SavedAdd your journal

Journals / Cumhuriyet Dental Journal / 2019 / Cilt: 22 - Sayı: 1

EVALUATION OF CAFFEIC ACID PHENETHYL ESTER ADMINISTRATION IN CHRONICALLY STRESSED RATS WITH EXPERIMENTAL PERIODONTITIS

Deneysel Periodontitis Oluşturulan Kronik Stresli Sıçanlarda Kafeik Asit Fenetil Ester (KAFE) Uygulamasının Değerlendirilmesi

Ufuk Taşdemir
Alper Kızıldağ
Mukaddes Mergen Dalyanoğlu
Taner Arabacı
Mevlüt Albayrak
Canan Aksu Kızıldağ
Pages
114–120
Publication Date
2022-11-06
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Oxidative stress and interleukin (IL)-1β were investigated.The receptor activator of the nuclear factor kappa B ligand (RANKL) and alveolarbone loss were determined by immunohistochemical analysis.Results: The oxidative stress, alveolar bone loss, IL-1β and RANKL levels werefound significantly higher in the EP-CS group compared with control and EPgroups (p

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objectives: The aim of the present study was to investigate the therapeutic effectsof systemic caffeic acid phenethyl ester treatment on oxidative stress and alveolarbone destruction in lipopolysaccharide (LPS)-induced periodontitis (EP) group inchronically stressed rats.Materials and Methods: Fourty male Sprague Dawley rats (EP-CS-CAPE) groupwere divided into four groups: 1) control group, 2) experimental periodontitis (EP),3) EP and chronic stress (CS) group (EP-CS), and 4) EP-CS treated with CAPE(EP-CS-CAPE).
📊
Key Findings & Data:To induce periodontitis, LPS was administered into the buccalgingiva of the test groups, and pure saline was administered for the interleukin (IL)-1β levels were control group.
💡
Conclusion & Impact:Oxidative stress and interleukin (IL)-1β were investigated.The receptor activator of the nuclear factor kappa B ligand (RANKL) and alveolarbone loss were determined by immunohistochemical analysis.Results: The oxidative stress, alveolar bone loss, IL-1β and RANKL levels werefound significantly higher in the EP-CS group compared with control and EPgroups (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: The aim of the present study was to investigate the therapeutic effectsof systemic caffeic acid phenethyl ester treatment on oxidative stress and alveolarbone destruction in lipopolysaccharide (LPS)-induced periodontitis (EP) group inchronically stressed rats.Materials and Methods: Fourty male Sprague Dawley rats (EP-CS-CAPE) groupwere divided into four groups: 1) control group, 2) experimental periodontitis (EP),3) EP and chronic stress (CS) group (EP-CS), and 4) EP-CS treated with CAPE(EP-CS-CAPE). To induce periodontitis, LPS was administered into the buccalgingiva of the test groups, and pure saline was administered for the interleukin (IL)-1β levels were control group. Two test groups were exposed to restraint stress andone group of these groups was treated with only a single dose of CAPE (10mmol/kg). Likewise, saline was administered in the control, EP, and EP-CS groups.After 14 days, serum samples were collected from the heart, and all rats weresacrificed for analyses. Oxidative stress and interleukin (IL)-1β were investigated.The receptor activator of the nuclear factor kappa B ligand (RANKL) and alveolarbone loss were determined by immunohistochemical analysis.Results: The oxidative stress, alveolar bone loss, IL-1β and RANKL levels werefound significantly higher in the EP-CS group compared with control and EPgroups (p

Özet

Amaç: Bu çalışmanın amacı sistemik kafeik asit fenetil ester (KAFE) uygulamasının lipopolisakkarit (LPS) ile periodontitis oluşturulan kronik stresli sıçanlarda oksidatif stres ve alveolar kemik kaybı üzerine terapötik etkisinin değerlendirilmesidir. Gereç ve Yöntemler: Çalışmamızda 40 adet Sprague Dawley sıçan kullanıldı ve bu sıçanlar dört gruba ayrıldı: 1) kontrol grubu, 2) deneysel periodontitis (DP) grubu, 3) DP ve kronik stres (KS) grubu (DP-KS) ve 4) KAFE ile tedavi edilen DPKS grup (DP-KS-KAFE). Periodontitis oluşturmak amacıyla LPS test gruplarında bukkal dişetine uygulandı ve aynı yöntemle kontrol grubuna salin verildi. Bununla birlikte test gruplarından ikisine kısıtlama stresi uygulandı ve bu gruplardan biri de günlük tek doz KAFE ile tedavi edildi (10 mmol/kg). Benzer şekilde kontrol ve interlökin (IL)-1β seviyeleri incelendi. DP ve DP-KS gruplarına da salin uygulandı. 14 gün sonra, hayvanların kalbinden serum örnekleri toplandı ve bütün hayvanlar analiz için sakrifiye edildi. Oksidatif stres, interlökin (IL)-1β incelendi. Reseptör aktivatör nükleer faktör kappa B ligand (RANKL) ve alveolar kemik kaybı immünohistokimyasal olarak değerlendirildi. Bulgular: Oksidatif stres, alveoar kemik kaybı, IL-1β ve RANKL seviyeleri DP-KS grubunda kontrol ve DP gruplarına göre anlamlı bir şekilde yüksek bulundu (p

