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Journals / Türk Biyokimya Dergisi / 2015 / Cilt: 40 - Sayı: 6

Serebral iskemi sonrası beyin dokusunda epidermal büyüme faktörü, tümör nekrozis faktör alfa ve tiyoredoksin

Epidermal growth factor, tumor necrosis factor alpha, and thioredoxin in cerebral tissue following cerebral ischemia

Nedret KILIÇ
Hatice Ferhan KÖMÜRCÜ
Serdar KAHRAMAN
Melike EROL DEMİRBİLEK
Pages
518–523
Publication Date
2022-11-05
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

Whereas TRX levels at the 48th hour in right hemisphere were higher than those in left hemisphere.Conclusion: The results suggested that cerebral ischemia following MCAo might affect EGF, TNF-α and TRX levels in both ischemic and contralateral hemispheres in cerebral tissue; and this effect may change by the time passed after MCAO.

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Objective & Hypothesis:objective: in this study, the changes in the levels of the epidermal growth factor (EGF), tumor necrosis factor alpha (TNF-α), and thioredoxine (TRX) in the cerebral tissue of rats following the cerebral ischemia model were investigated.Methods: The left middle cerebral artery occlusion (MCAo) was done in 27 out of 48 rats recruited into the study for obtaining focal cerebral ischemia model (MCAo group).
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Key Findings & Data:EGF, TNF-α, and TRX levels were studied in the operated left and contralateral hemispheres at the 16th, 48th, and 96th hours, and on the 30th day after operation in both sham and MCAo groups.Results: EGF levels at 96th hour in MCAo group were higher than those of sham group in both right and left hemispheres; and also the levels of EGF at 48th hour in MCAo group were lower significantly than those of sham group in just left hemisphere. in MCAo group, TNF-α levels on the 30th day in right hemisphere were higher than those in left hemisphere.
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Conclusion & Impact:Whereas TRX levels at the 48th hour in right hemisphere were higher than those in left hemisphere.Conclusion: The results suggested that cerebral ischemia following MCAo might affect EGF, TNF-α and TRX levels in both ischemic and contralateral hemispheres in cerebral tissue; and this effect may change by the time passed after MCAO.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

objective: in this study, the changes in the levels of the epidermal growth factor (EGF), tumor necrosis factor alpha (TNF-α), and thioredoxine (TRX) in the cerebral tissue of rats following the cerebral ischemia model were investigated.Methods: The left middle cerebral artery occlusion (MCAo) was done in 27 out of 48 rats recruited into the study for obtaining focal cerebral ischemia model (MCAo group). Twenty-one rats underwent a sham operation; the middle cerebral artery was exposed but not occluded (Sham group). EGF, TNF-α, and TRX levels were studied in the operated left and contralateral hemispheres at the 16th, 48th, and 96th hours, and on the 30th day after operation in both sham and MCAo groups.Results: EGF levels at 96th hour in MCAo group were higher than those of sham group in both right and left hemispheres; and also the levels of EGF at 48th hour in MCAo group were lower significantly than those of sham group in just left hemisphere. in MCAo group, TNF-α levels on the 30th day in right hemisphere were higher than those in left hemisphere. Whereas TRX levels at the 48th hour in right hemisphere were higher than those in left hemisphere.Conclusion: The results suggested that cerebral ischemia following MCAo might affect EGF, TNF-α and TRX levels in both ischemic and contralateral hemispheres in cerebral tissue; and this effect may change by the time passed after MCAO.

Özet

Amaç: Bu çalışmada, sıçanlarda orta serebral arter oklüzyonu yapılarak serebral iskemi modeli oluşturduktan sonra epidermal büyüme faktörü (EGF), tümör nekrozis faktör alfa (TNF-?) ve tiyoredoksin (TRX) nin beyin dokusundaki düzeylerinin değişimlerini araştırıldı.Metod: Çalışmaya dahil edilen 54 sıçan içinden 27'sinde fokal serebral iskemi modeli oluşturmak için sol hemisferde orta serebral arter oklüzyonu (oSAo) yapıldı (oSAo grubu). Taklit operasyon yapılan 21 sıçanda orta serebral arter ortaya çıkarıldı, fakat oklüde edilmedi (Taklit grubu). oSAo ve taklit gruplarında, operasyon yapılan sol hemisferde ve karşı hemisferde EGF, TNF-? ve TRX düzeyleri operasyondan sonraki 16, 48, 96. saatlerde ve 30. günde çalışıldı.Bulgular: oSAo grubunda EGF düzeyleri 96. saatte hem sağ, hem de sol hemisferde taklit grubuna gore daha yüksek bulundu; ayrıca oSAo grubunda TNF-? düzeyi 48. saatte sadece sol hemisferde anlamlı olarak shamden daha düşük bulundu. oSAo grubunda TNF-? düzeyi 30. günde sağ hemisferde sol hemisphere gore anlamlı olarak artmış bulundu. Sol hemisferde TRX düzeyi ise sadece 48. saatte anlamlı olarak sağ hemisferden yüksek bulundu.Sonuç: Bu bulgular oSAo dan sonra oluşturulan serebral iskeminin, hem iskemik hem de kontralateral hemisferde serebral dokuda EGF, TNF-? ve TRX düzeylerine etkili olabileceğini ve oSAo dan sonra geçen zamanla bu etkinin değişebileceğini düşündürmektedir

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Cite This Article

KILIÇ, N., KÖMÜRCÜ, H. F., KAHRAMAN, S., DEMİRBİLEK, M. E. (2022). Serebral iskemi sonrası beyin dokusunda epidermal büyüme faktörü, tümör nekrozis faktör alfa ve tiyoredoksin. Türk Biyokimya Dergisi, Cilt: 40 - Sayı: 6, 518–523. https://ajindex.com/articles/621158

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