, L, T, F ve D sulandırıcıları ile +5 °C'de saklanan spermaların 72 saat sonra ortalama motilite (%) değerleri sırasıyla 41.05±1.79, 9.95±6.50, 2.20±1.13, 28.00±4.15, 24.05±5.85 olarak bulundu. Aynı sulandırıcılardave aynı şartlarda vitalite (%) değerleri ise; sırasıyla 44.82±1.73, 19.60±9.08, 10.60±4.50, 35.90±4.83 ve 33.90±2.68 olarak tespit edilmiştir.Bu çalışmada pekin ördeği semeninin +5 °C'de 72 saat süreli saklanmasında glikoz sulandırıcısının diğer sulandırıcılara göre iyi sonuç verdiği ve ördeklerde semen sulandırıcısı olarak kısa süreli saklamalarda kullanılabilir olduğu tespit edilmiştir.

🔖

Cite This Article

KARADAVUT, U., TAŞKIN, A., ERGUN, F., ERGUN, D. (2022). In vitro Storage of Peking Duck Semen in Different Diluents at + 5 °C. Türk Tarım ve Doğa Bilimleri Dergisi, Cilt: 7 - Sayı: 4, 1018–1025. https://ajindex.com/articles/655358

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (12)

  1. Akpınar, G.Ç., Alaşahan, S.S. and Canoğulları, D. 2017. Halk elinde yetiştirilen Pekin ördeklerinde matematiksel formüller ile yumurta kalite özelliklerinin belirlenmesi openaccess.mku.edu.tr. Alkan, S., Baran A., Özdaş, B. and Evecen, M. 2002. Morphological defects in turkey semen. Turk. J. Vet. Anim. Sci. 26, 1087-1092.
  2. Ashizawa, K. and Okauchi, K. 1984. Stimulation of sperm motility and oxygen consuption f fowl spermatozoa by a low molecular weight fraction of seminal plasma. J. Reprod. Fertil. 71(2), 593-598.
  3. Bandyopadhyaya, S.K., Bhatacharya, Choudhury RR, Basu S. 2007. Text book of gynaecology, artificial insemination, obstetrics and assisted reproduction. 2nd ed. India: Kalyani Publishers/Lyall Bk Depot. Blesbois, E. 2003. Semen storage in turkeys: current status and future practice. In Fifth international symposium on turkey reproduction. Raleigh, USA. pp. 96–100.
  4. Blesbois, E. and Caffin, J.P. 1992. “Serum Like” Albumin of fowl seminal plasma and effects of albumin on fowl spermatozoa stored at 4°C. Br. Poult. Sci. 33(3), 663-670.
  5. Blesbois, E., Dubos, F., Grasseau, I., Richard, M.M., Roman, Y., Saint, J.M. and Seigneurin, F. 2006. Cryopreservation of avian spermatozoa and predictors of ability to freezing. Les Actes du BRG 6, 415–431.
  6. Clarke, R.N., Sexton, T.J. and Ottinger, M.A. 1982. Effects of holding temperature on storage time, on respiratory rate, motility and fertility of chicken and turkey semen. Poult. Sci. 61, 1912-1917.
  7. Cyriac, S., Joseph, L., Peethambaran, P.A., Narayanankutty, K. and Karthıayını, K. 2013. Semen quality characteristics of White Pekin, Kuttanad (Anas platyrhynchos domesticus) and Muscovy (Cairina moschata momelanotus) drakes. Indian J. Anim. Sci. 83(6), 595-599.
  8. Etches, R. J. (1996). Artificial insemination. In: Reprod poult. Cambridge. Wallingford: CAB International, 234-262.
  9. Fujihara, N. and Nishiyama, H. 1976. Studies on the accessory reproductive organs in the drake. 5. Effects of the fluid from the ejaculatory groove on the spermatozoa of the drake. Poult Sci. 55, 2415-2417
  10. Ghonim, A.I.A., Awad, A.L., El-Sawy, M.A., Fatouh, M.H. and Zenat, A.I. 2009. Effect of frequency of semen collection, dilution rate and insemination dose on semen characteristics and fertility of Domyati ducks. Egypt. Poult. Sci. J. 29, 1023-1045.
  11. Giesen, A.F. and Sexton, T.J. 1983. Beltsville poultry semen extender. 9. Effect of storage temperature on turkey semen held eighteen hours. Poult Sci. 62(7), 1305-1311.
  12. İnci, H., Karakaya, E., Şengül,