SavedAdd your journal

Journals / Türk Osteoporoz Dergisi / 2020 / Cilt: 26 - Sayı: 2

The Relationship Between Postmenopausal Osteoporosis and Autophagic Gene Polymorphisms

Postmenopozal Osteoporoz ve Otofajik Gen Polimorfizmleri Arasındaki İlişki

Türkan TURGAY
Elif PALA
Ali AYDENİZ
Savaş GÜRSOY
Özlem ALTINDAĞ
Filiz ÖZBAŞ GERÇEKER
Pages
127–131
Publication Date
2022-11-06
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

However, no significant differencewere detected in genotype and allele frequencies in terms of these polymorphisms between the patient and the control groups (p>0.05).Conclusion: In summary, the results of our study do not support the hypothesis that ATG16L1 rs2241880, ATG10 rs1864183 and ATG5rs2245214 polymorphisms influence the predisposition for osteoporosis in postmenopausal women.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: This study investigates the role of ATG16L1 rs2241880, ATG10 rs1864183 and ATG5 rs2245214 gene polymorphisms, which areinvolved in autophagosome formation, in the susceptibility to postmenopausal osteoporosis (PMO) disease.Materials and Methods: Hundred PMO patients and 100 healthy controls without PMO were included into the study.
📊
Key Findings & Data:The distribution ofthe genotypes of ATG16L1 rs2241880, ATG10 rs1864183 and ATG5 rs2245214 polymorphisms in these subjects were analyzed using theTaqMan 5´-exonuclease allelic discrimination assay.Results: The T allele was detected more frequent among patients with osteoporosis (53%) of the ATG10 rs1864183 polymorphism.
💡
Conclusion & Impact:However, no significant differencewere detected in genotype and allele frequencies in terms of these polymorphisms between the patient and the control groups (p>0.05).Conclusion: In summary, the results of our study do not support the hypothesis that ATG16L1 rs2241880, ATG10 rs1864183 and ATG5rs2245214 polymorphisms influence the predisposition for osteoporosis in postmenopausal women.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: This study investigates the role of ATG16L1 rs2241880, ATG10 rs1864183 and ATG5 rs2245214 gene polymorphisms, which areinvolved in autophagosome formation, in the susceptibility to postmenopausal osteoporosis (PMO) disease.Materials and Methods: Hundred PMO patients and 100 healthy controls without PMO were included into the study. The distribution ofthe genotypes of ATG16L1 rs2241880, ATG10 rs1864183 and ATG5 rs2245214 polymorphisms in these subjects were analyzed using theTaqMan 5´-exonuclease allelic discrimination assay.Results: The T allele was detected more frequent among patients with osteoporosis (53%) of the ATG10 rs1864183 polymorphism. C allelein ATG16L1 rs2241880 polymorphism in the group of patients with osteoporosis was observed more frequent (56.5%). Besides, the G alleleof the ATG5 rs2245214 polymorphism was identified more common in PMO (34%) than in control group. However, no significant differencewere detected in genotype and allele frequencies in terms of these polymorphisms between the patient and the control groups (p>0.05).Conclusion: In summary, the results of our study do not support the hypothesis that ATG16L1 rs2241880, ATG10 rs1864183 and ATG5rs2245214 polymorphisms influence the predisposition for osteoporosis in postmenopausal women.

Özet

Amaç: Bu çalışmada otofajik mekanizmada otofagozom oluşumunda yer alan ATG16L1 rs2241880, ATG10 rs1864183 ve ATG5 rs2245214 gen polimorfizmlerinin postmenopozal osteoporoz (PMO) hastalığına yatkınlıktaki rolü incelenmiştir. Gereç ve Yöntem: Bu çalışmaya 50 yaş ve üzeri PMO’su olan 100 hasta ve PMO gelişimi olmayan 100 kontrol grubu alınmıştır. ATG16L1 rs2241880, ATG10 rs1864183 ve ATG5 rs2245214 polimorfizmlerinin genotiplendirmesi amacıyla polimorfik dizileri çoğaltmak için diziye özgü primerler ve her polimorfizmin her iki alelini saptamak için TaqMan 5´-ekzonükleaz alelik diskriminasyon yöntemi kullanılmıştır. Bulgular: ATG10 rs1864183 polimorfizminin osteoporozlu (%53) hastalarda T aleli daha sık saptanmıştır. Osteoporozlu hasta grubunda ATG16L1 rs2241880 polimorfizmindeki C aleli daha sık görülmüştür (%56,5). Ayrıca, ATG5 rs2245214 polimorfizminin G aleli, PMO’da (%34) kontrol grubuna göre daha yüksek olarak tanımlanmıştır. Ancak genotip ve alel frekanslarında hasta ve kontrol grupları arasındaki bu polimorfizmler açısından anlamlı fark saptanmamıştır (p>0,05). Sonuç: Özetle, çalışmamızdaki sonuçlar ATG16L1 rs2241880, ATG10 rs1864183 ve ATG5 rs2245214 polimorfizmlerinin postmenopozal kadınlarda osteoporoza yatkınlık sağlayabileceği hipotezini desteklememektedir.

