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Journals / Türk Mikrobiyoloji Cemiyeti Dergisi / 2021 / Cilt: 51 - Sayı: 4

Use of MALDI TOF-MS Method After Lysis Filtration for Rapid Identification of Yeast

Maya Mantarlarının Hızlı Tanımlanmasında Lizis Filtrasyon Sonrası MALDI TOF-MS Yönteminin Kullanımı

Süleyha Hilmioğlu Polat
Dilek Yeşim Metin
Sami Eren
Pages
421–427
Publication Date
2022-11-06
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Sixty-four Candida species were found to be 100% compatible with the standard methods and this method was found to be advantageous for at least 48 hours compared to MALDI-TOF MS method which required subculture and for at least 72 hours compared to Dalmau plaque method.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The early diagnosis of candidosis is very important in fungal infections and to reduce mortality rates, short-term and reliable methods are needed.
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Key Findings & Data:Method: 100 Candida isolated from positive blood cultures sent to Ege University, School of Medicine, Medical Microbiology laboratory, were studied with Dalmau plaque, MALDI-TOF MS and after lysis filtration methods.
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Conclusion & Impact:Sixty-four Candida species were found to be 100% compatible with the standard methods and this method was found to be advantageous for at least 48 hours compared to MALDI-TOF MS method which required subculture and for at least 72 hours compared to Dalmau plaque method.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The early diagnosis of candidosis is very important in fungal infections and to reduce mortality rates, short-term and reliable methods are needed. In this study, the appropriateness of MALDI-TOF MS method after lysis filtration was investigated for the faster identification of Candida spp. Method: 100 Candida isolated from positive blood cultures sent to Ege University, School of Medicine, Medical Microbiology laboratory, were studied with Dalmau plaque, MALDI-TOF MS and after lysis filtration methods. The results were compared with those obtained from the classical MALDI-TOF MS method. Results: According to conventional and MALDI-TOF MS identification methods; 37 Candida albicans, 23 Candida parapsilosis, 17 Candida tropicalis, nine Candida glabrata, six Candida kefyr, three Candida guilliermondii, three Candida dubliniensis and three Candida krusei were identified. After lysis filtration method; 26 C. albicans, nine C. parapsilosis, 13 C. tropicalis, nine C. glabrata, two C. kefyr, three C. dubliniensis, two C. krusei. MALDI-TOF MS method and Dalmau plaque and after lysis filtration method were found to be compatible in 64 strains. Incompatibility was not due to incorrect identification, but spectrum deficiency. Conclusion: The strains, which are not identified as “spectrum deficiency” consist of the species in the database. Sixty-four Candida species were found to be 100% compatible with the standard methods and this method was found to be advantageous for at least 48 hours compared to MALDI-TOF MS method which required subculture and for at least 72 hours compared to Dalmau plaque method.

Özet

Amaç: İnvazif kandidozda erken tanı çok önemlidir ve mortalite oranını düşürmek için kısa sürede ve güvenilir sonuç veren yöntemlere ihtiyaç duyulmaktadır. Bu çalışmada, Candida türlerinin daha hızlı tanımlanabilmesi için lizis filtrasyon sonrası MALDI-TOF MS yönteminin uygunluğu araştırılmıştır. Yöntem: Bu çalışmada, Ege Üniversitesi, Tıp Fakültesi, Tıbbi Mikrobiyoloji Laboratuvarına gönderilen kan kültürlerinden izole edilen 100 Candida kökeni, Dalmau plak, MALDI-TOF MS ve lizis filtrasyon sonrası MALDI-TOF MS yöntemleriyle araştırıldı ve elde edilen sonuçlar karşılaştırıldı. Bulgular: Çalışmamızda konvansiyonel ve MALDI-TOF MS tanımlama yöntemlerine göre; 37 Candida albicans, 23 Candida parapsilosis, 17 Candida tropicalis, dokuz Candida glabrata, altı Candida kefyr, üç Candida guilliermondii, üç Candida dubliniensis, üç Candida krusei olarak identifiye edilmiştir. Lizis filtrasyon sonrası MALDI-TOF MS yönteminde ise identifikasyon sonuçları; 26 C. albicans, dokuz C. parapsilosis, 13 C. tropicalis, dokuz C. glabrata, iki C. kefyr, üç C. dubliniensis, iki C. krusei olarak belirlenmiştir. MALDI-TOF MS yöntemi ve Dalmau plak ile lizis filtrasyon sonrası MALDI-TOF MS yöntemi 64 kökende uyumlu bulunmuştur. Uyumsuzluğun yanlış tanımlamadan değil, spektrum yetersizliğinden kaynaklandığı gözlemlenmiştir. Sonuç: “Spektrum yetersizliği” olarak tanımlanmayan suşlar veri tabanında yer alan türlerden oluşmaktadır. Altmış dört Candida türünün standart yöntemlerle %100 uyumlu olacak şekilde tanımlama yaptığı ve bu yöntemin subkültür gerektiren MALDI-TOF MS yöntemine göre en az 48 saat, Dalmau plak yöntemine göre de en az 72 saat avantaj sağladığı görülmüştür.

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Cite This Article

Polat, S. H., Metin, D. Y., Eren, S. (2022). Use of MALDI TOF-MS Method After Lysis Filtration for Rapid Identification of Yeast. Türk Mikrobiyoloji Cemiyeti Dergisi, Cilt: 51 - Sayı: 4, 421–427. https://ajindex.com/articles/635754

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