Viral Agents Identified in Cerebrospinal Fluid Samples

Also normal, and higher CSF protein levels were found in 17 (48.6%) and 18 (51.4%) patients, respectively.Conclusion: Using PCR based molecular methods for the investigation of all possible viral agents in CSF samples will conceivably provide opportunity for early diagnosis and therapy of viral CNS infections.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Viral infections of central nervous system (CNS) are rapidly progressive, acute infections with high mortality and morbidity rates, and affect people of every age.

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Key Findings & Data:Material and Methods: The results of a total of 2849 CSF samples from 704 patients, sent to Akdeniz University Hospital Central Laboratory, Department of Microbiology between 2010-2014 with a preliminary diagnosis of viral CNS infection, were retrospectively analyzed.

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Conclusion & Impact:Also normal, and higher CSF protein levels were found in 17 (48.6%) and 18 (51.4%) patients, respectively.Conclusion: Using PCR based molecular methods for the investigation of all possible viral agents in CSF samples will conceivably provide opportunity for early diagnosis and therapy of viral CNS infections.

🔬Design:Research Article

📍Scope:2010-2014 Verileri

⚙️Variables:Domain Parameters

Abstract

Objective: Viral infections of central nervous system (CNS) are rapidly progressive, acute infections with high mortality and morbidity rates, and affect people of every age. Polymerase chain reaction (PCR), which is a nucleic acid amplification test (NAAT) is frequently used for diagnosing viral infections of CNS. The aim of this study is to retrospectively analyze the results of cerebrospinal fluid (CSF) samples, which were sent to Akdeniz University Medical Faculty Hospital, Central Laboratory, Department of Microbiology to be investigated for viral agents by PCR method. Material and Methods: The results of a total of 2849 CSF samples from 704 patients, sent to Akdeniz University Hospital Central Laboratory, Department of Microbiology between 2010-2014 with a preliminary diagnosis of viral CNS infection, were retrospectively analyzed. The CSF samples were tested by the real-time PCR method for adenovirus (AdV), cytomegalovirus (CMV), Epstein Barr Virus (EBV), enterovirus (EV) and herpes simplex viruses type 1 and type 2 (HSV- 1, HSV- 2). Also, protein and glucose levels in CSF samples were recorded.Results: Thirty eight CSF samples from 35 patients were found to be positive for viral infectious agents. EBV DNA, EV RNA, HSV-I DNA, AdV DNA, CMV DNA and HSV-2 DNA were detected in 3.6% (11/308), 1.8% (8/447), 1.7% (12/721), 1% (4/405), 0.4% (1/271) and 0.2% (2/697) of the samples respectively. HSV-I DNA and EBV DNA were detected in one patient simultaneously. The CSF glucose levels of the patients, who were found to be positive for viral infectious agents were within normal limits in 19 (54.2%), low in six (17.1%) and high in ten (28.6%)patients. Also normal, and higher CSF protein levels were found in 17 (48.6%) and 18 (51.4%) patients, respectively.Conclusion: Using PCR based molecular methods for the investigation of all possible viral agents in CSF samples will conceivably provide opportunity for early diagnosis and therapy of viral CNS infections.

Özet

Amaç: Viral etkenlere bağlı santral sinir sistemi enfeksiyonları her yaşta görülebilen, akut seyirli ve hızlı ilerleyen, mortalite ve morbiditesi yüksek enfeksiyonlardır. Tanıda, nükleik asit amplifikasyon test yöntemlerinden polimeraz zincir reaksiyonu (PZR) sıklıkla tercih edilmektedir. Bu çalışmanın amacı, Akdeniz Üniversitesi Tıp Fakültesi Hastanesi Merkez Laboratuvarı, Mikrobiyoloji Bölümü'ne viral etkenlerin PZR yöntemi ile araştırılması için gönderilen beyin omurilik sıvısı (BOS) örneklerinde elde edilen sonuçların retrospektif olarak değerlendirilmesidir.Gereç ve Yöntem: Akdeniz Üniversitesi Hastanesi Merkez Laboratuvarı, Mikrobiyoloji Bölümü'ne 2010-2014 yılları arasında viral SSS enfeksiyonu ön tanısı ile gönderilen 704 hastaya ait 2849 BOS örneğinin sonuçları retrospektif olarak değerlendirilmiştir. BOS örneklerinde adenovirus (AdV), sitomegalovirus (CMV), Epstein Barr virus (EBV), enterovirus (EV), herpes simplex virus tip 1 ve tip 2 (HSV-1, HSV-2) virusları gerçek zamanlı PZR yöntemiyle araştırılmıştır. BOS örneklerinde protein ve glukoz ölçüm değerleri kaydedilmiştir. Bulgular: Otuz beş hastaya ait 38 BOS örneğinde araştırılan viral etkenler pozitif bulunmuştur. BOS örneklerinde saptanan viral etkenlerin oranları; EBV DNA, EV RNA, HSV-I DNA, AdV DNA, CMV DNA ve HSV-2 DNA için sırasıyla %3.6 (11/308), %1.8 (8/447), %1.7 (12/721), %1 (4/405), %0.4 (1/271) ve %0.2 (2/697) olarak bulunmuştur. Bir hastada HSV-1 DNA ve EBV DNA birlikte saptanmıştır. BOS örneklerinde viral etken saptanan hastaların BOS glukoz düzeyi, 19 (%54.2) hastada normal sınırlarda, altı (%17.1) hastada düşük ve on (%28.6) hastada yüksek bulunmuştur. BOS protein düzeyi ise 17 (%48.6) hastada normal sınırlarda ve 18 (%51.4) hastada yüksek bulunmuştur. Sonuç: BOS örneklerinde PZR temelli moleküler yöntemler kullanılarak olası tüm viral etkenlerin araştırılmasının erken tanı ve tedaviye olanak sağlayacağı düşünülmektedir.

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Cite This Article

MUTLU, D., SARINOĞLU, R. C., BAYSAN, B. Ö., ÖĞÜNÇ, D., ÇOLAK, D., SAĞLIK, İ. (2022). Viral Agents Identified in Cerebrospinal Fluid Samples. Türk Mikrobiyoloji Cemiyeti Dergisi, Cilt: 46 - Sayı: 4, 152–158. https://ajindex.com/articles/635892

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