SavedAdd your journal

Journals / Türk Onkoloji Dergisi / 2008 / Cilt: 23 - Sayı: 3

Protective effect of N-acetyl cysteine on radiation-induced DNA damage in rat bone marrow: a comparison with WR-2721

N-asetil-sistein'in sıçan kemik iliği hücrelerinde radyasyona bağlı DNA hasarından koruyucu etkisi ve WR-2721 ile karşılaştırması

İzci Özlem AY
Can DEMİREL
Sevil KILÇIKSIZ
Nurten ERDAL
M. Ertan AY
Serkan GÜRGÜL
Pages
120–125
Publication Date
2022-11-05
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

R + NAC and R + WR-2721 groups showed significantly lower CA and higher MI averages when compared to R.CONCLUSION The results give clues about the beneficial effects of NAC against radiation-induced genocytotoxicity.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:OBJECTIVES To evaluate the potential radioprotective effects of N-acetylcysteine (NAC) against genocytotoxicity.
📊
Key Findings & Data:As representative of a clinically used radioprotector, the effect of WR-2721 was compared with that of NAC using chromosomal aberration (CA) and mitotic index (MI) in the irradiated rat's femoral bone marrow cells.
💡
Conclusion & Impact:R + NAC and R + WR-2721 groups showed significantly lower CA and higher MI averages when compared to R.CONCLUSION The results give clues about the beneficial effects of NAC against radiation-induced genocytotoxicity.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

OBJECTIVES To evaluate the potential radioprotective effects of N-acetylcysteine (NAC) against genocytotoxicity. As representative of a clinically used radioprotector, the effect of WR-2721 was compared with that of NAC using chromosomal aberration (CA) and mitotic index (MI) in the irradiated rat's femoral bone marrow cells. METHODS The rats (n=48) were divided randomly and equally into six groups as: Control (C), NAC (received 1000 mg/kg NAC), WR-2721 (200 mg/kg WR-2721), Radiation (R, received irradiation), R + NAC (received irradiation and 1000 mg/kg NAC), and R + WR-2721 (received irradiation and 200 mg/kg WR-2721). All the irradiated groups received whole-body gamma irradiation as a single dose of 6 Gy. At 72th hours, the rats were sacrificed and bone marrow cells were bilaterally collected from rats' femur. Then, cytogenetic and cytotoxicity tests were performed according to convantional methods.RESULTS Group R showed significantly higher CA and lower MI values when compared to C. R + NAC and R + WR-2721 groups showed significantly lower CA and higher MI averages when compared to R.CONCLUSION The results give clues about the beneficial effects of NAC against radiation-induced genocytotoxicity.

Özet

AMAÇ Deneysel çalışmamızda radyasyonun biyolojik sistemlerde neden olduğu genotoksik etkilere karşı, N-asetil-sisteinin (NAS) koruyucu etkileri konvensiyonel sitogenetik yöntemlerle (kromozom aberasyonu-CA, mitotik indeks-MI) amifostinle (WR-2721) karşılaştırıldı. GEREÇ VE YÖNTEM Co-60 cihazı ile 6 Gy tek doz tüm vücudu ışınlanan sıçanların femur-kemik ilikleri incelendi. Kontrol (K); radyasyon (R); NAS; amifostin (WR-2721) R + NAS (R + NAS); R + WR-2721 gruplarında, eşit sayıda toplam 48 dişi sıçan kullanıldı. R grubu yalnız radyasyon, R + WR-2721 grubuna radyasyon öncesi WR-2721 (200 mg/kg, i.p.), R + NAS grubuna NAS (1000 mg/kg, i.p.) verildi; 72 saat sonra tüm grupların preperasyonu konvensiyonel sitogenetik yöntemlerle hazırlanarak incelendi.BULGULAR R grubunun ortalama CA değeri en yüksek, MI değeri düşük olup; kontrol grubuna göre bu fark anlamlıydı. R + NAS ve R + WR-2721 gruplarında R grubuna göre hasar göstergeleri daha düşüktü; farklar istatistiksel olarak anlamlıydı.SONUÇ Bu sonuçlara göre, iyonize radyasyonun erken dönemdeki genotoksik etkilerine karşı amifostin ve NAS'nin koruyucu etkilerinin benzer olduğunu söylenebilir

