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Journals / Türk Biyokimya Dergisi / 2013 / Cilt: 38 - Sayı: 4

A case of Hemoglobin Beckman; An example of hemoglobin variants interfering with HbA1c measurement

Hemoglobin Beckman olgusu; HbA1c ölçümünü interfere eden hemoglobin varyantlarına bir örnek

Nihal YÜCEL
Özlem Çakır MADENCİ
Lale DAĞDELEN
Derya AKSOY
Asuman Orçun KAPTANAĞASI
Pages
369–371
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: This new case of Hb Beckman is thought to be a modest contribution to the subject of abnormal hemoglobins that interfere the HbA1c measurement

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: It has been known for a long time that the presence of clinically silent hemoglobin (Hb) variants in blood samples could influence the measurement of glycohemoglobin (HbA1c) since these abnormal hemoglobins might interfere with some methodologies.
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Key Findings & Data:Material and Methods: The variant was detected during the HbA1c measurement by cation exchange high performance chromatography (CE-HPLC) in a patient with diabetes mellitus.
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Conclusion & Impact:Conclusion: This new case of Hb Beckman is thought to be a modest contribution to the subject of abnormal hemoglobins that interfere the HbA1c measurement
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: It has been known for a long time that the presence of clinically silent hemoglobin (Hb) variants in blood samples could influence the measurement of glycohemoglobin (HbA1c) since these abnormal hemoglobins might interfere with some methodologies. In the present study we describe the first case of Hb Beckman in Turkey. Material and Methods: The variant was detected during the HbA1c measurement by cation exchange high performance chromatography (CE-HPLC) in a patient with diabetes mellitus. Since a HbA1c result could not be obtained by this method, the test was repeated by boronate affinity HPLC. Results: Further investigation performed in order to identify the variant revealed heterozygote Hb Beckman 135 GCT-GAT (Ala --> Asp) which explained the inappropriate HbA1c result by CE-HPLC. Conclusion: This new case of Hb Beckman is thought to be a modest contribution to the subject of abnormal hemoglobins that interfere the HbA1c measurement

Özet

Amaç: Klinik belirti vermeyen hemoglobin (Hb) varyantlarının glikohemoglobin (HbA1c) ölçümlerini etkileyebildiği uzun zamandan beri bilinmektedir; zira bu varyantlar bazı ölçüm yöntemleri ile interferans göstermektedir. Bu makalede Türkiye'deki ilk Hemoglobin (Hb) Beckman vakası sunulmaktadır. Gereç ve Yöntemler: Varyant, diyabetli bir hastada katyon değişimli yüksek performans sıvı kromatografisi (CE-HPLC) ile HbA1c ölçümü sırasında tespit edilmiştir. Bu yöntemle HbA1c sonucu elde edilemediğinden ölçüm boronat afinite HPLC yöntemi ile tekrarlanmıştır. Bulgular: Varyantı tanımlamak için yapılan ileri tetkiklerde heterozigot Hb Beckman 135 GCT-GAT (Ala --> Asp) tespit edilmiş ve böylece CE-HPLC ile HbA1c sonucu elde edilememesinin nedeni açıklığa kavuşmuştur. Sonuçlar: Bu yeni Hb Beckman vakasının HbA1c ölçümleri ile interferans yapan anormal hemoglobinlere mütevazı bir katkı olduğu düşünülmektedir

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Cite This Article

YÜCEL, N., MADENCİ, Ö. Ç., DAĞDELEN, L., AKSOY, D., KAPTANAĞASI, A. O. (2022). A case of Hemoglobin Beckman; An example of hemoglobin variants interfering with HbA1c measurement. Türk Biyokimya Dergisi, Cilt: 38 - Sayı: 4, 369–371. https://ajindex.com/articles/621430

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References (6)

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