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Journals / Türk Biyokimya Dergisi / 2016 / Cilt: 41 - Sayı: 1

GPX1 geni Pro198Leu ve OGG1 geni ser326Cys varyantlarının koroner arter hastalığındaki rolleri

The roles of the GPX1 Pro198Leu and OGG1Ser326Cys variants in coronary artery disease

Burcu BAYOĞLU
Müjgan CENGİZ
Esin KURT
Hüseyin Altuğ ÇAKMAK
Hüsniye YÜKSEL
Pages
44–50
Publication Date
2022-11-05
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Real-time polymerase chain reaction (RT-PCR) was used to genotype GPX1 Pro198Leu and OGG1 Ser326Cys polymorphisms.Results: Systolic and diastolic blood pressure, pulse, glucose, total- and LDL-cholesterol, triglycerides and waist circumference values were statistically significant between CAD patients and controls (p

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Coronary artery disease (CAD) is caused by plaque formation on inner walls of coronary arteries.
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Key Findings & Data:Glutathione peroxidase-1 (GPX1), prevents oxidative damage by detoxifying hydrogen and lipid peroxides.
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Conclusion & Impact:Real-time polymerase chain reaction (RT-PCR) was used to genotype GPX1 Pro198Leu and OGG1 Ser326Cys polymorphisms.Results: Systolic and diastolic blood pressure, pulse, glucose, total- and LDL-cholesterol, triglycerides and waist circumference values were statistically significant between CAD patients and controls (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Coronary artery disease (CAD) is caused by plaque formation on inner walls of coronary arteries. Glutathione peroxidase-1 (GPX1), prevents oxidative damage by detoxifying hydrogen and lipid peroxides. GPX1 Pro198Leu polymorphism results in oxidant/antioxidant imbalance. 8-oxoguanine DNA glycosylase-1 (OGG1) is the key enzyme in DNA repair pathway. OGG1 Ser326Cys polymorphism leads to 8-oxoG accumulation and may play a role in cardiovascular diseases. Thus, our study aims to investigate GPX1 Pro198Leu and OGG1 Ser326Cys polymorphisms in CAD patients and the possible relationship of genotypes with serum lipids and CAD severity.Methods: A total of 169 CAD patients and 173 angiographically documented healthy controls were included in our study. The lesion severity in CAD patients was determined by Gensini scoring system. The genomic DNA was isolated from peripheral blood. Real-time polymerase chain reaction (RT-PCR) was used to genotype GPX1 Pro198Leu and OGG1 Ser326Cys polymorphisms.Results: Systolic and diastolic blood pressure, pulse, glucose, total- and LDL-cholesterol, triglycerides and waist circumference values were statistically significant between CAD patients and controls (p

Özet

Amaç: Koroner arter hastalığı (KAH), koroner arterlerin iç duvarlarında plak oluşması sonucu daralması/tıkanması ile oluşmaktadır. Glutatyon peroksidaz-1 (GPX1), oksidatif hasarı, hidrojen ve lipid peroksitleri detoksifiye ederek önlemektedir. GPX1 Pro198Leu polimorfizmi oksidan/antioksidan dengesinin bozulmasına neden olabilmektedir. 8-oksoguanin DNA glikozilaz-1 (OGG1) DNA tamirinde görevli anahtar enzimdir. OGG1 Ser326Cys polimorfizmi 8-oxoG birikimine neden olmakta ve çeşitli kardiyovasküler hastalıklarda rol oynayabilmektedir. Bu bilgiler ışığında çalışmamızda, KAH tanısı alan hastalarda GPX1 Pro198Leu ve OGG1 Ser326Cys polimorfizmleri, genotip ve allel sıklıklarının belirlenmesi, genotiplerin serum lipidleri ve KAH şiddeti ile olan ilişkilerinin araştırılması amaçlanmıştır.Metod: Çalışmamıza 169 KAH hastası ve koroner anjiyografi ile koroner arterlerinde darlık saptanmayan 173 sağlıklı kontrol dahil edilmiştir. KAH'ın derecesi ve lezyon şiddeti Gensini skorlama sistemi ile belirlendi. Tüm hasta ve kontrollerin periferik kanlarından genomik DNA izolasyonunu takiben GPX1 Pro198Leu ve OGG1 Ser326Cys polimorfizmlerinin genotip sıklıkları gerçek-zamanlı polimeraz zincir reaksiyonu (RT-PCR) ile belirlendi.Bulgular: Çalışmamızda, KAH grubu ve sağlıklı kontroller demografik ve klinik özellikleri açısından karşılaştırıldığında, sistolik ve diyastolik kan basıncı, nabız, açlık kan şekeri, total-kolesterol, LDL-kolesterol, trigliserid ve bel çevresi değerleri iki grup arasında anlamlı bulundu (p

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Cite This Article

BAYOĞLU, B., CENGİZ, M., KURT, E., ÇAKMAK, H. A., YÜKSEL, H. (2022). GPX1 geni Pro198Leu ve OGG1 geni ser326Cys varyantlarının koroner arter hastalığındaki rolleri. Türk Biyokimya Dergisi, Cilt: 41 - Sayı: 1, 44–50. https://ajindex.com/articles/621041

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