SavedAdd your journal

Journals / Türk Mikrobiyoloji Cemiyeti Dergisi / 2007 / Cilt: 37 - Sayı: 4

Detection of antimicrobial susceptibilities and beta-lactamase production of Moraxella catarrhalis strains isolated from various clinical samples by isoelectric focusing

Çeşitli klinik örneklerden izole edilen Moraxella catarrhalis suşlarının antibiyotiklere duyarlılıklarının ve beta-laktamaz üretimlerinin izoelektrik odaklama yöntemi ile saptanması

Nevriye GÖNÜLLÜ
Sinem ÖZDEMİR
Pages
196–200
Publication Date
2022-11-05
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Three (6%) of the M. catarrhalis strains showed no beta-lactamases. 41 (87.2%) and six (12.8%) of the BRO beta- lactamases were identified as BRO-1 and BRO-2, respectively.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, we aimed to determine the prevalence of antimicrobial susceptibilities of Moraxella catarrhalis strains and the identification of BRO beta-lactamases.
📊
Key Findings & Data:Our study included 50 M.catarrhalis strain isolated from various clinical samples (45 sputum, 2 throat smear, 1 nose leakage smear, 1 hemoculture, 1 endotracheal aspirate) in the Department of Microbiology and Clinical Microbiology of Cerrahpasa Faculty of Medicine.
💡
Conclusion & Impact:Three (6%) of the M. catarrhalis strains showed no beta-lactamases. 41 (87.2%) and six (12.8%) of the BRO beta- lactamases were identified as BRO-1 and BRO-2, respectively.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, we aimed to determine the prevalence of antimicrobial susceptibilities of Moraxella catarrhalis strains and the identification of BRO beta-lactamases. Our study included 50 M.catarrhalis strain isolated from various clinical samples (45 sputum, 2 throat smear, 1 nose leakage smear, 1 hemoculture, 1 endotracheal aspirate) in the Department of Microbiology and Clinical Microbiology of Cerrahpasa Faculty of Medicine. The antimicrobial susceptibilities of strains against ampiciline, cefuroxime, cefotaxime, ceftriaxone, erythromicine, clindamicine, tetracycline, gentamycin, ciprofloxacine were detected by the agar dilution method with the recommendations of Clinical and Laboratory Standarts Institute. Moreover, betalactamases of M.catarrhalis strains were typed by isoelectric focusing method. Beta-lactamases positivity was detected in 47 (94%) of the M.catarrhalis strains by nitrocephin disc test. Three (6%) of the M. catarrhalis strains showed no beta-lactamases. 41 (87.2%) and six (12.8%) of the BRO beta- lactamases were identified as BRO-1 and BRO-2, respectively.

Özet

Bu çalışmada, Moraxella catarrhalis kökenlerinin antibiyotiklere duyarlılık oranlarının saptanması ve BRO beta-laktamazların tanımlanması amaçlanmıştır. Çalışmamıza, Cerrahpaşa Tıp Fakültesi Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı'na gelen çeşitli klinik örneklerden (45 balgam, iki boğaz sürüntüsü, bir burun sürüntüsü, bir hemokültür ve bir endotrakeal aspirat) izole edilen toplam 50 M.catarrhalis kökeni dahil edilmiştir. Suşların ampisilin, sefuroksim, sefotaksim, seftriakson, eritromisin, klindamisin, tetrasiklin, gentamisin ve siprofloksasine karşı duyarlılıkları, Clinical and Laboratory Standarts Institute önerilerine göre agar dilüsyon yöntemi ile tespit edilmiştir. Beta-laktamaz enzimleri, izoelektrik odaklama yöntemi ile tiplendirilmiştir. M.catarrhalis suşlarının 47'sinde (%94), nitrosefin disk testi ile beta-laktamaz pozitifliği belirlenmiş; üç köken ise (%6) beta-laktamaz negatif bulunmuştur. Beta-laktamazların 41'i (%87.2) BRO-1, altısı (%12.8) BRO-2 olarak tiplendirilmiştir.

🔖

Cite This Article

GÖNÜLLÜ, N., ÖZDEMİR, S. (2022). Detection of antimicrobial susceptibilities and beta-lactamase production of Moraxella catarrhalis strains isolated from various clinical samples by isoelectric focusing. Türk Mikrobiyoloji Cemiyeti Dergisi, Cilt: 37 - Sayı: 4, 196–200. https://ajindex.com/articles/636013

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (14)

  1. Morgan MG, Mckenzie H, Enright MC, Bain M, Emmanuel FXS. Use of molecular methods to characterize M.catarrhalis strains in a suspected outbreak of nosocomial infection. Eur J Clin Microbiol Infect Dis 1992; 11:305-312
  2. Karalus R, Campagnari A. M.catarrhalis. A review of an important human mucosal pathogen. Microbes Infect 2000; 2: 547-559
  3. Wallace RJ, Steingrube VA, Nash DR, Hollis DG, Şanagan C, Brown BA, Labidi A, Weaver RE. BRO β-lactamases of B.catarrhalis and Moraxella subgenus Moraxella, including evidence for chromosomal β-lactamase transfer by conjugation in B.catarrhalis, M.nonliguefaciens, and M.lacunata. Antimicrob Agents Chemother 1989; 33: 1845-1854
  4. Enright MC, McKenzie H. Moraxella (Branhamella) catarrhalis- clinical and molecular aspects of a rediscovered pathogen. J Med Microbiol 1997; 46: 360-371
  5. Mc Gregor K, Chang BJ, Mee BJ, Riley TV. Moraxella catarrhalis. Clinical significance, antimicrobial susceptibility and BRO beta-lactamases. Eur J Clin Microbiol Infect Dis 1998; 7: 219-234
  6. Bootsma HJ, Dijk HV, Verhoef J, Şeer A, Mooi FT. Molecular characterization of the BRO beta-lactamase of Moraxella (Branhamella) catarrhalis. Antimicrob Agents Chemother 1996; 40: 966-972
  7. Hol C, Van Dijke EE, Verduin CM, Verhoef J, Van Dijk H. Experimental evidence for Moraxella-induced penicillin neutralization in pneumoccoccal pneumonia. J Infect Dis 1994; 170: 1613-1616
  8. Verduin CM, Hol C, Şeer A, Dijk H, Belkum A. Moraxella catarrhalis: From emerging to established pathogen. Clin Microbiol Rev 2002; 15: 125-144
  9. Farmer T, Reading C. β-lactamases of Branhamella catarrhalis and their inhibition by clavulanic acid. Antimicrob Agents Chemother 1982; 21: 506-508
  10. Fung CP, Yeo SF, Livermore DM. Susceptibility of Moraxella catarrhalis isolates to β-lactam antibiotics in relation to B-lactamase patterns. J Antimicrob Chemother 1994; 3:215-222
  11. Nash DR, Wallace RJ, Steingrube VA, Shurin PA. Isoelectric focusing of beta-lactamases from sputum and middle ear isolates of Branhamella catarrhalis recovered in the United States. Drugs 1986; 31(Suppl 3): 47- 53
  12. Philippon A, Riou JY, Guibourdenche M, Stolongo F. Detection, distribution and inhibition of Branhamalla catarrhalis β-lactamases. Drugs 1986; 31(Suppl 3): 64-69
  13. Christensen JJ, Keiding J, Suchomacher H, Bruun B. Recognition of a new Branhamella catarrhalis β-lactamase BRO- 3. J Antimicrob Chemother 1991; 28: 774-775
  14. Steingrube VA, Wallace RJ, Beaulieu D. A membr