Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines

Objective & Hypothesis:Laurocerasus officinalis Roem. (Prunus laurocerasus l.), also locally known as “karayemis or taflan”, is a popular black summer fruit belonging to the genus Prunus and the family Rosaceae.

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Key Findings & Data:Total phenolic content value was 33.7±0.13 mg gallic acid equivalent per g sample.

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Conclusion & Impact:Further studies are now needed to clarify the molecules involved and their mechanisms.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Domain Parameters

Abstract

Laurocerasus officinalis Roem. (Prunus laurocerasus l.), also locally known as “karayemis or taflan”, is a popular black summer fruit belonging to the genus Prunus and the family Rosaceae. L. officinalis exhibits numerous biological activities, such as anti-inflammatory, antinociceptive, antioxidant, neuroprotective, and antidiabetic effects. The aim of this study was to determine the total phenolic content and cytotoxic effect of dimethyl sulfoxide extract of L. officinalis (DEl). The total phenolic content and cytotoxic effect of DEl were investigated using the Folin-ciocalteu method and thiazolyl blue tetrazolium bromide (MTT) assay, respectively. Total phenolic content value was 33.7±0.13 mg gallic acid equivalent per g sample. DEl exhibited selective cytotoxic effects against four human cancer (lung, colon, liver, and cervix) cell lines. The most selective cytotoxic effect of the extract was observed in colon cancer cells (Ic50=265.2±8.1 µg/ml) compared to normal fibroblast cells. These data demonstrate that L. officinalis extract exhibits selective cytotoxicity towards some cancer cells. Further studies are now needed to clarify the molecules involved and their mechanisms.

Özet

Laurocerasus officinalis Roem. (Prunus laurocerasus l.), yerel olarak karayemiş veya taflan olarak da bilinir, Prunus cinsi ve Rosaceae familyasına mensup, koyu renkli bir yaz meyvesidir. L. officinalis, anti-inflamatuvar, antinosiseptif, antioksidan, nöroprotektif ve antidiyabetik gibi çeşitli biyolojik aktiviteler göstermektedir. Bu çalışmanın amacı, L. officinalis’in dimetil sülfoksitli ekstraktının antioksidan ve sitotoksik etkilerini belirlemektir. Ekstraktın toplam polifenol içeriği Folin ciocalteu metodu, sitotoksik etkinliği ise MTT yöntemiyle belirlendi. Ekstraktın toplam polifenol içeriği 33.7±0.13 mg gallik asit ekivalanı/g örnek olarak bulundu. Ekstrakt akciğer, kolon, karaciğer ve serviks kanser hücre serilerinde seçici bir sitotoksik etki gösterdi. normal fibroblast hücreleri ile karşılaştırıldığında en etkili seçici sitotoksik etkinin kolon kanseri hücrelerinde olduğu belirlendi (Ic50=265.2±8.1 µg/ml). Sonuçlar L. officinalis ekstraktının bazı kanser hücre serilerinde seçici bir sitotoksik etki gösterebildiğini ortaya koymaktadır. Ekstrakttaki etken molekülleri ve bunların etki mekanizmalarını belirleyebilmek için daha ileri çalışmalara ihtiyaç vardır.

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Cite This Article

TURAN, İ., ALİYAZICIOĞLU, Y., DEMİR, S., DEMİR, E. A., DEMİR, F. (2022). Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines. Marmara Pharmaceutical Journal, Cilt: 21 - Sayı: 1, 121–126. https://ajindex.com/articles/653144

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