SavedAdd your journal

Journals / Türk Mikrobiyoloji Cemiyeti Dergisi / 2007 / Cilt: 37 - Sayı: 1

Evaluation of immunoblotting test results in patients with positive antinuclear antibody

Anti nükleer antikorların pozitif saptandığı hastalarda immunoblotting test sonuçlarının değerlendirilmesi

Nail HIZLI
Metin TÜRKER
İlhan AFŞAR
Aslı Gamze ŞENER
Ahmet VURAL
Pages
39–42
Publication Date
2022-11-05
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The autoantibody against SS-A/Ro-52 antigen was the most frequent autoantibody determined by the IB test.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Determination of autoantibodies by the indirect immunofluorescent (IIF) techniqe is the most preferred method for the diagnosis of connective tissue disorders.
📊
Key Findings & Data:A total of 2587 patients were evaluated for ANA positivity by the IIF technique.
💡
Conclusion & Impact:The autoantibody against SS-A/Ro-52 antigen was the most frequent autoantibody determined by the IB test.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Determination of autoantibodies by the indirect immunofluorescent (IIF) techniqe is the most preferred method for the diagnosis of connective tissue disorders. Differentiation of autoantibodies is the basis of correct diagnosis in otoimmun diseases. This method allows the extracted antigens (ENA) to be used for the differentiation of autoantibodies clearly. In our study, we evaluated the results of Immunoblotting (IB) test in patients with positive anti-nuclear-antibody (ANA) which was determined by the IIF technique during one year period retrospectively. A total of 2587 patients were evaluated for ANA positivity by the IIF technique. Of the 2587 patients, 215 were found to be positive for +2< ANA by the IIF method, and they were studied by the IB test. Of the 215 patients with ANA positivity, 171 (79.5%) were found to have at least one autoantibody, whereas 44 (20.4%) were found to have no autoantibody by the IB test. The autoantibody against SS-A/Ro-52 antigen was the most frequent autoantibody determined by the IB test.

Özet

Otoimmun bağ dokusu hastalıklarının tanısında indirekt immun floresan (IIF) tekniği ile otoantikorların tespiti en çok tercih edilen yöntemdir. Otoimmun hastalıklarda otoantikorların ayırımını yapmak doğru tanı için temel oluşturur. Ekstrakte edilen nükleer antijenlerin kullanılmasıyla geliştirilen testler, otoantikorların ayırımını daha net olarak yapmıştır. Çalışmamızda bir yıllık süreçte IIF testi ile anti nükleer antikoru (ANA) pozitif saptanan hastalarda immunoblotting (IB) testi ile yapılan çalışma sonuçları retrospektif olarak değerlendirilmiştir. Toplam 2587 hastada IIF ile ANA araştırması yapılmıştır. IIF ile 215 hastada +2< ANA pozitifliği saptanarak immunoblotting çalışılmıştır. IB testi ile 171 (%79.5) hastada en az bir antikor tespit edilmiştir, 44 ( %20.4 ) hastada IIF testinde antikor saptanırken IB testinde hiçbir antikor tespit edilememiştir. En fazla SS-A/Ro-52 antijenine karşı antikor saptanmıştır.

🔖

Cite This Article

HIZLI, N., TÜRKER, M., AFŞAR, İ., ŞENER, A. G., VURAL, A. (2022). Evaluation of immunoblotting test results in patients with positive antinuclear antibody. Türk Mikrobiyoloji Cemiyeti Dergisi, Cilt: 37 - Sayı: 1, 39–42. https://ajindex.com/articles/636020

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (10)

  1. Fritzler MJ, Wiik A, Fritzler ML, Barr SG. The use and abuse of commercial kits used to detect autoantibodies. Arthritis Res Ther. 2003; 5: 192-201
  2. Yumuk Z, Çalışkan fi, Gündefl S, Willke A. Anti-Nükleer antikorları n araştırılması ve saptanmas›nda kullanılan teknikler. Türk Mikrobiyol Cem Derg 2005; 35: 40-44
  3. Peene I, Meheus L, Veys EM, De Keyser F. Detection and identification of antinuclear antibodies (ANA) in a large and consecutive cohort of serum samples referred for ANA testing. Ann Rheum Dis 2001; 60: 1131-6
  4. Egner W. The use of laboratory tests in the diagnosis of SLE.J Clin Pathol 2000; 53: 424-32. 5. van Venrooij WJ, Charles P, Maini RN. The consensus workshops for the detection of autoantibodies to intracellular antigens in rheumatic diseases.J Immunol Meth 1991; 140: 181-9
  5. Berkem R, Gümüfl T, Karakoç E, Acar N. Antiniükleer antikorları n tespitinde indirekt immunfloresan antikor ve immunoblot assay yöntemlerinin karşılaştırılması. Türk Mikrobiyol Cem Derg 2004; 34: 124-130 7. Rosenberg AM, Semchuk KM, McDuffie HH, Ledingham DL, Cordeiro DM, Cessna AJ, Irvine DG, Senthilselvan A, Dosman JA. Prevalence of antinuclear antibodies in a rural population. J Toxicol Environ Health A 1999; 57: 225-36
  6. Kang I, Siperstein R, Quan T, Breitenstein ML. Utility of age, gender, ANA titer and pattern as predictors of anti-ENA and-dsDNA antibodies. Clin Rheumatol 2004; 23: 509-15
  7. Toker E, Yavuz S, Direskeneli H. Anti-Ro/SSA and anti- La/SSB autoantibodies in the tear fluid of patients with Sjogren's syndrome. Br J Ophthalmol 2004; 88: 3847
  8. Faria AC, Barcellos KS, Andrade LE. Longitudinal fluctuation of antibodies to extractable nuclear antigens in systemic lupus erythematosus. J Rheumatol 2005; 32: 1267-72 11. Haddouk S, Ben Ayed M, Baklouti S, Hachicha J, Bahloul Z, Masmoudi H. Autoantibodies in systemic lupus erythematosus: spectrum and clinical associations] Pathol Biol (Paris) 2005; 53: 311-7. (Abstract
  9. Eissfeller P, Sticherling M, Scholz D, Hennig K, Luttich T, Motz M, Kromminga A. Comparison of different test systems for simultaneous autoantibody detection in connective tissue diseases. Ann N Y Acad Sci 2005; 1050: 327-39
  10. Damoiseaux JG, Tervaert JW. From ANA to ENA: how to proceed? Autoimmun Rev 2006; 5: 10-7.