🔖

Cite This Article

AY, İ. Ö., DEMİREL, C., KILÇIKSIZ, S., ERDAL, N., AY, M. E., GÜRGÜL, S. (2022). Protective effect of N-acetyl cysteine on radiation-induced DNA damage in rat bone marrow: a comparison with WR-2721. Türk Onkoloji Dergisi, Cilt: 23 - Sayı: 3, 120–125. https://ajindex.com/articles/639371

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (16)

  1. Görpe A, Cantez S. Pratik nükleer tıp. İstanbul: İstanbul Tıp Fakültesi Vakfı, Nobel Tip Kitabevi; 1992. s. 14-7
  2. Machlin LJ, Bendich A. Free radical tissue damage: protective role of antioxidant nutrients. FASEB J 1987;1(6):441-5
  3. Mc Clellan RO. The control of exposure of the public to ionizing radiation in the event of accident or attack. Biol effect of low-level rad 1983;288-301
  4. Macieira-Coelho A. Cancer and aging. Exp Gerontol 1986;21(6):483-95
  5. Holmes GE, Bernstein C, Bernstein H. Oxidative and other DNA damages as the basis of aging: a review. Mutat Res 1992;275(3-6):305-15
  6. Bohr VA, Dianov GL. Oxidative DNA damage processing in nuclear and mitochondrial DNA. Biochimie 1999;81(1-2):155-60
  7. El-Habit OH, Saada HN, Azab KS, Abdel-Rahman M, El-Malah DF. The modifying effect of beta-carotene on gamma radiation-induced elevation of oxidative reactions and genotoxicity in male rats. Mutat Res 2000;466(2):179-86
  8. Hui Z, Naikum Z, Rang Z, Xiumin L, Huifang C. Effect of ionizing radiation on bio-oxidase activities in cytoplasm of mouse blood liver cells. Chin J Radiol Med Prot 1996;16:179-82
  9. Khanna KK, Jackson SP. DNA double-strand breaks: signaling, repair and the cancer connection. Nat Genet 2001;27(3):247-54
  10. Au WW, Salama SA, Sierra-Torres CH. Functional characterization of polymorphisms in DNA repair genes using cytogenetic challenge assays. Environ Health Perspect 2003;111(15):1843-50
  11. Lucas JN, Awa A, Straume T, Poggensee M, Kodama Y, Nakano M, et al. Rapid translocation frequency analysis in humans decades after exposure to ionizing radiation. Int J Radiat Biol 1992;62(1):53-63
  12. Darroudi F, Natarajan AT, Bentvelzen AJ, Heidts PJ, Van Rotterdam A, Zoetelief J, et al. Detection of total and partial body irradiation in a mice model: a comparative study of chromosomal aberration, micronucleus and premature chromosome condensation assays. Int J Rad Biol 1992;74(2):207-15
  13. Sener G, Kabasakal L, Atasoy BM, Erzik C, Velioğlu-Oğünç A, Cetinel S, et al. Propylthiouracil-induced hypothyroidism protects ionizing radiation induced multiple organ damage in rats. J Endocrinol 2006;189(2):257-69
  14. Russell NS, Begg AC. Editorial radiotherapy and oncology 2002: predictive assays for normal tissue damage. Radiother Oncol 2002;64(2):125-9
  15. Sy D, Hugot S, Savoye C, Ruiz S, Charlier M, Spotheim-Maurizot M. Radioprotection of DNA by spermine: a molecular modelling approach. Int J Radiat Biol 1999;75(8):953-61
  16. Yuhas MJ,