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Journals / Türk Mikrobiyoloji Cemiyeti Dergisi / 2018 / Cilt: 48 - Sayı: 1

Quantitative Determination of HBsAg Levels in Chronically Infected Patients with Hepatitis B Virus

Hepatit B Virüs ile Kronik Enfekte Hastalarda HBsAg Düzeyinin Kantitatif Olarak Belirlenmesi

Özlem KANDEMİR
Seda TEZCAN ÜLGER
Nuran DELİALİOĞLU
Havva Didem OVLA ÇELİKCAN
Pages
60–65
Publication Date
2022-11-05
DOI
—
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In addition, quantitative HBsAg levels in HBeAg positive patients were found to be significantly higher relative to HBeAg negative patients (p

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Serum HBV DNA levels is the most common sign for virus replication in diagnosis and follow-up of patients with chronic hepatitis B (CHB) infection and it has been prevalently used with serologic markers and aminotransferase (ALT) levels.
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Key Findings & Data:In this study, it was aimed to investigate the quantitative HBsAg levels and to compare it with HBV DNA level, ALT-AST levels and HBeAg in CHB patients.Material and Methods: HBV DNA levels of 91 patients with CHB were measured with real-time PCR method (Cobas TaqMan TM, Roche Laboratories, Germany).
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Conclusion & Impact:In addition, quantitative HBsAg levels in HBeAg positive patients were found to be significantly higher relative to HBeAg negative patients (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Serum HBV DNA levels is the most common sign for virus replication in diagnosis and follow-up of patients with chronic hepatitis B (CHB) infection and it has been prevalently used with serologic markers and aminotransferase (ALT) levels. In recent years, it has been shown that HBsAg levels are associated with HBV DNA and ALT levels. In this study, it was aimed to investigate the quantitative HBsAg levels and to compare it with HBV DNA level, ALT-AST levels and HBeAg in CHB patients.Material and Methods: HBV DNA levels of 91 patients with CHB were measured with real-time PCR method (Cobas TaqMan TM, Roche Laboratories, Germany). Quantitative HBsAg assays were performed with the ELISA method (Architect i2000 SR, Abbott Laboratories, Germany) within a range of 0.05-250 IU/mL.Results: The median HBV DNA level was determined as 1538.50 (19.00-64 501 284.00) and the median HBsAg was 2210.06 (0,05-46 192,95). The correlation between HBsAg and HBV DNA levels was found statistically significant (p=0.05). In addition, quantitative HBsAg levels in HBeAg positive patients were found to be significantly higher relative to HBeAg negative patients (p

Özet

Amaç: Kronik hepatit B (KHB) enfeksiyonunun tanı ve izlenmesinde serum HBV DNA düzeyi virus replikasyonunun en yaygın göstergesi olup, serolojik belirteçler ve alanin aminotransferaz (ALT) düzeyinin ölçümü ile birlikte yaygınlıkla kullanılmaktadır. Son yıllarda ise, kantitatif HBsAg düzeyleri ile HBV DNA ve ALT düzeylerinin ilişkili olduğu gösterilmiştir. Bu çalışmada, KHB hastalarında kantitatif HBsAg seviyelerinin HBV DNA, ALT-AST seviyeleri ve HBeAg ile karşılaştırılarak incelenmesi amaçlanmıştır.Gereç ve Yöntem: Çalışmaya dâhil edilen 91 KHB'li hastanın HBV DNA düzeyleri, gerçek zamanlı PCR yöntemi (Cobas TaqManTM 48, Roche, Almanya) ile araştırıldı. Kantitatif HBsAg ölçümü ELISA yöntemi (Architect i2000SR, Abbott, Almanya) ile 0.05-250 IU/mL limit aralığında gerçekleştirildi.Bulgular: Hastaların, HBV DNA seviyesinin ortancası 1538.50 (19.00-64501284.00), kantitatif HBsAg değerinin ortancası 2210.06 (0.05-46192.95) olup, HBsAg ölçüm değerleri ile HBV DNA düzeyleri arasındaki doğrusal korelasyon istatistiksel olarak anlamlıdır (p

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Cite This Article

KANDEMİR, Ö., ÜLGER, S. T., DELİALİOĞLU, N., ÇELİKCAN, H. D. O. (2022). Quantitative Determination of HBsAg Levels in Chronically Infected Patients with Hepatitis B Virus. Türk Mikrobiyoloji Cemiyeti Dergisi, Cilt: 48 - Sayı: 1, 60–65. https://ajindex.com/articles/635851

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