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Journals / Türk Hijyen ve Deneysel Biyoloji Dergisi / 2022 / Cilt: 79 - Sayı: 1

Evaluation of real-time PCR cycle threshold value, clinical and epidemiological characteristics of SARS-CoV-2 positive healthcare workers

SARS-CoV-2 pozitif sağlık çalışanlarının gerçek zamanlı PCR döngü eşik değeri, klinik ve epidemiyolojik özelliklerinin değerlendirilmesi

Gül BAYRAM
Harun GÜLBUDAK
Aslıhan BEKCİ
Seda TEZCAN ÜLGER
Gülçin YAPICI
Gönül ASLAN
Pages
13–24
Publication Date
2022-11-06
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The median RT-qPCR Ct value was determined as 23.17(19.3-29.4) and Ct value was found to be low (

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: During the new coronavirus infectious disease (COVID-19) pandemic, healthcare workers are the occupational group most affected by SARS-CoV-2.
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Key Findings & Data:Infection of healthcare workers with SARS-CoV-2 poses a potential risk of infection for other healthcare workers, patients and their families.
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Conclusion & Impact:The median RT-qPCR Ct value was determined as 23.17(19.3-29.4) and Ct value was found to be low (
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: During the new coronavirus infectious disease (COVID-19) pandemic, healthcare workers are the occupational group most affected by SARS-CoV-2. Infection of healthcare workers with SARS-CoV-2 poses a potential risk of infection for other healthcare workers, patients and their families. The viral load measure in the sample is determined as the cycle threshold (Ct) with the RT-qPCR method. It has been reported that the RT-qPCR Ct value may be related to the severity and potential contagiousness of the infection. In this study, it was aimed to evaluate the relationships between socio- demographic factors, COVID-19 symptoms and Ct value in SARS-CoV-2 RT-qPCR positive healthcare workers. Methods: In this study, 300 healthcare workers with positive SARS-CoV-2 RT-qPCR test were included. SARS- CoV-2 RT-qPCR test was performed from nasopharyngeal and oropharyngeal swab samples with Bio-speedy SARS-CoV-2 RT-qPCR (Bioeksen, Turkey) kit. The RT- qPCR Ct value results of healthcare staff patients were classified as low, medium and high, socio-demographic characteristics and the risk of viral spread was evaluated. Results: The rate of those who showed at least one symptom among the 300 health personnel included in the study was 88.3% (n=265) and the most common symptoms were muscle-joint pain 39.7%, fatigue 33% and sore throat 30.7%. The median RT-qPCR Ct value was determined as 23.17(19.3-29.4) and Ct value was found to be low (

Özet

Amaç: Sağlık çalışanları, yeni tip koronavirüs hastalığı (COVID-19) pandemisi sürecinde SARS-CoV- 2’den en fazla etkilenen meslek grubudur. Sağlık çalışanlarının SARS-CoV-2 ile enfekte olması, diğer sağlık çalışanları, hastalar ve aileleri için enfeksiyon riski oluşturmaktadır. RT-qPCR yöntemi ile örnekteki viral yük ölçüsü döngü eşik değeri “cycle threshold (Ct)” olarak saptanmaktadır. RT-qPCR Ct değerinin, enfeksiyonun şiddeti ve bulaştırıcılığı ile ilişkili olabileceği bildirilmiştir. Bu çalışmada SARS-CoV-2 RT- qPCR testi pozitif bulunan sağlık çalışanlarında sosyo- demografik faktörler, COVID-19 semptomları ve Ct değeri arasındaki ilişkilerin incelenmesi amaçlanmıştır. Yöntem: Çalışmaya SARS-CoV-2 RT-qPCR testi pozitif bulunan 300 sağlık çalışanı dahil edilmiştir. SARS- CoV-2 RT-qPCR testi, nazofarengeal ve orofarengeal sürüntü örneklerinden Bio-speedy SARS-CoV-2 RT- qPCR (Bioeksen, Türkiye) kiti ile yapılmıştır. Sağlık çalışanlarının RT-qPCR Ct değeri sonuçları düşük, orta ve yüksek olarak sınıflandırılarak viral yayılım riski ve sosyo-demografik özellikleri değerlendirilmiştir.

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Cite This Article

BAYRAM, G., GÜLBUDAK, H., BEKCİ, A., ÜLGER, S. T., YAPICI, G., ASLAN, G. (2022). Evaluation of real-time PCR cycle threshold value, clinical and epidemiological characteristics of SARS-CoV-2 positive healthcare workers. Türk Hijyen ve Deneysel Biyoloji Dergisi, Cilt: 79 - Sayı: 1, 13–24. https://ajindex.com/articles/624921

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