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Journals / Türk Biyokimya Dergisi / 2016 / Cilt: 41 - Sayı: 1

Antinükleer antikor pozitifliği ve tiyol/disülfid düzeylerinin karşılaştırılması

The comparison of antinuclear antibody positivity and thiol/disulfide levels

Salim NEŞELİOĞLU
Özcan EREL
Ayşe AYNALİ
Buket ARIDOĞAN CİCİOĞLU
Pages
8–11
Publication Date
2022-11-05
DOI
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⚡ TL;DR(Too Long; Didn't Read)

The native thiol, total thiol levels and native thiol/disulfide, total thiol/disulfide, native thiol/total thiol ratio was found statistically significant difference among the different age groups (p

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: There is also a growing body of evidence showing that an abnormal thiol disulphide homeostasis state is involved in the pathogenesis of certain diseases.
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Key Findings & Data:The indirect immunofluorescence antinuclear antibody test (HEp 20-10, EUROIMMUN, Germany) was used.
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Conclusion & Impact:The native thiol, total thiol levels and native thiol/disulfide, total thiol/disulfide, native thiol/total thiol ratio was found statistically significant difference among the different age groups (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: There is also a growing body of evidence showing that an abnormal thiol disulphide homeostasis state is involved in the pathogenesis of certain diseases. In the present study, it was aimed to investigate the relationship among ANA positivity and dynamic thiol/disulphide homeostasis in serum samplesMethods: Serum samples were collected from ANA-positive and ANA-negative individuals. The indirect immunofluorescence antinuclear antibody test (HEp 20-10, EUROIMMUN, Germany) was used. The serum thiol/disulfide levels were measured with the fully automated new method.Results: No statistically significant difference was detected between thiol/disülfide levels in individuals who were negative and those who were positive for ANA (p>0.05). Besides, among the ANA patterns was not found statistically significant difference for thiol/disulfide levels (p>0.05). However, serum native thiol, total thiol and disülfide levels were decreased with aging. The native thiol, total thiol levels and native thiol/disulfide, total thiol/disulfide, native thiol/total thiol ratio was found statistically significant difference among the different age groups (p

Özet

Amaç: Bazı hastalıkların patogenezinde, tiyol/disülfid seviyelerinde gözlenen anormalliklerin rolünün olduğu düşünülmektedir. Bu çalışma, ANA pozitif serum örneklerinde gözlenen tiyol/disülfid seviyelerinin prognoz ile ilgili bir ipucu sağlayıp sağlayamayacağı hakkında fikir elde etmek için planlanmıştır.Metod: Bu çalışma için ANA test sonucu pozitif ve negatif olan kişilerin serum örnekleri toplanmıştır. ANA testi için İndirekt immünfloresans antinükleer antikor testi (HEp 20-10, EUROIMMUN, Almanya) kullanılmıştır. Serum tiyol/disülfid seviyeleri ise tam otomatik yeni bir yöntem ile ölçülmüştür.Bulgular: ANA-pozitif ve ANA-negatif serum örneklerinde çalışılan tiyol/disülfid düzeyleri arasında istatistiksel olarak anlamlı fark gözlenmemiştir (p>0.05). Ayrıca, ANA paternleri arasında da tiyol/disülfid düzeyleri arasında istatistiksel olarak anlamlı fark gözlenmemiştir (p>0.05). Ancak, serum nativ tiyol, total tiyol ve disülfid seviyelerinin yaşla birlikte azaldığı gözlenmiştir.Sonuç: Çalışmamızda, tiyol/disülfid düzeyleri, ANA-pozitif bireylerde normal veya anormal biyokimyasal süreçler hakkında bize ayırt edici bir ipucu sağlamamıştır. Ancak, elde etmiş olduğumuz veriler ışığında tiyol/disülfid düzeylerinin yaşa bağlı hastalıkların patogenezinde önemli bir faktör olabileceği ve bunun yapılacak yeni ve kapsamlı çalışmalar ile ayınlatılabileceği sonucuna varılmıştır

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Cite This Article

NEŞELİOĞLU, S., EREL, Ö., AYNALİ, A., CİCİOĞLU, B. A. (2022). Antinükleer antikor pozitifliği ve tiyol/disülfid düzeylerinin karşılaştırılması. Türk Biyokimya Dergisi, Cilt: 41 - Sayı: 1, 8–11. https://ajindex.com/articles/621083

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References (11)

  1. Toma V, Fabian N, Crivii Ms. Hydrocortisone action on the thiol groups of the thymus. Endocrinologie 1986; 24(4):253-5.
  2. Cremers CM, Jakob U. Oxidant sensing by reversible disulfide bond formation. J Biol Chem 2013; 288(37):26489-96.
  3. Erel O, Neselioglu s. A novel and automated assay for thiol/ disulphide homeostasis. Clin Biochem 2014; 47(18):326-32.
  4. O'sullivan M, McLean-Tooke A, Loh R. Antinuclear antibody test. Australian family physician 2013; 42:718. Meroni PL, schur PH. ANA screening: an old test with new recommendations. Ann Rheum Dis 2010; 69(8):1420-2. solomon DH, Kavanaugh AJ, schur PH; American College of Rheumatology Ad Hoc Committee on Immunologic Testing Guidelines. Evidence-based guidelines for the use of immunologic tests: antinuclear antibody testing. Arthritis Rheum 2002; 47(4):434-44.
  5. Dinser R, Braun A, Jendro MC, Engel A. Increased titres of anti-nuclear antibodies do not predict the development of associated disease in the absence of initial suggestive signs and symptoms. scand J Rehabil Med 2007; 36:448-51.
  6. Marin GG, Cardiel MH, Cornejo H, Viveros ME. Prevalence of antinuclear antibodies in 3 groups of healthy individuals: blood donors, hospital personnel, and relatives of patients with autoimmune diseases. J Clin Rheumatol 2009; 15(7):325-9.
  7. Dean RT, Fu s, stocker R, Davies MJ. Biochemistry and pathology of radical-mediated protein oxidation. Biochem J 1997; 324 ( Pt 1):1-18.
  8. Headlam HA, Davies MJ. Beta-scission of side-chain alkoxyl radicals on peptides and proteins results in the loss of side-chains as aldehydes and ketones. Free Radic Biol Med 2002; 32(11):1171-84.
  9. Filippin LI, Vercelino R, Marroni NP, Xavier RM. Redox influence on the inflammatory response in rheumatoid arthritis. Rev Bras Reumatol 2008; 48:17-24.
  10. Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 2006; 160(1):1-40. Pérez YG, Pérez LC, Netto Rde C, Lima Ds, Lima Es. Malond- ialdehyde and sulfhydryl groups as biomarkers of oxidative stress in patients with systemic lupus erythematosus. Rev Bras Reumatol 2012; 52(4):658-60. Morgan PE, sturgess AD, Davies MJ. Increased levels of serum protein oxidation and correlation with disease activity in systemic lupus erythematosus. Arthritis Rheum 2005; 52(7):2069-79.
  11. Go YM, Jones DP. Intracellular proatherogenic events and cell adhesion modulated by extracellular thiol/disulfide redox state. Circulation 2005; 111(22):2973-80. Giustar