SavedAdd your journal

Journals / Viral Hepatitis Journal / 2019 / Cilt: 25 - Sayı: 3

Evaluation of Signal/Cut-off Ratio by Anti-hepatitis Delta Virus Enzyme Immunoassay Method in the Diagnosis of Hepatitis Delta Virus Infection

Hepatit Delta Virüs Enfeksiyonunun Tanısında Anti-Hepatit Delta Virüs Enzim İmmünoassay Yöntemi ile Sinyal/Cut-off Oranının Değerlendirilmesi

Nuran KARABULUT
Murat YAMAN
Ayfer BAKIR
Mustafa ÖNEL
Sema ALAÇAM
Ali AĞAÇFİDAN
Pages
97–100
Publication Date
2022-11-05
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The mean S/CO value of anti-HDV in HDV-RNA positive group (8.99±3.53) was significantly higher than HDV-RNA negative group (5.99±3.73) (p

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objectives: The hepatitis delta virus (HDV) is an enveloped, negative-sense, single-stranded RNA virus in the genus Deltavirus.
📊
Key Findings & Data:Materials and Methods: Between August 2014 and December 2018, 156 patients, who were detected hepatitis B surface antigen (HBsAg) and anti-HDV positivity and were analyzed HDV-RNA, were included in the study.
💡
Conclusion & Impact:The mean S/CO value of anti-HDV in HDV-RNA positive group (8.99±3.53) was significantly higher than HDV-RNA negative group (5.99±3.73) (p
🔬Design:Research Article
📍Scope:2014 Verileri
⚙️Variables:Domain Parameters

Abstract

Objectives: The hepatitis delta virus (HDV) is an enveloped, negative-sense, single-stranded RNA virus in the genus Deltavirus. In this study, it was aimed to evaluate the ratio of anti-HDV signal/ cut-off (S/CO) in the diagnosis of HDV infection in patients who were positive for anti-HDV. Materials and Methods: Between August 2014 and December 2018, 156 patients, who were detected hepatitis B surface antigen (HBsAg) and anti-HDV positivity and were analyzed HDV-RNA, were included in the study. Anti-HDV antibody and HBsAg tests were analyzed by micro-ELISA method in serum samples and HDV-RNA was studied by real-time polymerase chain reaction method in plasma samples. Results: HDV-RNA was detected in 42.9% (67/156) of the anti-HDV positive patients. The mean S/CO value of anti-HDV in HDV-RNA positive group (8.99±3.53) was significantly higher than HDV-RNA negative group (5.99±3.73) (p

Özet

Amaç: Hepatit delta virüsü (HDV), Deltavirus genusunda, zarflı, negatif polariteli, tek iplikli bir RNA virüsüdür. Bu çalışmada, anti-HDV pozitif olan hastalarda HDV enfeksiyonu tanısında anti-HDV sinyal/ cut-off (S/CO) oranının değerlendirilmesi amaçlandı. Gereç ve Yöntemler: Ağustos 2014-Aralık 2018 tarihleri arasında hepatit B yüzey antijeni (HBsAg) ve anti-HDV pozitif saptanan ve HDV-RNA testi çalışılmış toplam 156 hasta çalışmaya dahil edildi. Anti-HDV antikoru ve HBsAg testleri serum örneklerinde mikroELISA yöntemi ile, HDV-RNA testi ise plazma örneklerinde real-time polimeraz zincir reaksiyonu yöntemi ile çalışıldı. Bulgular: Anti-HDV pozitif hastaların %42,9’unda (67/156) HDVRNA saptandı. HDV-RNA pozitif grubun anti-HDV S/CO ortalaması (8,99±3,53), HDV-RNA negatif gruba (5,99±3,73) göre anlamlı olarak yüksek saptandı (p

🔖

Cite This Article

KARABULUT, N., YAMAN, M., BAKIR, A., ÖNEL, M., ALAÇAM, S., AĞAÇFİDAN, A. (2022). Evaluation of Signal/Cut-off Ratio by Anti-hepatitis Delta Virus Enzyme Immunoassay Method in the Diagnosis of Hepatitis Delta Virus Infection. Viral Hepatitis Journal, Cilt: 25 - Sayı: 3, 97–100. https://ajindex.com/articles/646377

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (13)

  1. Rizzetto M. The adventure of delta. Liver Int. 2016;36:135-140
  2. ICTV. 9th Report (2011). Virus Taxonomy: 2017 Release. Deltavirus [08.06.2019]. Available from:https://talk.ictvonline.org/ ictv-reports/ictv_9th_report/negative-senserna-viruses-2011/w/ negrna_viruses/211/deltavirus 3. Negro F. Hepatitis D virus coinfection and superinfection. Cold Spring Harb Perspect Med. 2014;4:a021550
  3. Hughes SA, Wedemeyer H, Harrison PM. Hepatitis delta virus. Lancet. 2011;378:73-85
  4. Scarponi CFO, Silva RDND, Souza Filho JA, Guerra MRL, Pedrosa MAF, Mol MPG. Hepatitis delta prevalence in South America: A systematic review and meta-analysis. Rev Soc Bras Med Trop. 2019;52:e20180289
  5. Butler EK, Rodgers MA, Coller KE, Barnaby D, Krilich E, Olivo A, Cassidy M, Mbanya D, Kaptue L, Ndembi N, Cloherty G. High prevalence of hepatitis delta virus in Cameroon. Sci Rep. 2018;8:11617
  6. Kemal Celen M, Tekin Koruk S, Aygen B, Dal T, Karabay O, Tosun S, Koksal I, Turgut H, Onlen Y, Balik I, Yildirim N, Sinan Dal M, Ayaz C, Tabak F. The characteristics of patients with chronic hepatitis B in Turkey. Med Glas (Zenica). 2014;11:94-98
  7. Özkan H. Epidemiology of chronic hepatitis B in Turkey. Euroasian J Hepatogastroenterol. 2018;8:73-74
  8. Botelho-Souza LF, Vasconcelos MPA, Dos Santos AO, Salcedo JMV, Vieira DS. Hepatitis delta: Virological and clinical aspects. Virol J. 2017;14:177
  9. Yurdaydın C, Tabak F, Kaymakoğlu S, Akarsu M, Akıncı EG, Akkız H, Alkım C, Çekin AH, Çuvalcı NÖ, Demir K, Değertekin B, Dökmetaş İ, Ersöz G, Hizel K, Kandemir FÖ, Önlen Y, Sonsuz A, Şenateş E, Tosun S, Tözün N, Idilman R, Guidelines Study Group VH. Diagnosis, management and treatment of hepatitis delta virus infection: Turkey 2017 Clinical Practice Guidelines. Turk J Gastroenterol. 2017;28:84-89
  10. Kamili S, Drobeniuc J, Mixson-Hayden T, Kodani M. Delta hepatitis: Toward improved diagnostics. Hepatology. 2017;66:1716-1718
  11. Brichler S, Le Gal F, Butt A, Chevret S, Gordien E. Commercial real-time reverse transcriptase PCR assays can underestimate or fail to quantify hepatitis delta virus viremia. Clin Gastroenterol Hepatol. 2013;11:734-740
  12. Brichler S, Le Gal F, Neri-Pinto F, Mansour W, Roulot D, Laperche S, Gordien E. Serological and molecular diagnosis of hepatitis delta virus infection: Results of a French national quality control study. J Clin Microbiol. 2014;52:1694-1697
  13. Mederacke I, Yurdaydin C, Dalekos GN, Bremer B, Erhardt A, Cakaloglu Y, Yalcin K, Gurel S, Zeuzem S