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Journals / Türk Biyokimya Dergisi / 2014 / Cilt: 39 - Sayı: 1

Antioxidant and antimicrobial activity of East Black Sea İY Region honeys

Doğu Karadeniz Bölgesi ballarının antioksidan ve antimikrobiyal aktiviteleri

Ömer ERTÜRK
Melek Çol AYVAZ
Sevgi KOLAYLI
Hüseyin ŞAHİN
Pages
99–106
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The samples showed moderate antimicrobial activity against many microorganisms.Conclusion: All the analyzed East Black Sea Region honey samples demonstrated antioxidant and antimicrobial activity level can be considered effective.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Honey possesses antioxidant and antimicrobial activities.
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Key Findings & Data:Many chronic diseases are associated with increased oxidative stress caused by an imbalance between free-radical production and the antioxidant level.
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Conclusion & Impact:The samples showed moderate antimicrobial activity against many microorganisms.Conclusion: All the analyzed East Black Sea Region honey samples demonstrated antioxidant and antimicrobial activity level can be considered effective.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Honey possesses antioxidant and antimicrobial activities. Many chronic diseases are associated with increased oxidative stress caused by an imbalance between free-radical production and the antioxidant level. For that purpose, the total phenolic contents, antioxidant potentials and antimicrobial activities of nine honey samples obtained from East Black Sea Region was investigated. Methods: The average phenolic contents for honey samples obtained from East Black Sea Region was determined according to Folin-Ciocalteu method. For evaluation of the antioxidant activity three different methods were used, the ferric reducing antioxidant power (FRAP) assay, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity assay and cupric reducing antioxidant capacity (CUPRAC) assay. The antimicrobial activity was studied by the disc diffusion method, using ten bacteria and three yeasts.Results: The average phenolic content for these samples was determined as 0.224 mg gallic acid equivalents per g honey. According to FRAP assay, antioxidative activity of honeys was between 0.973 and 9.053 ?mol FeSO4.7H2O/g. But the average CUPRAC activity was found as 7.815 mol Trolox/g honey. IC50 values were found as between 29.388 and 458.450 mg/mL at the end of DPPH radical scavenging activity assay. The samples showed moderate antimicrobial activity against many microorganisms.Conclusion: All the analyzed East Black Sea Region honey samples demonstrated antioxidant and antimicrobial activity level can be considered effective.

Özet

Amaç: Bal antioksidan ve antimikrobiyal aktiviteye sahiptir. Çoğu kronik hastalık serbest radikal üretimi ile antioksidan seviye arasındaki dengesizliğin sebep olduğu artan oksidatif stres ile ilişkilidir. Bu amaçla Doğu Karadeniz Bölgesinden elde edilen 9 bal örneğinin toplam fenolik içerikleri, antioksidan potansiyelleri ve antimikrobiyal aktiviteleri incelenmiştir. Yöntemler: Doğu Karadeniz Bölgesinden elde edilen bal örneklerinin ortalama fenolik içrikleri Folin-Ciocalteu yöntemine göre belirlendi. Antioksidan aktivitenin değerlendirilmesi için üç farklı yöntem kullanılmıştır: demir indirgeyici antioksidan güç (FRAP) testi, 1,1-difenil-2-pikrilhidrazil (DPPH) radikali süpürme aktivitesi testi ve bakır indirgeyici antioksidan kapasite (CUPRAC) testi. Antimikrobiyal aktivite disk difüzyon tekniğine göre on bakteri ve üç maya kullanılarak incelendi. Bulgular: Bu örnekler için ortalama fenolik içerik 1 g bal örneği için gallik asit eşdeğeri olarak 0.224 mg olarak belirlenmiştir. FRAP testine göre 1 g bal örnekleri için antioksidan aktivite 0.973 ve 9.053 ?mol FeSO4.7H2O değerleri arasındadır. Ortalama CUPRAC aktivitesi troloks eşdeğeri olarak 1 g bal örneği için 7.815 mol olarak bulunmuştur. DPPH radikal süpürme aktivitesi testinin sonucunda IC50 değerleri 29.388 ve 458.450 mg/mL arasında bulunmuştur. Örnekler çoğu mikroorganizmaya karşı orta derecede antimikrobiyal aktivite göstermiştir. Sonuç: Doğu Karadeniz Bölgesinden elde edilen çalışılan tüm bal örnekleri önemli sayılabilecek derecede antioksidan ve antimikrobiyal aktiviteye sahiptir.

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Cite This Article

ERTÜRK, Ö., AYVAZ, M. Ç., KOLAYLI, S., ŞAHİN, H. (2022). Antioxidant and antimicrobial activity of East Black Sea İY Region honeys. Türk Biyokimya Dergisi, Cilt: 39 - Sayı: 1, 99–106. https://ajindex.com/articles/621183

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