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Journals / Viral Hepatitis Journal / 2019 / Cilt: 25 - Sayı: 3

microRNA in Patients with Hepatitis B and Hepatitis C Virus Associated Hepatocellular Carcinoma and Cirrhosis

Hepatit B ve Hepatit C İlişkili Hepatosellüler Karsinom ve Siroz Olgularında microRNA

Mehmet KÖROĞLU
Bilal TOKA
Ferhat Gürkan ASLAN
Mustafa ALTINDİŞ
Gökhan KÜÇÜKKARA
Pages
113–116
Publication Date
2022-11-05
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Results: Hsa-miRNA-21-3p and hsa-miRNA-122-3p levels increased 3-4 fold in patients without HCV related LC (HCV-LC) and hsamiRNA-29a-3p expression in HCV infected patients has significantly decreased (p

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: The aim of this study was to determine the potentials of hsa-microRNAs (miRNA)-21-3p, hsa-miRNA-29a-3p, hsa-miRNA122-3p, hsa-miRNA-192-5p in Hepatitis B (HBV) and HCV related hepatocellular carcinoma (HCC) and liver cirrhosis (LC) cases, being biomarkers by examining their levels.
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Key Findings & Data:Materials and Methods: Sixty patients and 26 healthy volunteers were included in the study.
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Conclusion & Impact:Results: Hsa-miRNA-21-3p and hsa-miRNA-122-3p levels increased 3-4 fold in patients without HCV related LC (HCV-LC) and hsamiRNA-29a-3p expression in HCV infected patients has significantly decreased (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: The aim of this study was to determine the potentials of hsa-microRNAs (miRNA)-21-3p, hsa-miRNA-29a-3p, hsa-miRNA122-3p, hsa-miRNA-192-5p in Hepatitis B (HBV) and HCV related hepatocellular carcinoma (HCC) and liver cirrhosis (LC) cases, being biomarkers by examining their levels. Materials and Methods: Sixty patients and 26 healthy volunteers were included in the study. Total RNA isolation in the serum samples was performed with the Direct-zolTM RNA MiniPrep (Zymo Research Corp., USA) commercial kit followed by cDNA synthesis and realtime polymerase chain reaction amplification with EPIK miRNA Select Hi/Lo-ROX (Bioline Reagents Ltd., USA). For amplification and analysis, Rotor-Gene Q (QIAGEN, Germany) instrument was used and statistical analyzes were performed with SPSS 21 (IBM, USA) program. Results: Hsa-miRNA-21-3p and hsa-miRNA-122-3p levels increased 3-4 fold in patients without HCV related LC (HCV-LC) and hsamiRNA-29a-3p expression in HCV infected patients has significantly decreased (p

Özet

Amaç: Bu çalışmada hsa-mikroRNA (miRNA)-21-3p, hsa-miRNA29a-3p, hsa-miRNA-122-3p, hsa-miRNA-192-5p’nin Hepatit B (HBV) ve HCV ilişkili hepatosellüler karsinoma (HCC) ve karaciğer sizrozu (KS) olgularındaki düzeyleri incelenerek, biyobelirteç olma potansiyellerinin belirlenmesi amaçlanmıştır. Gereç ve Yöntemler: Çalışmaya 60 hasta ve 26 sağlıklı gönüllü dahil edilmiştir. Serum örneklerinde total RNA izolasyonu, Direct-zolTM RNA MiniPrep (Zymo Research Corp., USA) ticari kiti ile yapıldıktan sonra cDNA sentezi ve gerçek zamanlı polimeraz zincir reaksiyonu amplifikasyonu EPIK miRNA Select Hi/Lo-ROX (Bioline Reagents Ltd., USA) ile gerçekleştirilmiştir. Amplifikasyon ve analizler için Rotor-Gene Q (QIAGEN, Germany) cihazı kullanılmış, istatistiksel analizler SPSS 21 (IBM, USA) programı ile yapılmıştır. Bulgular: Hsa-miRNA-21-3p ve hsa-miRNA-122-3p seviyeleri HCV ilişkili KS dışındaki hasta gruplarında 3-4 kat oranında artışlar göstermiş, HCV enfeksiyonlu hastalarda hsa-miRNA-29a-3p ekspresyonunda anlamlı azalmalar saptanmıştır (p

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Cite This Article

KÖROĞLU, M., TOKA, B., ASLAN, F. G., ALTINDİŞ, M., KÜÇÜKKARA, G. (2022). microRNA in Patients with Hepatitis B and Hepatitis C Virus Associated Hepatocellular Carcinoma and Cirrhosis. Viral Hepatitis Journal, Cilt: 25 - Sayı: 3, 113–116. https://ajindex.com/articles/646375

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