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Journals / Türk Biyokimya Dergisi / 2014 / Cilt: 39 - Sayı: 1

The Effects of high fat diets with and without N-Acetylcysteine supplementation on the lipin-1 levels of serum and various tissues in rats

Sıçanlarda N-asetilsistein takviyeli ve takviyesiz yüksek yağlı diyetin serum ve çeşitli doku lipin-1 seviyelerine etkisi

Sinan CANPOLAT
Hanife KARA
Birgül Vanizor KURAL
Buket AKCAN
Cemil KAHRAMAN
Ahmet ALVER
Diler Us ALTAY
Pages
19–24
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The lipin-1 levels in serum and tissues were determined by using Rat ELISA kit.Results: In comparison to serum lipin-1 levels in HFD group were significantly lower, but heart lipin-1 levels were significantly higher (P

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: Obesity is a public health problem, but the struggle against obesity continues to increase every day.
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Key Findings & Data:Lipin-1 is an adipokine that modulates lipid metabolism and glucose homeostasis.
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Conclusion & Impact:The lipin-1 levels in serum and tissues were determined by using Rat ELISA kit.Results: In comparison to serum lipin-1 levels in HFD group were significantly lower, but heart lipin-1 levels were significantly higher (P
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Obesity is a public health problem, but the struggle against obesity continues to increase every day. Lipin-1 is an adipokine that modulates lipid metabolism and glucose homeostasis. The aims of the current study were to compare the levels of lipin-1 in serum and tissues (liver, lung, heart, pancreas and kidney) in rat fed with low fat diet (LFD), high fat diet (HFD) and N-acetylcysteine (NAC) supplemented HFD, and to determine the relationships of the lipin-1 levels in serum and tissues with the levels of serum glucose and lipid parameters. Methods: 18 male Sprague-Dawley rats were divided into three groups: LFD group (n=6), HFD group (n=6) and the NAC-supplemented HFD (HFD+NAC) group (n=6). The feeding period of the diets was 85 days. The lipin-1 levels in serum and tissues were determined by using Rat ELISA kit.Results: In comparison to serum lipin-1 levels in HFD group were significantly lower, but heart lipin-1 levels were significantly higher (P

Özet

Amaç: Obezite, bir halk sağlığı sorunudur. Ancak, obeziteyle mücadele her geçen gün artmaya devam etmektedir. Lipin-1, lipid metabolizması ve glukoz homeostazını düzenleyen bir adipokindir. Bu çalışmanın amacı; düşük yağlı diyet (LFD), yüksek yağlı diyet (HFD) ve N-asetilsistein (NAC)-takviyeli HFD ile beslenen sıçanların serum ve dokularında (pankreas, karaciğer, akciğer, kalp ve böbrek) lipin-1 seviyelerini karşılaştırmak ve bu lipin-1 seviyelerinin serum glukoz ve lipid parametreleri ile olan ilişkisini belirlemekti.Yöntemler: 18 erkek Sprague-Dawley sıçan üç gruba ayrıldı: LFD grubu (n = 6), HFD grubu (n = 6) ve NAC-takviyeli HFD (HFD + NAC) grubu (n = 6). Diyetler ile beslenme süresi 85 gündü. Serum ve dokularda lipin-1 seviyeleri ELISA kiti kullanılarak belirlendi.Bulgular: HFD grubunda serum lipin-1 seviyeleri LFD grubundan anlamlı düşük, kalp lipin-1 seviyeleri ise anlamlı yüksek bulundu (P

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Cite This Article

CANPOLAT, S., KARA, H., KURAL, B. V., AKCAN, B., KAHRAMAN, C., ALVER, A., ALTAY, D. U. (2022). The Effects of high fat diets with and without N-Acetylcysteine supplementation on the lipin-1 levels of serum and various tissues in rats. Türk Biyokimya Dergisi, Cilt: 39 - Sayı: 1, 19–24. https://ajindex.com/articles/621189

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