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Journals / İzmir Tepecik Eğitim Hastanesi Dergisi / 2019 / Cilt: 29 - Sayı: 1

The Effect of Alpha Lipoic Acid on Oxidative Stress in the Rat Experimental Colitis Model

Ratlarda Deneysel Kolit Modelinde Alfa Lipoik Asit Kullanımının Oksidatif Stres Üzerine Etkisi

Aslıhan BÜYÜKÖZTÜRK KARUL
Mesut YAZICI
Mustafa Onur ÖZTAN
Nil ÇULHACI
Sezen KARACA ÖZKISACIK
Mustafa Harun GÜRSOY
Pages
47–55
Publication Date
2022-11-05
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

Nosignificant pathological mucosal changes were detected in ALA Gavage Group, and significantly lower macroscopicscores and blood MDA levels were found when compared to Sham Group (p

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Ulcerative colitis is an immonologically mediated chronic intestinal disorder, which is characterized withinflammation, oxidative stress, apoptosis.
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Key Findings & Data:After induction of colitis with trinitrobenzene sulfonic acid,and corn oil via gavage (Sham Group),alpha lipoic acid 200 mg/kg/day via gavage (ALA Gavage Group), intracolonic alpha lipoic acid 200 mg/kg/day(ALA Enema Group), and intracolonic corn oil (Corn Oil Enema Group) were administrated.
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Conclusion & Impact:Nosignificant pathological mucosal changes were detected in ALA Gavage Group, and significantly lower macroscopicscores and blood MDA levels were found when compared to Sham Group (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Ulcerative colitis is an immonologically mediated chronic intestinal disorder, which is characterized withinflammation, oxidative stress, apoptosis. Our study was aimed to investigate the effects of alpha lipoic acid administered orally or as colonic enema in trinitrobenzene sulfonic acid-induced ulcerative colitis.Methods: Forty-two Wistar Albino rats were divided into five groups. Intracolonic corn oil was administered in theControl Group. After induction of colitis with trinitrobenzene sulfonic acid,and corn oil via gavage (Sham Group),alpha lipoic acid 200 mg/kg/day via gavage (ALA Gavage Group), intracolonic alpha lipoic acid 200 mg/kg/day(ALA Enema Group), and intracolonic corn oil (Corn Oil Enema Group) were administrated. On the eighth day of thestudy, colonic specimens were evaluated and scored for the presence of macroscopic and microscopic lesions.Results: In the microscopic evaluation, total scores of Sham and Corn Oil Enema Groups were higher than those ofthe Control Group (p0.05). Nosignificant pathological mucosal changes were detected in ALA Gavage Group, and significantly lower macroscopicscores and blood MDA levels were found when compared to Sham Group (p

Özet

Amaç: Ülseratif kolit; enflamasyon, oksidatif stres, apoptoz ile karakterize kronik bir gastrointestinal sistem bozukluğudur. Çalışmamızda amacımız, alfa lipoik asiti gavaj ve lavman yolu ile vererek trinitrobenzen sülfonik asit ile deneysel kolit modelindeki antioksidan etkisini araştırmak idi. Yöntem: Çalışmada, toplam 42 adet Wistar albino sıçan 5 gruba ayrıldı. Birinci gruptaki (kontrol grubu) ratlara 7 gün boyunca günde 1 kez 1 cc intrakolonik mısırözü yağı verildi. Diğer 4 gruptaki ratlarda intrakolonik 25 mg trinitrobenzen sülfonik asit + etanol uygulaması ile kolit oluşturuldu. Kolit oluşturulmasından 1 gün sonra başlanarak 7 gün boyunca ve günde 1 kez olmak üzere; ikinci gruba (Sham grubu) oral yoldan mısırözü, üçüncü gruba (ALA Gavaj grubu) oral yoldan 200 mg/kg/gün alfa lipoik asit, dördüncü gruba (ALA Lavman grubu) intrakolonik 200 mg/kg/ gün alfa lipoik asit ve beşinci gruba (MÖ Lavman grubu) ise intrakolonik 1 cc mısırözü yağı verildi. Çalışma sonunda örnekler histopatolojik ve biyokimyasal parametreler açısından değerlendirildiler. Bulgular: Toplam mikroskopik değerlendirme skorlarında Sham ve MÖ Lavman grubundaki değerler Kontrol grubuna oranla yüksek saptandı (p0,05). ALA Gavaj grubunda hiçbir mukozal patolojiye rastlanmazken, Sham grubu ile karşılaştırıldığında makroskopi ve MDA değerlerinde anlamlı düşüklük saptandı (p

