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Journals / Van Tıp Dergisi / 2018 / Cilt: 25 - Sayı: 2

Investigation of the Effect of Hyperforin and Hypericin on Inflammatory Response in RAW 264.7 Macrophages

Hiperforin ve Hiperisinin RAW 264.7 Makrofajında İnflamatuar Yanıt Üzerine Etkisinin İncelenmesi

Mehmet BERKÖZ
Oruc ALLAHVERDİYEV
Metin YILDIRIM
Pages
124–131
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

For thisreason, it is possible to say that hypericin and hyperforincan be used to reduce the inflammatory response.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: This study aims to determine the antiinflammatoryproperty of hypericin and hyperforin usingin vitro model.Materials and Methods: In this study, 0, 25, 50, 75 and100 nM hyperforin and 0, 2.5, 5, 7.5 and 10 μM hypericinwere treated to lipopolysaccharide (LPS) stimulated RAW264.7 macrophages.
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Key Findings & Data:We aimed to display the antiinflammatoryeffects of hypericin and hyperforin viameasuring prostaglandin E2 (PGE2) and nitric oxide(NO) production and cyclooxygenase-2 (COX-2) andinducible nitric oxide synthase (iNOS) gene expression inLPS induced RAW 264.7 macrophages.Results: Our study has shown that all appliedconcentration of hyperforin and hypericin decreasednitric oxide (NO) production and inducible nitric oxidesynthase (iNOS) and cyclooxygenase-2 (COX-2) geneexpression in RAW 264.7 macrophages, significantly.Similarly, all applied concentration of hyperforin andhypericin decreased prostaglandin E2 (PGE2) productionbut this decrease was statistically significant only ingroups treated with 75 and 100 nM hyperforin and 7.5and 10 μM hypericin.Conclusion: This indicates that hyperforin and hypericininhibited NO and PGE2 production and iNOS and COX-2 gene expression in RAW 264.7 macrophages in a dosedependentmanner.
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Conclusion & Impact:For thisreason, it is possible to say that hypericin and hyperforincan be used to reduce the inflammatory response.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: This study aims to determine the antiinflammatoryproperty of hypericin and hyperforin usingin vitro model.Materials and Methods: In this study, 0, 25, 50, 75 and100 nM hyperforin and 0, 2.5, 5, 7.5 and 10 μM hypericinwere treated to lipopolysaccharide (LPS) stimulated RAW264.7 macrophages. We aimed to display the antiinflammatoryeffects of hypericin and hyperforin viameasuring prostaglandin E2 (PGE2) and nitric oxide(NO) production and cyclooxygenase-2 (COX-2) andinducible nitric oxide synthase (iNOS) gene expression inLPS induced RAW 264.7 macrophages.Results: Our study has shown that all appliedconcentration of hyperforin and hypericin decreasednitric oxide (NO) production and inducible nitric oxidesynthase (iNOS) and cyclooxygenase-2 (COX-2) geneexpression in RAW 264.7 macrophages, significantly.Similarly, all applied concentration of hyperforin andhypericin decreased prostaglandin E2 (PGE2) productionbut this decrease was statistically significant only ingroups treated with 75 and 100 nM hyperforin and 7.5and 10 μM hypericin.Conclusion: This indicates that hyperforin and hypericininhibited NO and PGE2 production and iNOS and COX-2 gene expression in RAW 264.7 macrophages in a dosedependentmanner. Inhibition of NO and PGE2production by hyperforin and hypericin is a result of theinhibition of iNOS and COX-2 gene expression. For thisreason, it is possible to say that hypericin and hyperforincan be used to reduce the inflammatory response.

Özet

Amaç: Bu çalışma in vitro model kullanarak hiperisin ve hiperforinin anti-inflamatuvar özelliğini belirlemeyi amaçlamaktadır. Gereç ve Yöntem: Bu çalışmada, lipopolisakkarit (LPS) ile uyarılmış RAW 264.7 makrofajları, 0, 25, 50, 75 ve 100 nM hiperforin ve 0, 2,5, 5, 7,5 ve 10 μM hiperisin ile muamele edilmiştir. LPS ile indüklenmiş RAW 264.7 makrofajlarında, prostaglandin E2 (PGE2) ve nitrik oksit (NO) üretimi ile siklooksijenaz-2 (COX-2) ve indüklenebilir nitrik oksit sentaz ( iNOS) gen ekspresyonunu ölçerek hiperisin ve hiperforinin antienflamatuar etkilerini göstermeyi amaçladık. Bulgular: Yaptığımız çalışma, uygulanan tüm hiperforin ve hiperisin konsantrasyonlarının, RAW 264.7 makrofajlarında nitrik oksit (NO) üretimini ve indüklenebilir nitrik oksit sentaz ( iNOS) ve siklooksijenaz 2 (COX-2) gen ekspresyonlarını önemli ölçüde azalttığını göstermektedir. Benzer bir şekilde, uygulanan tüm hiperforin ve hiperisin konsantrasyonlarının prostaglandin E2 (PGE2) üretimini azalttığı ancak bu düşüşün sadece 75 ve 100 nm hiperforin ile 7,5 ve 10 μM hiperisin uygulanan gruplarda istatistiksel olarak anlamlı olduğu görülmüştür. Sonuç: Bu durum, hiperforin ve hiperisinin doza bağımlı bir şekilde RAW 264.7 makrofajlarındaki NO ve PGE 2 üretimini ve iNOS ve COX-2 gen ekspresyonunu inhibe ettiğini göstermektedir. Bu nedenle, hiperisin ve hiperforinin inflamatuar yanıtı azaltmak için kullanılabileceğini söylemek mümkündür.

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Cite This Article

BERKÖZ, M., ALLAHVERDİYEV, O., YILDIRIM, M. (2022). Investigation of the Effect of Hyperforin and Hypericin on Inflammatory Response in RAW 264.7 Macrophages. Van Tıp Dergisi, Cilt: 25 - Sayı: 2, 124–131. https://ajindex.com/articles/645995

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