AI Summary & Key Takeaways
AI InsightThe fermentation profile and kinetics study indicate the superiority of the mutant strain for n-butanol production from blackstrap molasses.
📌 3 Key Takeaways
Abstract
The present study deals with the enhancement of n-butanol production by a mutant strain of Clostridium acetobutylicum using blackstrap molasses as substrate (a by product of sugar industries). The bacteria were cultured in 14 L glass stainless steel fermentor. The parent strain Cl. acetobutylicum PTCC-23 was mutagenized for the improved production of butanol by UV exposure, N-methyl-N-nitro-N-nitrosoguanidine and ethyl methane sulphonate treatments. After extensive screening and optimization, a total of 15 different mutants were selected. The best butanol producing strain was designated as MEMS-7, which yielded 20 % more butanol compared to parent strain after 91 h of incubation at 30oC. The optimum sugar level in molasses medium was 60.0 g/L. The growth of the parent strain PTCC-23 was inhibited at 1.6 mol/L butanol. The butanol tolerance of the mutant strain MEMS-7 on the other hand was found to be 3.84 mol/L. The fermentation profile and kinetics study indicate the superiority of the mutant strain for n-butanol production from blackstrap molasses.
Özet
Bu çalışma şeker endüstrisinin yan ürünü olan melası substrat olarak kullanan mutant Clostridium acetobutylicum suşunun n-butanol üretimindeki artışla ilgilidir. Bakteri 14 L cam paslanmaz çelik fermentörde üretildi. Cl. acetobutylicum PTCC- 23 butanol üretimini arttırmak için UV ışığı, N-metil-N-nitro-N-nitrosoguanidin ve etil metan sulfonat ile muamele edildi. Yoğun tarama ve optimizasyon çalışmaları sonucu 15 farklı mutant seçildi. En iyi butanol üreten MEMS-7 suşu, 30 oC’da 91 saat inkübasyondan sonra % 20 fazla butanol üretti. Melaslı ortamın optimum şeker düzeyi 60.0 g/L idi. Ana suş PTCC-23’ün büyümesi 1.6 mol /L butanol tarafından inhibe edildi. Diğer yandan mutant suş MEMS-7’nin butanol toleransı 3.84 mol/L olarak bulundu. Fermentasyon profile ve kinetic çalışmalar mutant suşun melastan n-butanol üretimindeki üstünlüklerini gösterdi.