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Journals / Türk Biyokimya Dergisi / 2013 / Cilt: 38 - Sayı: 4

Age-related changes in the activity and expression of manganese superoxide dismutase, and mitochondrial oxidant generation in female and male rats

Dişi ve erkek sıçanların mangan superoksit dismutaz aktivitesi ve ekspreyonu ile mitokondriyal oksidan üretiminde yaşla ilişkili değişiklikler

Evin ADEMOGLU
Koray OZCAN
Figen GURDOL
Sevda Tanrikulu KUCUK
Pages
445–450
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

The gender- and age-related differences were observed in MnSOD expression (p

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The aim of this study was to examine the age- and gender-related differences in peroxide production, manganese superoxide dismutase (MnSOD) activity and expression in liver mitochondria of Wistar rats at 12 and 24 months of age.
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Key Findings & Data:Results: Mitochondrial peroxides are increased significantly in both genders as aging proceeded, and females exhibited more profound increment than the males.
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Conclusion & Impact:The gender- and age-related differences were observed in MnSOD expression (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The aim of this study was to examine the age- and gender-related differences in peroxide production, manganese superoxide dismutase (MnSOD) activity and expression in liver mitochondria of Wistar rats at 12 and 24 months of age. Methods: The chemiluminometric method for peroxide production, fluorometric method for malondialdehyde (MDA) levels, cumene hydroperoxide assay for glutathione peroxidase (GPx), the nitroblue tetrazolium assay for MnSOD activity, and Western-blotting for MnSOD expression were used. Results: Mitochondrial peroxides are increased significantly in both genders as aging proceeded, and females exhibited more profound increment than the males. Mitochondrial SOD and GPx activities remained unaltered between 12 and 24 months of age, with no difference between two genders. The gender- and age-related differences were observed in MnSOD expression (p

Özet

Amaç: Çalışmamızda, 12 ve 24 aylık Wistar cinsi sıçanların karaciğer mitokondrilerinde yaş ve cinsiyet ile ilişkili olarak peroksit üretimi, mangan süperoksit dismutaz (MnSOD) aktivitesi ve ekspresyonundaki ortaya çıkan değişikliklerin incelenmesi amaçlanmıştır. Yöntemler: Peroksit üretimi kemilümünometrik, malondialdehit (MDA) düzeyi florometrik, glutatyon peroksidaz (GPx) kümen hidroperoksit, MnSOD aktivitesi nitroblue tetrazolium yöntemleri ile MnSOD ekspresyonu ise western blotting yöntemi ile ölçülmüştür. Bulgular: Her iki cinsiyette de yaş ilerledikçe SOD ve GPx aktivitelerinde anlamlı bir değişiklik oluşmadığı, mitokondriyal peroksit üretiminin ise anlamlı şekilde arttığı ve bu artışın dişi sıçanlarda daha belirgin olduğu saptanmıştır. MnSOD ekspresyonun da yaş ve cinsiyete bağlı olarak değişiklikler gösterdiği (p

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Cite This Article

ADEMOGLU, E., OZCAN, K., GURDOL, F., KUCUK, S. T. (2022). Age-related changes in the activity and expression of manganese superoxide dismutase, and mitochondrial oxidant generation in female and male rats. Türk Biyokimya Dergisi, Cilt: 38 - Sayı: 4, 445–450. https://ajindex.com/articles/621250

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