SavedAdd your journal

Journals / Türk Biyokimya Dergisi / 2016 / Cilt: 41 - Sayı: 6

Maclura pomifera'dan (Rafin.) Schneider elde edilen ekstre ve bazı flavonoit türevlerinin antioksidan aktivitesi ve uçucu yağ bileşimi

Antioxidant potential of some natural and semi-synthetic flavonoid derivatives and the extracts from Maclura pomifera (Rafin.) Schneider (osage orange) and its essential oil composition

Fatma SENOL SEZER
Betül DEMİRCİ
Ilkay ORHAN ERDOGAN
Margita DVORSKA
Karel SMEJKAL
Milan ZEMLICKA
Pages
403–411
Publication Date
2022-11-05
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Phytol was the major com-pound in both the leaf oils, while the fruit essential oil con-tained ?-caryophyllene as the main component (69.3%).Discussion and Conclusion: The current study covers par-ticularly antioxidant capacity of iso-osajin, iso-pomiferin, and macluraxanthone by the aforementioned methods and, among them; pomiferin seems to be a natural pos-sible antioxidant agent.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: The antioxidant potential of various extracts were obtained from the leaf and fruit of Maclura pomifera (Rafin.) Schneider (Moraceae) along with its major isoflavonoids; osajin and pomiferin, their semi-synthetic derivatives; iso-osajin and iso-pomiferin and macluraxanthone, which were assayed.Methods: The extracts and compounds were subjected to six experimental models including 2,2´-diphenyl-1-picrylhydrazyl (DPPH), N,N-dimethyl-p-phenylendiamine (DMPD+), and nitric oxide (NO) radical scavenging activity, metal-chelating capacity, ferric- (FRAP) and phosphomolibdenum-reducing antioxidant power (PRAP) assays by using ELISA methods Results: The fruit aqueous extract exerted higher scaveng-ing activity against DMPD and NO radicals, while the fruit ethyl acetate extract was the most active against DPPH radical (68.61 ± 2.53%).
📊
Key Findings & Data:Among the tested compounds, the highest DPPH (91.74 ± 0.26%) and DMPD (30.63 ± 1.31%) radical scavenging effect was observed with macluraxan-thone, while pomiferin and iso-pomiferin exhibited better activity than osajin and iso-osajin in all assays except the metal-chelation capacity assay.
💡
Conclusion & Impact:Phytol was the major com-pound in both the leaf oils, while the fruit essential oil con-tained ?-caryophyllene as the main component (69.3%).Discussion and Conclusion: The current study covers par-ticularly antioxidant capacity of iso-osajin, iso-pomiferin, and macluraxanthone by the aforementioned methods and, among them; pomiferin seems to be a natural pos-sible antioxidant agent.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The antioxidant potential of various extracts were obtained from the leaf and fruit of Maclura pomifera (Rafin.) Schneider (Moraceae) along with its major isoflavonoids; osajin and pomiferin, their semi-synthetic derivatives; iso-osajin and iso-pomiferin and macluraxanthone, which were assayed.Methods: The extracts and compounds were subjected to six experimental models including 2,2´-diphenyl-1-picrylhydrazyl (DPPH), N,N-dimethyl-p-phenylendiamine (DMPD+), and nitric oxide (NO) radical scavenging activity, metal-chelating capacity, ferric- (FRAP) and phosphomolibdenum-reducing antioxidant power (PRAP) assays by using ELISA methods Results: The fruit aqueous extract exerted higher scaveng-ing activity against DMPD and NO radicals, while the fruit ethyl acetate extract was the most active against DPPH radical (68.61 ± 2.53%). Among the tested compounds, the highest DPPH (91.74 ± 0.26%) and DMPD (30.63 ± 1.31%) radical scavenging effect was observed with macluraxan-thone, while pomiferin and iso-pomiferin exhibited better activity than osajin and iso-osajin in all assays except the metal-chelation capacity assay. Phytol was the major com-pound in both the leaf oils, while the fruit essential oil con-tained ?-caryophyllene as the main component (69.3%).Discussion and Conclusion: The current study covers par-ticularly antioxidant capacity of iso-osajin, iso-pomiferin, and macluraxanthone by the aforementioned methods and, among them; pomiferin seems to be a natural pos-sible antioxidant agent.