🔖

Cite This Article

TURGAY, T., PALA, E., AYDENİZ, A., GÜRSOY, S., ALTINDAĞ, Ö., GERÇEKER, F. Ö. (2022). The Relationship Between Postmenopausal Osteoporosis and Autophagic Gene Polymorphisms. Türk Osteoporoz Dergisi, Cilt: 26 - Sayı: 2, 127–131. https://ajindex.com/articles/639628

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (17)

  1. Kanis JA, Melton LJ, Christiansen C, Johnston CC, Khaltaev N. The diagnosis of osteoporosis. J Bone Miner Res 1994:1137-41
  2. Hepgüler S, Cetin A, Değer C, Erkent U. Acta Orthop Traumatol Turc. Osteoporotic hip fracture costs in the elderly Turkish population 2011;45:316-25
  3. Farhat GN, Cauley JA. The link between osteoporosis and cardiovascular disease. Clin Cases Miner Bone Metab 2008;5:19- 34
  4. Tuzun S, Eskiyurt N, Akarirmak U, Saridogan M, Johansson H, McCloskey E, et al. The impact of a FRAX-based intervention threshold in Turkey: the FRAX-TURK study. Arch Osteoporos 2012;7:229-35
  5. Tuzun S, Eskiyurt N, Akarirmak U, Saridogan M, Senocak M, Johansson H, et al. Incidence of hip fracture and prevalence of osteoporosis in Turkey: the FRACTURK study. Osteoporos Int 2012;23:949-55
  6. Tella SH, Gallagher JC. Prevention and treatment of postmenopausal osteoporosis. J Steroid Biochem Mol Biol 2014;142:155-70
  7. Klein-Nulend J, van Oers RF, Bakker AD, Bacabac RG. Bone cell mechanosensitivity, estrogen deficiency, and osteoporosis. J Biomech 2015;48:855-65
  8. Raisz L, Seeman E. Causes of age related bone loss and bone fragility: an alternative view. J Bone Miner Res 2001;16:1948-52
  9. Demontiero O, Vidal C, Duque G. Aging and bone loss: new insights for the clinician. Ther Adv Musculoskelet Dis 2012;4:61- 76
  10. Hamrick I, Schrager S, Nye AM. Treatment of osteoporosis: current state of the art. Wien Med Wochenschr 2015;165:54-64
  11. Whittier X, Saag KG. Glucocorticoid-induced osteoporosis. Rheum Dis Clin North Am 2016;42:177-89
  12. Khosla S, Oursler MJ, Monroe DG. Estrogen and the skeleton. Trends Endocrinol Metab 2012;23:576-81
  13. Lunt M, Masaryk P, Nave CS, Nijs J, Poor G, Pols G, et al. The effects of lifestyle, dietary dairy intake, and diabetes bone density and vertebral deformity prevalance: The EVOS study. Osteoporos Int 2001;12:688-98
  14. Chen K, Yang YH, Jiang SD, Jiang LS. Decreased activity of osteocyte autophagy with aging may contribute to the bone loss in senile population. Histochem Cell Biol 2014;142:285-95
  15. Almeida M, O’Brien CA. Basic biology of skeletal aging: role of stress response pathways. J Gerontol A Biol Sci Med Sci 2013;68:1197-208
  16. Smith M, Wilkinson S. Essays Biochem. ER homeostasis and autophagy 2017;61:625-35
  17. Kim JH, Kang HM, Yu SB, Song JM, Kim CH, Kim BJ, et al. Cytoprotective effect of flavonoid-induced autophagy on bisphosphonate mediated cell death in osteoblast. J Cell Biochem 2018;119:5571-80