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Journals / Türk Biyokimya Dergisi / 2016 / Cilt: 41 - Sayı: 2

The effects of crocin (active contstituent of saffron) treatment on brain antioxidant enzyme mRNA levels in diabetic rats

Diyabetik ratlarda safranın aktif içeriği olan krosin tedavisinin beyindeki antioksidan enzimlerin mRNA seviyeleri üzerine etkisi

Eyüp ALTINÖZ
Yılmaz ÇİĞREMİŞ
Cemal EKİCİ
Berna ÖZYAZGAN
Pages
112–117
Publication Date
2022-11-05
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Crocin given to diabetic rats significantly decreased mRNA expression of antioxidant enzymes, SOD, CAT and GPX when compared to DM group.Conclusion: The present study demonstrates that crocin can modulate mRNA expression of antioxidant enzymes, SOD, CAT and GPX and oxidative stress in the brain of the STZ induced diabetic rats

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The aim of the present study is to evaluate the effect of crocin on mRNA expression of antioxidant enzymes, SOD, CAT and GPX in the brain of the STZ induced diabetic rats.Methods: Thirty animals randomized in three groups containing ten animals in each group as follows; control (non-diabetic rats), DM (STZ-induced untreated diabetic rats), DM+crocin (STZ-induced diabetic rats treated with crocin,).
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Key Findings & Data:Crocin was given at a dose of 20 mg/kg bw/day by gavage for 21 days.Results: STZ injection caused a significant increase in mRNA expression of antioxidant enzymes, SOD, CAT and GPX when compared to control group.
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Conclusion & Impact:Crocin given to diabetic rats significantly decreased mRNA expression of antioxidant enzymes, SOD, CAT and GPX when compared to DM group.Conclusion: The present study demonstrates that crocin can modulate mRNA expression of antioxidant enzymes, SOD, CAT and GPX and oxidative stress in the brain of the STZ induced diabetic rats
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The aim of the present study is to evaluate the effect of crocin on mRNA expression of antioxidant enzymes, SOD, CAT and GPX in the brain of the STZ induced diabetic rats.Methods: Thirty animals randomized in three groups containing ten animals in each group as follows; control (non-diabetic rats), DM (STZ-induced untreated diabetic rats), DM+crocin (STZ-induced diabetic rats treated with crocin,). Crocin was given at a dose of 20 mg/kg bw/day by gavage for 21 days.Results: STZ injection caused a significant increase in mRNA expression of antioxidant enzymes, SOD, CAT and GPX when compared to control group. Crocin given to diabetic rats significantly decreased mRNA expression of antioxidant enzymes, SOD, CAT and GPX when compared to DM group.Conclusion: The present study demonstrates that crocin can modulate mRNA expression of antioxidant enzymes, SOD, CAT and GPX and oxidative stress in the brain of the STZ induced diabetic rats

Özet

Amaç: Bu çalışmanın amacı, STZ ile oluşturulan diabetik ratların beyin dokularındaki SOD, CAT ve GPX antioksidant enzimlerin mRNA gen ekspresyonları üzerine krosinin etkilerini ortaya koymaktır.Metod: Random olarak belirlenen 30 rat her grupta 10 adet olacak şekilde şu şekilde belirlenmiştir; Kontrol (Diyabet olmayan grup), DM (STZ ile diyabet yapılan grup), DM+Krosin (STZ ile diyabet yapılan ve krosin ile tedavi edilen grup). Krosin 20 mg/kg/gün dozunda 21 gün süreyle gavajla uygulanmıştır.Bulgular: STZ enjeksiyonu kontrol grubuyla karşılaştırıldığında antioksidant enzimler olan SOD, CAT ve GPX'in mRNA ekpresyonlarında dikkat çekici bir artışa sebep olmuştur. Diyabetik ratlara krosin verildiğinde SOD, CAT ve GPX'in mRNA ekpresyonlarında dikkat çekici bir düşüşe sebep olmuştur.Sonuç: Çalışmamız STZ ile oluşturulan diyabetik ratların beyin dokularında krosin'in SOD, CAT ve GPX enzimlerinin mRNA ekpresyonlarını ve oksidatif stresi düzenlediğini ortaya koymuştur

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Cite This Article

ALTINÖZ, E., ÇİĞREMİŞ, Y., EKİCİ, C., ÖZYAZGAN, B. (2022). The effects of crocin (active contstituent of saffron) treatment on brain antioxidant enzyme mRNA levels in diabetic rats. Türk Biyokimya Dergisi, Cilt: 41 - Sayı: 2, 112–117. https://ajindex.com/articles/621029

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