🔖

Cite This Article

Taşdemir, U., Kızıldağ, A., Dalyanoğlu, M. M., Arabacı, T., Albayrak, M., Kızıldağ, C. A. (2022). EVALUATION OF CAFFEIC ACID PHENETHYL ESTER ADMINISTRATION IN CHRONICALLY STRESSED RATS WITH EXPERIMENTAL PERIODONTITIS. Cumhuriyet Dental Journal, Cilt: 22 - Sayı: 1, 114–120. https://ajindex.com/articles/649393

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (14)

  1. Slots J. Periodontology: past, present, perspectives. Periodontol 2000 2013;62:7-19
  2. Okoro CA, Strine TW, Eke PI, Dhingra SS, Balluz LS. The association between depression and anxiety and use of oral health services and tooth loss. Community Dent Oral Epidemiol 2012;40:134-144
  3. Rai B, Kaur J, Anand SC, Jacobs R. Salivary stress markers, stress, and periodontitis: a pilot study. J Periodontol 2011;82:287-292
  4. Gaspersic R, Stiblar-Martincic D, Skaleric U. Influence of restraint stress on ligature-induced periodontitis in rats. Eur J Oral Sci 2002;110:125-129
  5. Padgett DA, Marucha PT, Sheridan JF. Restraint stress slows cutaneous wound healing in mice. Brain Behav Immun 1998;12:64-73
  6. Allan SM, Tyrrell PJ, Rothwell NJ. Interleukin-1 and neuronal injury. Nat Rev Immunol 2005;5:629-640
  7. Simi A, Tsakiri N, Wang P, Rothwell NJ. Interleukin-1 and inflammatory neurodegeneration. Biochem Soc Trans 2007;35:1122-1126. 8. de Kloet ER, Joels M, Holsboer F. Stress and the brain: from adaptation to disease. Nat Rev Neurosci 2005;6:463-475
  8. You Z, Luo C, Zhang W, Chen Y, He J, Zhao Q, Zuo R, Wu Y. Pro- and anti-inflammatory cytokines expression in rat's brain and spleen exposed to chronic mild stress: involvement in depression. Behav Brain Res 2011;225:135-141
  9. Musazzi L, Milanese M, Farisello P, Zappettini S, Tardito D, Barbiero VS, Bonifacino T, Mallei A, Baldelli P, Racagni G, Raiteri M, Benfenati F, Bonanno G, Popoli M. Acute stress increases depolarizationevoked glutamate release in the rat prefrontal/frontal cortex: the dampening action of antidepressants. PLoS One 2010;5:e8566
  10. Hardingham GE, Fukunaga Y, Bading H. Extrasynaptic NMDARs oppose synaptic NMDARs by triggering CREB shut-off and cell death pathways. Nat Neurosci 2002;5:405-414
  11. Samarghandian S, Azimi-Nezhad M, Borji A, Farkhondeh T. Crocus sativus L.(saffron) extract reduces the extent of oxidative stress and proinflammatory state in aged rat kidney. Prog Nutr 2016;18:299-310
  12. Genco RJ, Ho AW, Kopman J, Grossi SG, Dunford RG, Tedesco LA. Models to evaluate the role of stress in periodontal disease. Ann Periodontol 1998;3:288- 302
  13. Akyol S, Ginis Z, Armutcu F, Ozturk G, Yigitoglu MR, Akyol O. The potential usage of caffeic acid phenethyl ester (CAPE) against chemotherapy-induced and radiotherapy-induced toxicity. Cell Biochem Funct 2012;30:438-443
  14. Gremy O, Benderitter M, Linard C. Caffeic acid phenethyl ester modifies the Th1/Th2 balance in ileal mucosa after