Özet

Amaç: Mevcut çalışmada, Maclura pomifera'nın (Rafin.) Schneider (Moraceae) yaprak ve meyvalarından elde edilen çeşitli ekstreler ile başlıca flavonoitleri olan osajin ve pomiferin, bunların yarı-sentetik türevleri olan izo-osajin ve izo-pomiferin ile makluraksanton'un antioksidan potansiyeli incelenmiştir.Metot: Ekstreler ve bileşiklere, 2,2´-difenil-1-pikrilhidrazil (DPPH), N,N-dimetil-p-fenilendiamin (DMPD+) ve nitrik oksit (NO) radikal süpürücü aktivite, metal-şelasyon kapasitesi, demir- (FRAP) ve fosfomolibdenyumindirgeme antioksidan gücü (PRAP) dahil altı metot ELISA mikrotiter yöntemi ile uygulanmıştır.Bulgular: Meyva etil asetat ekstresi DPPH radikaline karşı en aktif ekstre iken (%68.61 ± 2.53), meyvanın sulu ekstresi DMPD ve NO radikallerine karşı daha yüksek süpürücü etki göstermiştir. Test edilen bileşikler içinde, metal-şelasyon haricindeki tüm testlerde, pomiferin ve izo-pomiferin, osajin ve izo-osajin'den daha iyi aktivite gösterirken, en yüksek DPPH (%91.74 ± 0.26) ve DMPD (%30.63 ± 1.31 %) radikal süpürücü etki makluraksanton'da görülmüştür. Erkek ve dişi ağaçların yaprakları ile meyvanın uçucu yağı GC-MS ile analiz edilmiştir. Meyva uçucu yağı ?-karyofillen'i (%69.3) ana bileşen olarak taşırken, her iki yaprakta da fitol ana bileşendir.Tartışma ve Sonuç: Bu çalışma, bahsigeçen yöntemler kullanılarak özellikle izo-osajin, izo-pomiferin ve makluraksanton'un antioksidan kapasitesini kapsamaktadır ve pomiferin doğal muhtemel bir antioksidan ajan olarak görünmektedir

🔖

Cite This Article

SEZER, F. S., DEMİRCİ, B., ERDOGAN, I. O., DVORSKA, M., SMEJKAL, K., ZEMLICKA, M. (2022). Maclura pomifera'dan (Rafin.) Schneider elde edilen ekstre ve bazı flavonoit türevlerinin antioksidan aktivitesi ve uçucu yağ bileşimi. Türk Biyokimya Dergisi, Cilt: 41 - Sayı: 6, 403–411. https://ajindex.com/articles/621025

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (12)

  1. Toker G, Erdogan I. Research on the isoflavones of Maclura pomif- era (Rafin.) Schneider fruits. J Fac Pharm Gazi Univ 1998;15:29-34.
  2. Monache GD, Scurria R, Vitali A, Botta A, Monacelli B, Pasqua G, et al. Two isoflavones and a flavone from the fruits of Maclura pomifera. Phytochemistry 1994;37:893-8.
  3. Teixeira DM, Costa CT. Novel methods to extract flavanones and xanthones from the root bark of Maclura pomifera. J Chrom A 2005;1062:175-81.
  4. Darji K, Miglis C, Wardlow A, Abourashed EA. HPLC determination of isoflavone levels in osage orange from the midwest and southern United States. J Agric Food Chem 2013;61:6806-11.
  5. Peterson C, Zhu J, Coats JR. Identification of components of osage orange fruit (Maclura pomifera) and their repellency to German cockroaches. J Ess Oil Res 2002;14:233-6.
  6. Pearce V, Nawaz Z, Xiao W, Wiedenfeld D, Boyle N, Smith D. 4-Ethoxymethylphenol: a novel phytoestrogen that acts as an agonist for human estrogen receptors. J Steroid Biochem Mol Biol 2003;84:431-9. Bartosíkova T, Jurica J, Florian T, Klusákova J, Frydrych M. Protec- tive effects of osajin in ischemia-reperfusion of laboratory rat kidney. Pharmazie 2006;61:552-5.
  7. Kupeli E, Orhan I, Toker G, Yesilada E. Anti-inflammatory and antinociceptive potential of Maclura pomifera (Rafin.) Schnei der fruit extracts and its major isoflavonoids, scandenone and auriculasin. J Ethnopharmacol 2006;107:169-74.
  8. Orhan I, Senol FS, Kartal M, Dvorská M, Zemlicka M, Smejkal K, et al. Cholinesterase inhibitory effects of the extracts and compounds of Maclura pomifera (Rafin.) Schneider. Food Chem Toxicol 2009;47:1747-51.
  9. Zhao D, Yao C, Chen X, Xia H, Zhang L, Liu H, et al. The fruits of Maclura pomifera extracts inhibits glioma stem-like cell growth and invasion. Neurochem Res 2013;38:2105-13. Moon HI. Effect of osajin and pomiferin on antidiabetic effects from normal and streptozotocin-induced diabetic rats. Nat Prod Commun 2014;9:1723-4.
  10. Khan MT, Orhan I, Senol FS, Kartal M, Sener B, Dvorská M, et al. Cholinesterase inhibitory activities of some flavonoid derivatives and chosen xanthone and their molecular docking studies. Chem-Biol Interact 2009;181:383-9.
  11. Wolfrom ML, Mahan J. Osage orange pigments. IX. Improved separation; establishment of the isopropylidene group. J Org Chem 1942;64:308-10. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;181:1199-200.
  12. Schlesier K, Harvat M, Bohm V, Bitsch R. Assessment of antioxi- dant activity by using different in vitro