Determination of CA15-3, CA 19-9, CA 125 and CEA Reference Intervals

Objective & Hypothesis:Objective: In this study we aimed to calculate the reference intervals of carbohydrate antigen 15-3 (CA15-3), carbohydrate antigen 19-9 (CA19-9), carbohydrate antigen 125 (CA125) and carsinoembriyogenic antigen (CEA).

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Key Findings & Data:Materials and Methods: 325 healthy individuals admitted to our hospital s outpatient units between 17.08.2009 - 11.09.2009 were included in the study according to Clinical and Laboratory Standarts Institute, CLSI Approved Guideline C28-A.

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Conclusion & Impact:We think that these values reflect our population more truly than manufacturers and should be used as reference intervals.

🔬Design:Research Article

📍Scope:2009 Verileri

⚙️Variables:Domain Parameters

Abstract

Objective: In this study we aimed to calculate the reference intervals of carbohydrate antigen 15-3 (CA15-3), carbohydrate antigen 19-9 (CA19-9), carbohydrate antigen 125 (CA125) and carsinoembriyogenic antigen (CEA). Materials and Methods: 325 healthy individuals admitted to our hospital s outpatient units between 17.08.2009 - 11.09.2009 were included in the study according to Clinical and Laboratory Standarts Institute, CLSI Approved Guideline C28-A. Serum levels of CA 15-3, CA19-9, CA125 and CEA were analyzed using Immulite 2000 System (Healthcare Diagnostics Products Ltd. England), a two-site chemiluminescent enzyme immunometric assay. Results: The mean CA 15-3 level was 22.32 ± 13.45 U/mL, mean CA19-9 level was 8.95 ± 9.10 U/mL, mean CA125 level was 7.97 ± 4.62 U/mL, mean CEA level was 2.45 ± 1.74 ng/mL. CA15-3 reference intervals were found as 8.33- 52.47 U/mL, CA19-9 reference intervals were found as 2.5-32.35 U/mL, CA125 reference intervals were found as 2.28-19.15 U/mL, CEA reference intervals were found as 0.36-6.09 ng/mL for 50 years of age. Conclusion: The reference values found in our study were different from the values recommended by the manufacturer. We think that these values reflect our population more truly than manufacturers and should be used as reference intervals.

Özet

Amaç: Bu çalışmada hastanemiz laboratuva rında çalışılan parametrelerden karbonhidrat antijen 15-3 (CA15-3), karbonhidrat antijen 19-9 (CA19-9), karbonhidrat antijen 125 (CA125) ve karsinoembriyojenik antijene (CEA) ait referans aralıklarının hesaplanması amaçlandı. Gereç ve Yöntem: 17.08.2009-11.09.2009 tarihleri arasında Dr. Lütfi Kırdar Kartal Eğitim ve Araştırma Hastanesi polikliniklerine başvuranlardan Klinik ve Laboratuvar Standartları Enstitüsü (Clinical and Laboratory Standarts Institute, CLSI) C28-A prosedürüne uygun olarak 325 birey seçildi. CA15-3, CA19-9, CA125 ve CEA düzeyleri Immulite 2000 otoanalizöründe (Healthcare Diagnostics Products Ltd., ngiltere) serumda solid faz two-site kemiluminesan enzim immün ölçüm yöntemiyle çalışıldı. Bulgular: Ortalama CA15-3 düzeyi 22.32 ± 13.45 U/ml, ortalama CA19-9 düzeyi 8.95 ± 9.10 U/mL, ortalama CA125 düzeyi 7.97 ± 4.62 U/mL, ortalama CEA düzeyi 2.45 ± 1.74 ng/mL olarak saptandı. CA15-3 referans aralığı 8.33-52.47 U/mL, CA19-9 referans aralığı 2.5-32.35 U/mL, CA125 referans aralığı 2.28-19.15 U/mL, CEA referans aralığı ise 50 yaş altı için 0.36-6.47 ve 50 yaş üstü için 1.01- 8.72 ng/mL olarak bulundu. Sonuç: Çalışma sonucunda elde edilen değerler üretici firmanın önerdiği değerlerden farklı bulundu. Elde edilen değerlerin içinde bulunduğumuz toplumun değerlerini daha gerçekçi yansıttığı ve laboratuvarda referans aralığı olarak kullanılabileceği düşünüldü.

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Cite This Article

YÜCEL, N., ORÇUN, A., ERCAN, Ş., TEZCAN, S., ÇAKIR, Ö. M. (2022). Determination of CA15-3, CA 19-9, CA 125 and CEA Reference Intervals. Türk Klinik Biyokimya Dergisi, Cilt: 11 - Sayı: 1, 15–20. https://ajindex.com/articles/634471

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