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Journals / Türk Nöroloji Dergisi / 2010 / Cilt: 16 - Sayı: 1

Ratların siyatik sinir, spinal kord ve beyin sapında metotreksatın neden olduğu oksidatif strese karşı kafeik asit fenetil esterin koruyucu rolü

Ameliorating role of caffeic acid phenethyl ester (CAPE) against methotrexate-induced oxidative stress in the sciatic nerve, spinal cord and brain stem tissues of rats

Süleyman KUTLUHAN
Efkan UZ
Vedat Ali YÜREKLİ
Önder ŞAHİN
Hasan Rıfat KOYUNCUOĞLU
Ahmet SONGUR
Ertığrul UZAR
Serkan KILBAŞ
Mustafa YILMAZ
H. Ramazan YILMAZ
Pages
12–20
Publication Date
2022-11-05
DOI
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⚡ TL;DR(Too Long; Didn't Read)

CONCLUSION: These results reveal that MTX increases oxidative stress in the sciatic nerve, spinal cord and brainstem of rats and that CAPE has a preventive effect on the oxidative stress via its antioxidant capacity.

📌 3 Key Takeaways

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Objective & Hypothesis:OBJECTIVE: Methotrexate (MTX)-associated neurotoxicity is an important clinical problem in cancer patients, but the mechanisms of MTX-induced neurotoxicity are not yet known exactly.
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Key Findings & Data:The aims of this study were (1) to investigate the possible role of malondialdehyde (MDA), superoxide dismutase (SOD) enzyme, glutathione peroxidase (GSH-Px) and catalase (CAT) in the pathogenesis of MTX-induced neurotoxicity and (2) to determine whether there is a putative protective effect of caffeic acid phenethyl ester (CAPE) on MTX-induced neurotoxicity in the spinal cord, brainstem and sciatic nerve of rats.
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Conclusion & Impact:CONCLUSION: These results reveal that MTX increases oxidative stress in the sciatic nerve, spinal cord and brainstem of rats and that CAPE has a preventive effect on the oxidative stress via its antioxidant capacity.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

OBJECTIVE: Methotrexate (MTX)-associated neurotoxicity is an important clinical problem in cancer patients, but the mechanisms of MTX-induced neurotoxicity are not yet known exactly. The aims of this study were (1) to investigate the possible role of malondialdehyde (MDA), superoxide dismutase (SOD) enzyme, glutathione peroxidase (GSH-Px) and catalase (CAT) in the pathogenesis of MTX-induced neurotoxicity and (2) to determine whether there is a putative protective effect of caffeic acid phenethyl ester (CAPE) on MTX-induced neurotoxicity in the spinal cord, brainstem and sciatic nerve of rats. METHODS: A total of 19 adult Wistar male rats were divided into three experimental groups. Group I, control group; Group II, MTX-treated group; and Group III, MTX + CAPE-treated group. MTX was administered to the MTX and MTX + CAPE groups intraperitoneally (IP) with a single dose of 20 mg/kg on the second day of the experiment. CAPE was administered to the MTX + CAPE group IP with a dose of 10 μmol/kg for 7 days. RESULTS: In the sciatic nerve and spinal cord tissue, CAT and GSH-Px activities were increased in the MTX group in comparison with the control group. CAPE treatment with MTX significantly decreased CAT and GSH-Px activities in the neuronal tissues of rats in comparison with the MTX group. In the spinal cord and brainstem tissues, SOD activity in the MTX group was decreased in comparison with the control group, but in the sciatic nerve, there was no significant difference. In the spinal cord and brainstem of rats, SOD activity was increased in the CAPE + MTX group when compared with the MTX group. The level of MDA was higher in the MTX group than in the control group. CAPE administration with MTX injection caused a significant decrease in MDA level when compared with the MTX group. CONCLUSION: These results reveal that MTX increases oxidative stress in the sciatic nerve, spinal cord and brainstem of rats and that CAPE has a preventive effect on the oxidative stress via its antioxidant capacity.

Özet

AMAÇ: Kanser hastalarında metotreksata bağlı nörotoksisite önemli klinik bir problemdir. Fakat metotreksatın (MTX) neden olduğu nörotoksisitenin mekanizması tam olarak bilinmemektedir. Bu çalışmanın amaçları; MTX'e bağlı nörotoksisitenin patogenezinde ma-londialdehid (MDA), süperoksid dismutaz (SOD), glutatyon peroksidaz (GSH-Px) ve katalaz (CAT)'ın olası rolü ile ratların siyatik sinir, beyin sapı ve medulla spinalisinde MTX'e bağlı nörotoksisitesinde koruyucu etkisi olup olmadığını araştırmaktır. YÖNTEMLER: Toplam 19 adet Wistar erkek rat üç deney grubuna ayrıldı. Grup 1: Kontrol grubu, Grup 2: MTX alan grup, Grup 3: MTX ve kafeik asit fenetil ester (CAPE) alan grup. MTX ve MTX + CAPE gruplarına deneyin ikinci gününde MTX 20 mg/kg tek doz periton içine verildi. MTX CAPE grubuna CAPE 10 pmol/kg/gün intraperitoneal olarak 7 gün verildi. BULGULAR: MTX grubunda siyatik sinir ve spinal kord dokusunda kontrol grubu ile karşılaştırıldığında CAT ve GSH-Px aktivitelerinde artış bulundu. MTX + CAPE ile MTX grubu karşılaştırıldığında CAT ve GSH-Px aktivitelerinde azalma saptandı. MTX grupta spinal kord ve beyin sapı dokularında SOD aktivitesi kontrolle karşılaştırıldığında azalma saptanırken, siyatik sinirde anlamlı fark bulunmadı. Spinal kord ve beyin sapı dokularında SOD aktivitesi MTX + CAPE grubunda MTX grupla karşılaştırıldığında anlamlı derecede artış bulundu. MDA seviyesi MTX grupta kontrol grubuna göre istatistiksel olarak anlamlı derecede yüksekti. MTX + CAPE grubunda MDA seviyeleri MTX grubuna göre istatistiksel olarak anlamlı derecede düşük bulundu. SONUÇ: Bu sonuçlar MTX'in rat siyatik sinir, medulla spinalis ve beyin sapında oksidatif stresi artırdığını ve CAPE'nin antioksidan etkisi nedeniyle oksidatif strese karşı koruyucu etkisini gösterir.

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Cite This Article

KUTLUHAN, S., UZ, E., YÜREKLİ, V. A., ŞAHİN, Ö., KOYUNCUOĞLU, H. R., SONGUR, A., UZAR, E., KILBAŞ, S., YILMAZ, M., YILMAZ, H. R. (2022). Ratların siyatik sinir, spinal kord ve beyin sapında metotreksatın neden olduğu oksidatif strese karşı kafeik asit fenetil esterin koruyucu rolü. Türk Nöroloji Dergisi, Cilt: 16 - Sayı: 1, 12–20. https://ajindex.com/articles/636998

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