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Journals / Türk Biyokimya Dergisi / 2013 / Cilt: 38 - Sayı: 4

Myelin basic protein profile of central nervous system in experimentally induced demyelination and remyelination

Deneysel demiyelinasyon ve remiyelinasyonda merkezi sinir sisteminin miyelin temel protein profili

Mehmet Önder KARAYİĞİT
Murat YARIM
Gül Fatma YARIM
Gülay ÇİFTCİ
Pages
451–456
Publication Date
2022-11-03
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This study will contribute to the understanding of the mechanisms of demyelination and remyelination as well as will serve a base for the future studies investigating diseases characterized by demyelination in human and animals

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The aim of the present study was to assess myelin basic protein (MBP) profiles of central nervous system in experimentally induced demyelination and remyelination.
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Key Findings & Data:Materials and Methods: Sixty 8-10 weeks old male C57Bl/6 mice were used for this study.
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Conclusion & Impact:This study will contribute to the understanding of the mechanisms of demyelination and remyelination as well as will serve a base for the future studies investigating diseases characterized by demyelination in human and animals
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The aim of the present study was to assess myelin basic protein (MBP) profiles of central nervous system in experimentally induced demyelination and remyelination. Materials and Methods: Sixty 8-10 weeks old male C57Bl/6 mice were used for this study. Twenty mice were selected as control and 40 mice as experimental group. Experimental group was fed ad libitum with a 0.3 % cuprizone diet for 6 weeks. At the end of 6 weeks, 20 mice from the experimental group and 10 mice from the control group were euthanasied and the remaining of the experimental group was fed cuprizone-free diet for more 4 weeks and then 10 mice in the control group and 20 mice in the experimental group were sacrificed. Corpus callosum sections were removed and protein extraction was performed in all samples. The MBP profiles were evaluated by sodium dodecyl sulphate - polyacrylamide gel electrophoresis and Western-blot analysis. Results and Conclusion: Decreased intensity of the MBP isoforms below the 26.6 kDa was observed in demyelinated group as compared with controls. In the remylinated group, intensity of MBP approached to values of control group. This study will contribute to the understanding of the mechanisms of demyelination and remyelination as well as will serve a base for the future studies investigating diseases characterized by demyelination in human and animals

Özet

Amaç: Bu çalışmanın amacı, deneysel demiyelinasyon ve remiyelinasyonda merkezi sinir sisteminin miyelin temel protein (MBP) profilini değerlendirmektir.Gereç ve Yöntemler: Bu çalışmada 60 adet, 8-10 haftalık erkek C57BL/6 fare kullanıldı. Farelerden yirmi adeti kontrol grubu, 40 adeti deneme grubu olarak ayrıldı. Deneme grubundaki fareler 6 hafta boyunca % 0.3 kuprizon içeren diyet ile beslendi. Bu süre sonunda, deneme grubundan 20 fare ile kontrol grubundan 10 farenin ötanazileri yapıldı. Deneme grubundaki diğer 20 fare ile kontrol grubundaki 10 fare, remiyelinasyon oluşturmak amacıya, 4 hafta boyunca kuprizon içermeyen diyet ile beslendi ve sakrifiye edildi. Farelerin korpus kallosumları çıkartıldı ve tüm örneklerde protein ekstraksiyonu yapıldı. MBP profilleri SDSPAGE ve Western-blot analizi ile değerlendirildi.Bulgular ve Tartışma: Demiyelinasyon grubunda 26.6 kDa altındaki MBP izoformlarının yoğunluğunun kontrol grubuna göre azaldığı gözlendi. Remiyelinasyon grubunda MBP yoğunluğunun kontrol grubundaki düzeye yaklaştığı belirlendi. Bu çalışma, demiyelinasyon ve remiyelinasyon mekanizmalarının anlaşılmasına katkıda bulunmasının yanı sıra, insan ve hayvanlarda demiyelinasyon ile karakterize hastalıklarla ilgili çalışmalar için temel teşkil edecektir

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Cite This Article

KARAYİĞİT, M. Ö., YARIM, M., YARIM, G. F., ÇİFTCİ, G. (2022). Myelin basic protein profile of central nervous system in experimentally induced demyelination and remyelination. Türk Biyokimya Dergisi, Cilt: 38 - Sayı: 4, 451–456. https://ajindex.com/articles/621234

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