SavedAdd your journal

Journals / Turkish Journal of Biology / 2017 / Cilt: 41 - Sayı: 5

N-Acetylglucoseamine modified alginate sponges as scaffolds for skin tissue engineering

Murat DEMİRBİLEK
Selçuk AKTÜRK
Nelisa LAÇİN TÜRKOĞLU
Pages
796–807
Publication Date
2022-11-05
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to the results, increasing the NAG concentration led to an increase in the swelling and nitrogen ratios in the hydrogels.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In the treatment of dermal wounds, wound-dressing materials prepared from natural mucopolysaccharides are widely used because of their advantages such as nonirritation, nontoxicity, and ease in topical application.
📊
Key Findings & Data:Anti- and proinflammatory cytokine levels of human monocyte-macrophage cells (THP-1) stimulated with hydrogels were determined.
💡
Conclusion & Impact:According to the results, increasing the NAG concentration led to an increase in the swelling and nitrogen ratios in the hydrogels.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In the treatment of dermal wounds, wound-dressing materials prepared from natural mucopolysaccharides are widely used because of their advantages such as nonirritation, nontoxicity, and ease in topical application. In the present study, alginate hydrogels modified with N-acetyl glucose amine (NAG) were prepared as wound-dressing material. Physical, chemical, thermal, and mechanical properties of the hydrogels were studied. Cytotoxicity of the hydrogels on endothelial (HUVEC) and keratinocyte (HaCaT) cells were examined. Anti- and proinflammatory cytokine levels of human monocyte-macrophage cells (THP-1) stimulated with hydrogels were determined. According to the results, increasing the NAG concentration led to an increase in the swelling and nitrogen ratios in the hydrogels. Additionally, increasing the NAG concentration decreased elastic modulus and degradation time. Hydrogels were not cytotoxic on HaCaT and HUVEC cells. It stimulated IL-10 and TNF-alpha levels at a small rate.

🔖

Cite This Article

DEMİRBİLEK, M., AKTÜRK, S., TÜRKOĞLU, N. L. (2022). N-Acetylglucoseamine modified alginate sponges as scaffolds for skin tissue engineering. Turkish Journal of Biology, Cilt: 41 - Sayı: 5, 796–807. https://ajindex.com/articles/654406

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (14)

  1. Asada M, Sugie M, Inoue M, Nakagomı K, Hongo S, Murata K, Tomizuka N (1997). Inhibitory effect of alginic acids on hyaluronidase and on histamine release from mast cells. Biosci Biotech Bioch 61: 1030-1032.
  2. Ates A, Kinikli G, Turgay M, Duman M (2004). The efficacy of viscosupplementation therapy with sodium hyaluronate in patients with knee osteoarthritis. Turk J Geriatr 7: 21-24.
  3. Balakrishnan B, Mohanty M, Umashankar P, Jayakrishnan A (2005). Evaluation of an in situ forming hydrogel wound dressing based on oxidized alginate and gelatin. Biomaterials 26: 6335-6342.
  4. Boeckel DG, Shinkai RSA, Grossi ML, Teixeira ER (2014). In vitro evaluation of cytotoxicity of hyaluronic acid as an extracellular matrix on OFCOL II cells by the MTT assay. Oral Surg Oral Med Oral Pathol Oral Radiol 117: 423-428.
  5. Boehler RM, Graham JG, Shea LD (2011). Tissue engineering tools for modulation of the immune response. Biotechniques 51: 239-244.
  6. Catanzano O, D’esposito V, Acierno S, Ambrosio M, De Caro C, Avagliano C, Ungaro F (2015). Alginate–hyaluronan composite hydrogels accelerate wound healing process. Carbohydr Polym 131: 407-414.
  7. Chang C, Duan B, Zhang L (2009). Fabrication and characterization of novel macroporous cellulose–alginate hydrogels. Polymer 50: 5467-5473.
  8. Chaudhuri O, Gu L, Darnell M, Klumpers D, Bencherif SA, Weaver JC, Mooney DJ (2015). Substrate stress relaxation regulates cell spreading. Nat Commun 6: 7365-7372.
  9. Croce M, Boraldi F, Quaglino D, Tiozzo R, Pasquali-Ronchetti I (2003). Hyaluronan uptake by adult human skin fibroblasts in vitro. Eur J Histochem 47: 63-73.
  10. Croce M, Dyne K, Boraldi F, Quaglino D, Cetta G, Tiozzo R, Ronchetti IP (2001). Hyaluronan affects protein and collagen synthesis by in vitro human skin fibroblasts. Tissue Cell 33: 326-331.
  11. Das D, Pal S (2015). Modified biopolymer-dextrin based crosslinked hydrogels: application in controlled drug delivery. RSC Adv 5: 25014-25050.
  12. Demidova-Rice TN, Hamblin MR, Herman IM (2012). Acute and impaired wound healing: pathophysiology and current methods for drug delivery, part 1: normal and chronic wounds: biology, causes, and approaches to care. Adv Skin Wound Care 25: 304-314.
  13. Draget KI, Skjåk-Bræk G, Smidsrød O (1997). Alginate based new materials. Int J Biol Macromol 21: 47-55.
  14. Dvir-Ginzberg M, Gamlieli-Bonshtein I, Agbaria R, Cohen S (2003). Liver tissue engineering within alginate scaffolds: effects of cell-seeding density on hepatocyte viability, morphology, and function. Tissue Eng 9: 757-766.