Journals / Lalahan Hayvancılık Araştırma Enstitüsü Dergisi / 2005 / Cilt: 45 - Sayı: 1

The effect of estrous cow serum and fetal calf serum on in vitro embryo production

Östrustaki inek serumu ve fötal buzağı serumunun in vitro embriyo elde edilmesine etkisi

Pages
1–6
Publication Date
2022-11-05
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result, the effect of fetal calf sera (FCS) was superior than estrous cow sera (ECS) for in vitro development into morulae-blastocyst stage of bovine oocytes.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to compare influence of estrous cow sera (ECS) and fetal calf sera (FCS) on in vitro embryo production.
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Key Findings & Data:Estrous Cow Serum was added in the maturation media 5%, 10% and 20%.
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Conclusion & Impact:As a result, the effect of fetal calf sera (FCS) was superior than estrous cow sera (ECS) for in vitro development into morulae-blastocyst stage of bovine oocytes.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to compare influence of estrous cow sera (ECS) and fetal calf sera (FCS) on in vitro embryo production. Oocytes were selected and used as morphologically A and B quality grade in this study. Estrous Cow Serum was added in the maturation media 5%, 10% and 20%. The same proportions of FCS were added in the control groups. Embryos were cultured up to morula-blastocyst stage. Data were tested by two way ANOVA. The proportion of coming into morulae-blastocyst stage were 0.12±0.023, 0.15±0.023, 0.15±0.020 for ECS and 0.17±0.036, 0.24±0.045, 0.19±0.025 for FCS for 5%, 10% and 20% levels, respectively. As a result, the effect of fetal calf sera (FCS) was superior than estrous cow sera (ECS) for in vitro development into morulae-blastocyst stage of bovine oocytes.

Özet

Bu çalışmada, in vitro maturasyon medrumuna-eklenen Östrustaki İnek Serumu (ECS) ve Fötal Buzağı Serumunun (FCS) in vitro embriyo elde edilmesine etkisinin karşılaştırılması amaçlanmıştır. Çalışmada, morfolojik sınıflandırmaya göre A ve B kalite inek oositleri kullanılmıştır. Maturasyon mediumlarına, %5, %10 ve %20 oranlarında ECS; kontrol grubunda ise aynı oranlarda FCS ilave edilmiş ve embriyolar morula-blastosist evresine kadar kültüre edilmiştir. Veriler çift yönlü varyans analizi kullanılarak test edilmiştir. Çalışmada, ECS serum ekleme oranlarına göre sırasıyla; 0.12±0.023, 0.15±0.023 ve 0.15±0.020; FCS'de ise 0.17±0.036, 0.24±0.045 ve 0.19±0.025 morula-blastosiste ulaşma oranı elde edilmiştir. Sonuç olarak, Fötal Buzağı Serumunun (FCS), in vitro morula-blastosist elde etmede Östrustaki İnek Serumuna (ECS) göre daha üstün olduğu görülmüştür.

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Cite This Article

SULU, N., AKYOL, N. (2022). The effect of estrous cow serum and fetal calf serum on in vitro embryo production. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, Cilt: 45 - Sayı: 1, 1–6. https://ajindex.com/articles/653073

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References (2)

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