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Journals / Türk Biyokimya Dergisi / 2017 / Cilt: 42 - Sayı: 4

Alchillea millefolium’un antioksidan kapasitesi, antiasetilkolinesteraz aktivitesi ve fare beyin homojenatında lipid peroksidasyonu üzerine inhibitör etkisi

Antioxidant capacity, anti-acetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium

Feride Sena SEZEN
Arzu ÖZEL
Sercan YILDIRIM
Ahmet YAŞAR
Sevda TÜRKİŞ
Elif Nur BARUT
Seçkin ENGİN
Burak BARUT
Pages
493–502
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The aim of our study was to investigate antioxidant and anti-acetylcholinesterase activities of A. millefolium extracts to determine its potential therapeutic usage in Alzheimer’s disease (AD).Methods: Methanol extracts (ME) and ethanol extracts (EE) of A. millefolium were prepared to determine (a) in vitro antioxidant activities, (b) the effects on acetylcholinesterase kinetics (by using a colorimetric spectroscopic method) and (c) the effects on sodium nitroprusside (SNP)-induced lipid peroxidation in mice brain homogenate

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Achillea millefolium (A. millefolium) is a traditional herbal medicine that contains natural compounds with antioxidant activities and is used for a wide range of conditions among people.
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Key Findings & Data:Objective: Achillea millefolium (A. millefolium) is a traditional herbal medicine that contains natural compounds with antioxidant activities and is used for a wide range of conditions among people.
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Conclusion & Impact:The aim of our study was to investigate antioxidant and anti-acetylcholinesterase activities of A. millefolium extracts to determine its potential therapeutic usage in Alzheimer’s disease (AD).Methods: Methanol extracts (ME) and ethanol extracts (EE) of A. millefolium were prepared to determine (a) in vitro antioxidant activities, (b) the effects on acetylcholinesterase kinetics (by using a colorimetric spectroscopic method) and (c) the effects on sodium nitroprusside (SNP)-induced lipid peroxidation in mice brain homogenate
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Achillea millefolium (A. millefolium) is a traditional herbal medicine that contains natural compounds with antioxidant activities and is used for a wide range of conditions among people. The aim of our study was to investigate antioxidant and anti-acetylcholinesterase activities of A. millefolium extracts to determine its potential therapeutic usage in Alzheimer’s disease (AD).Methods: Methanol extracts (ME) and ethanol extracts (EE) of A. millefolium were prepared to determine (a) in vitro antioxidant activities, (b) the effects on acetylcholinesterase kinetics (by using a colorimetric spectroscopic method) and (c) the effects on sodium nitroprusside (SNP)-induced lipid peroxidation in mice brain homogenate

Özet

Amaç: Achillea millefolium (A. millefolium), antioksidan aktiviteye sahip doğal bileşikler içeren ve halk arasında çeşitli amaçlar için kullanılan geleneksel tıbbi bir bitkidir Çalışmamızın amacı, A. millefolium ekstrelerinin Alzheimer hastalığının (AD) tedavisinde kullanılma potansiyelini ortaya koymak için gerekli antioksidan ve anti-asetilkolinesteraz aktivitelerini araştırmaktır.Metod: A. millefolium'un metanol (ME) ve etanol ekstraktları (EE) hazırlanarak, (a) in vitro antioksidan aktiviteleri, (b) Asetilkolinesteraz kinetiği üzerindeki etkileri (kolorimetrik spektroskopik yöntem kullanılarak) ve (c) Fare beyin homojenatında sodyum nitroprusiyat (SNP) ile indüklenen lipid peroksidasyona etkisi incelenmiştir.Bulgular: ME, EE'den daha yüksek antioksidan aktiviteye sahiptir. Her iki ekstrakt da yarışmalı asetilkolinesteraz inhibisyonuna neden olmaktadır; ancak ME'nin bu inhibitor etkisi EE'den daha yüksektir. ME ve EE'nin inhibitor sabitleri (Ki) sırasıyla 28.43 ve 68.47 ?g/mL olarak bulunmuştur. Her iki ekstrakt da fare beyin homojenatındaki malondialdehit (MDA) düzeylerinde konsantrasyona bağlı olarak anlamlı bir azalmaya neden olmuş ve bu durum güçlü bir lipid peroksidasyonunun inhibisyonunu göstermektedir.Sonuç: Bu sonuçlar A. millefolium'un sahip olduğu yüksek antioksidan kapasite ve anti-asetilkolinesteraz aktivitesinin taşıdığı fenolik bileşiklerden kaynaklandığı ve bu sebeple AD gibi nörodejeneratif hastalıklarda destekleyici tedavi yaklaşımında doğal terapötik bir ajan olarak kullanılabilirliği gösterilmiştir

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Cite This Article

SEZEN, F. S., ÖZEL, A., YILDIRIM, S., YAŞAR, A., TÜRKİŞ, S., BARUT, E. N., ENGİN, S., BARUT, B. (2022). Alchillea millefolium’un antioksidan kapasitesi, antiasetilkolinesteraz aktivitesi ve fare beyin homojenatında lipid peroksidasyonu üzerine inhibitör etkisi. Türk Biyokimya Dergisi, Cilt: 42 - Sayı: 4, 493–502. https://ajindex.com/articles/654724

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