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Journals / Marmara Pharmaceutical Journal / 2017 / Cilt: 21 - Sayı: 1

Cytotoxicity of triterpenes from the leaves of Garcinia prainiana King (Guttiferae)

Garcinia prainiana King (Guttiferae) Yapraklarından Elde Edilen Triterpenlerin Sitotoksik Özellikleri

Hasnah Mohd SİRAT
Muhammad TAHER
Shamsul ON
Wan Mohd Nuzul Hakimi Wan SALLEH
Farediah AHMAD
Pages
127–131
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

All compounds were tested for cytotoxicity against H1299 and A549 lung cancer cell lines using MTT assay. 3β-Acetoxyeupha-5,22diene 5 exhibited the most significant activity against human non-small cell lung carcinoma cell lines, H1299 and A549 with IC50 values 18.0 µg/mL and 36.3 µg/mL, respectively.

📌 3 Key Takeaways

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Objective & Hypothesis:Phytochemical investigation of the leaves of Garcinia prainiana resulted in the isolation of five triterpenes identified as squalene 1, friedelin 2, lupeol 3, 3β-hydroxyeupha-5,22-diene 4, and 3β-acetoxyeupha-5,22-diene 5.
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Key Findings & Data:The structures of these compounds were established by analysis of their spectroscopic data, as compared to that of reported compounds.
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Conclusion & Impact:All compounds were tested for cytotoxicity against H1299 and A549 lung cancer cell lines using MTT assay. 3β-Acetoxyeupha-5,22diene 5 exhibited the most significant activity against human non-small cell lung carcinoma cell lines, H1299 and A549 with IC50 values 18.0 µg/mL and 36.3 µg/mL, respectively.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Phytochemical investigation of the leaves of Garcinia prainiana resulted in the isolation of five triterpenes identified as squalene 1, friedelin 2, lupeol 3, 3β-hydroxyeupha-5,22-diene 4, and 3β-acetoxyeupha-5,22-diene 5. The structures of these compounds were established by analysis of their spectroscopic data, as compared to that of reported compounds. All compounds were tested for cytotoxicity against H1299 and A549 lung cancer cell lines using MTT assay. 3β-Acetoxyeupha-5,22diene 5 exhibited the most significant activity against human non-small cell lung carcinoma cell lines, H1299 and A549 with IC50 values 18.0 µg/mL and 36.3 µg/mL, respectively.

Özet

Garcinia prainiana yapraklarını konu alan fitokimyasal araştırmalar sonucunda triterpen yapısında beş bileşik izole edilmiştir; skualen 1, friedelin 2, lupeol 3, 3β-hidroksiöfa5,22-dien 4, and 3β-asetoksiöfa-5,22-dien 5. İzole edilen bileşiklerin yapıları spektroskopik yöntemler kullanılarak aydınlatılmış ve elde edilen sonuçlar yukarıda belirtilen bileşiklere ait spektroskopik verilerle karşılaştırılmıştır. Tüm bileşiklerin, H1299 ve A549 akciğer kanseri hücre hatları üzerindeki sitotoksik etkileri MTT yöntemiyle çalışılmıştır. 3β-Asetoksiöfa-5,22-dien 5, H1299 ve A549 hücre hatlarına karşı sırasıyla IC50 = 18.0 µg/ml ve IC50= 36.3 µg/ml derişimde etki göstererek serisindeki en etkili bileşik olarak öne çıkmıştır.

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Cite This Article

SİRAT, H. M., TAHER, M., ON, S., SALLEH, W. M. N. H. W., AHMAD, F. (2022). Cytotoxicity of triterpenes from the leaves of Garcinia prainiana King (Guttiferae). Marmara Pharmaceutical Journal, Cilt: 21 - Sayı: 1, 127–131. https://ajindex.com/articles/578548

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