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Cite This Article

KARUL, A. B., YAZICI, M., ÖZTAN, M. O., ÇULHACI, N., ÖZKISACIK, S. K., GÜRSOY, M. H. (2022). The Effect of Alpha Lipoic Acid on Oxidative Stress in the Rat Experimental Colitis Model. İzmir Tepecik Eğitim Hastanesi Dergisi, Cilt: 29 - Sayı: 1, 47–55. https://ajindex.com/articles/652210

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References (13)

  1. Podolsky DK. Inflammatory bowel disease. N Engl J Med. 2002;347(6):417-29.
  2. Shih DQ, Targan SR. Immunopathogenesis of inflammatory bowel disease. World J Gastroenterol. 2008;(3)14:390-400. Sartor RB. Mechanisms of disease: pathogenesis of Crohn’s disease and ulcerative colitis. Nat Clin Pract Gastroenterol Hepatol. 2006;3(7):390-407.
  3. Khor B, Gardet A, Xavier RJ. Genetics and pathogenesis of inflammatory bowel disease. Nature. 2011;474(7351):307- 17. Carvalho R, Hyams JS. Diagnosis and management of inflammatory bowel disease in children. Semin Pediatr Surg. 2007;16(3):164-71.
  4. Escher JC, Ten KF, Lichtenbelt K, Schornagel I, Buller H, Derkx B, et al. Value of rectosigmoidoscopy with biopsies for diagnosis of inflammatory bowel disease in children. Inflamm Bowel Dis. 2002;8(1):16-22.
  5. Irving PM, Gibson PR. Infections and IBD. Nat Clin Pract Gastroenterol Hepatol. 2008;5(1):18-27.
  6. Irving PM, Shanahan F, Rampton DS. Drug interactions in inflammatory bowel disease. Am J Gastroenterol. 2008;103(1):207-19.
  7. Karlen P, Lofberg R, Brostrom O, Leijonmarck CE, Hellers G, Persson PG. Increased risk of cancer in ulcerative colitis: a population-based cohort study. Am J Gastroenterol. 1999;94(4):1047-52.
  8. Biewenga GP, Haenen GR, Bast A. The pharmacology of the antioxidant lipoic acid. Gen Pharmacol. 1997;29(3):315-31.
  9. Bilska A, Wlodek L. Lipoic acid - the drug of the future? Pharmacol Rep. 2005;57:570-7. Available from: http://www. if-pan.krakow.pl/pjp/pdf/2005/5_570.pdf. Karakoyun B, Yuksel M, Ercan F, Erzik C, Yegen BC. Alphalipoic acid improves acetic acid-induced gastric ulcer healing in rats. Inflammation. 2009;32(1):37-46. [CrossRef] Sharma M, Gupta YK. Effect of alpha lipoic acid on intracerebroventricular streptozotocin model of cognitive impairment in rats. Eur Neuropsychopharmacol. 2003;13(4):241-7.
  10. Wengrower D, Zanninelli G, Pappo O, Latella G, Sestieri M, Villanova A, et al. Prevention of fibrosis in experimental colitis by captopril: the role of tgf-beta1. Inflamm Bowel Dis. 2004;10(5):536-45.
  11. Ek RO, Serter M, Ergin K, Yildiz Y, Cecen S, Kavak T, et al. The effects of caffeic acid phenethyl ester (CAPE) on TNBSinduced colitis in ovariectomized rats. Dig Dis Sci. 2008;53(6):1609-17.
  12. Peran L, Camuesco D, Comalada M, Nieto A, Concha A, DiazRopero MP, et al. Preventative effects of a probiotic, Lactobacillus salivarius ssp. salivarius, in the TNBS model of rat colitis. World J Gastroenterol. 2005;11(33):5185-92.
  13. Bell CJ, Gall DG, Wallace JL. Disruption of colonic ele