Journals / Türkiye Entomoloji Dergisi / 2015 / Cilt: 39 - Sayı: 4

Güney İran'da Aphis gossypii(Glover) (Hemiptera: Aphididae)'de imidacloprid direnç karakterizasyonu

Characterizationofimidacloprid resistance in Aphis gossypii(Glover) (Hemiptera: Aphididae)in Southern Iran

Pages
413–423
Publication Date
2022-11-05
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Combination of in vivo differential synergism studies and biochemical assays (esterase, GSTs, cytochrome P450 monooxygenase and heme peroxidase assay) suggest that the enhanced cytochrome P450 activity is the primary mechanism of increased resistance in these populations

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The cotton aphid, Aphis gossypii (Glover) (Hemiptera: Aphididae), is a key pest of cucurbits in Fars Province, Southern Iran and is managed with repetitive applications of insecticides such as imidacloprid.
📊
Key Findings & Data:Recently, reports of insecticide control failures have increased, particularly with imidacloprid.
💡
Conclusion & Impact:Combination of in vivo differential synergism studies and biochemical assays (esterase, GSTs, cytochrome P450 monooxygenase and heme peroxidase assay) suggest that the enhanced cytochrome P450 activity is the primary mechanism of increased resistance in these populations
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The cotton aphid, Aphis gossypii (Glover) (Hemiptera: Aphididae), is a key pest of cucurbits in Fars Province, Southern Iran and is managed with repetitive applications of insecticides such as imidacloprid. Recently, reports of insecticide control failures have increased, particularly with imidacloprid. In present work susceptibility of two A. gossypii populations to imidacloprid and effects of possible synergist, Triphenyl phosphate (TPP), Diethyl maleate (DEM) and Piperonyl butoxide (PBO) were checked using micro-applicator bioassay. The resistant population was collected from cucumber host plant in greenhouse and susceptible population had been reared under greenhouse conditions on Cucumis sativus L. cv. Negin (Cucurbitaceae) for two years. A resistance to imidacloprid with resistance ratio of 11.24 was found in resistant population compare with the susceptible population. Combination of in vivo differential synergism studies and biochemical assays (esterase, GSTs, cytochrome P450 monooxygenase and heme peroxidase assay) suggest that the enhanced cytochrome P450 activity is the primary mechanism of increased resistance in these populations

Özet

Pamuk yaprak biti Aphis gossypii (Glover) (Hemiptera: Aphididae), güney İran'ın Fars bölgesinde cucurbitlerin anahtar zararlılarından biridir ve imidaclorpid gibi tekrarlanarak kullanılan insektisitler ile kontrol edilmektedir. Son yıllarda insektisitlerinözelliklede imidaclorpid uygulamalarının başarısız olduğu kayıt edilmektedir. Bu çalışmada A. gossypii'nin iki duyarlı popülasyonunun imidaclorpidlere duyarlılığının ve Triphenyl phosphate (TPP), Diethyl maleate (DEM) vePiperonyl butoxide (PBO) gibi bazı sinerjist etkili kimyasalların imidaclorpit duyarlılğına etkisi mikro aplikatör kullanılan denemeler ile test edilmiştir. Yaprakbitinin dayanıklı popülasyonu seralardan kabakgiller üzerinden toplanmış, duyarlı popülasyon ise Cucumis sativusL. cv. Negin (Cucurbitaceae) üzerinde iki yıl süre ile yetiştirilmiştir. Imidoclorpid dayanıklılığı duyarlı popülasyona oranla 11.24 kat daha fazla bulunmuştur. Farklı sinerjik etkili maddelerin (esteraz, GSTs, cytochrome P450monooxygenazveheme peroxidaz) kombinasyonlarının test edildiği biyokimyasalçalışmalar sonucunda cytochrome P450mekanizmasının dayanıklılığı arttıran birincil mekanizma olduğu tespit edilmiştir.

🔖

Cite This Article

SEYEDEBRAHIMI, S. S., JAHROMI, K. T., IMANI, S., NAVEH, V. H., HESAMI, S. (2022). Güney İran'da Aphis gossypii(Glover) (Hemiptera: Aphididae)'de imidacloprid direnç karakterizasyonu. Türkiye Entomoloji Dergisi, Cilt: 39 - Sayı: 4, 413–423. https://ajindex.com/articles/628089

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (7)

  1. Ahmad, M., M. I. Arif & I. Denholm, 2003. High resistance of field populations of the cotton aphid Aphis gossypii Glover (Homoptera: Aphididae) to pyrethroid insecticides in Pakistan. Journal of Economic Entomology, 96: 875- 878. Bradford, M. M., 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of proteindye binding. Analytical Biochemistry, 7: 248-254.
  2. Cabrera-Brandt, M.A., E. Fuentes-Contreras & C. C. Figueroa, 2010. Differences in the detoxification metabolism between two clonal lineages of the aphid Myzus persicae (Sulzer) (Hemiptera: Aphididae) reared on tobacco (Nicotiana tabacum L.). Chilean Journal of Agricultural Research, 70: 567-575. Castañeda, L.E., C.C. Figueroa, E. Fuentes-Contreras, H. M. Niemeyer & R. F. Nespolo, 2009. Energetic costs of detoxification systems in herbivores feeding on chemically defended host plants: a correlational study in the grain aphid, Sitobion avenae. Journal of Experimental Biology, 212: 1185-1190.
  3. Castaneda, L.E., C.C. Figueroa & R.F. Nespolo, 2010. Do insect pests perform better on highly defended plants? Costs and benefits of induced detoxification defences in the aphid Sitobion avenae. Journal of Evolutionary Biology, 23: 2474-2483.
  4. Choi, B.R., S. W. Lee & J. K. Yoo, 2001. Resistance mechanisms of green peach aphid, Myzus persicae to imidacloprid. Korean-Journal of Applied Entomology, 40: 265- 271. Daborn, P., S. Boundy, J. Yen, B. Pittendrigh & R. French-Constant, 2001. DDT resistance in Drosophila correlates with Cyp6g1 over-expression and confers cross-resistance to the neonicotinoid imidacloprid. Molecular Genetics and Genomics, 266: 556-563.
  5. Damayanthi, B. T. & S. H. P. P. Karunaratne, 2005. Biochemical characterization of insecticide resistance in insect pests of vegetables and predatory ladybird beetles. Journal of National Science Foundation of Sri Lanka, 33: 115- 122. De Sousa, G., A. Cuany, A. Brun, M. Amichot, R. Rahmani & J.Berge, 1995. A microfluorometric method for measuring ethoxycoumarin-O-deethylase activity on individual Drosophila melanogaster abdomens: interest for screening resistance in insect populations. Analytical Biochemistry, 229: 86-91.
  6. Ebert, T. A. & B. Cartwright, 1997. Biology and ecology of Aphis gossypii Glover (Homoptera: Aphididae). Southwestern Entomologist, 22: 115-153.
  7. El-Kady, H., 2007. Insecticide resistance in the cotton aphid, Aphis gossypii Glover in Egypt. Journal of Egyptian Society of Toxicology, 36: