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Journals / Türk Biyokimya Dergisi / 2018 / Cilt: 43 - Sayı: 4

Oxidative stress and response of antioxidant system in Nostoc muscorum exposed to different forms of Zinc

Farklı Çinko formlarına maruz bırakılan Nostoc muscorum‘da oksidatif stres ve antioksidan sistemin tepkisi

Sadaf JAHAN
Arbab HUSAIN
Alvina FAROOQUİ
Sadaf MAHFOOZ
Adeeba SHAMİM
Pages
352–361
Publication Date
2022-11-05
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

The non-enzymatic antioxidants such asproline and total phenols also showed enhanced accumulationsup to 16 and 64 μM of ZnCl2 and N-ZnO treatment,respectively.Conclusion: Our results for the very first time depictedthe significant accumulation of stress biomarkersin N. muscorum in response to deleterious effects ofoxidative stress caused by N-ZnO, ZnCl2, and M-ZnO.Present results revealed varying levels of toxicity ofvarious forms of zinc, which decreased in an order ofZnCl2 > N-ZnO > M-ZnO.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Present study aims to evaluate oxidative stressmediatedtoxicity mechanisms of nano-ZnO (N-ZnO) comparedto ZnCl2 and bulk-ZnO (M-ZnO), using cyanobacteriumas a toxicity assessment tool.Methods: Nostoc muscorum was used as a test organismand the experiments were performed in parallels aftertreated with 2–128 μM of N-ZnO, M-ZnO, and ZnCl2.Results: Low concentrations of ZnCl2 (2–8 μM) and N-ZnO(16–32 μM) led to a significant increase in the carotenoids,which could be ascribed to its role as an antioxidant,whereas higher concentrations were inhibitory to the photosyntheticpigments.
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Key Findings & Data:Enhanced activities of superoxidedismutase (SOD), peroxidase (POD) and accelerated lipidperoxidation depicted significant accumulation of superoxideand peroxide radicals in N. muscorum exposed toN-ZnO and ZnCl2.
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Conclusion & Impact:The non-enzymatic antioxidants such asproline and total phenols also showed enhanced accumulationsup to 16 and 64 μM of ZnCl2 and N-ZnO treatment,respectively.Conclusion: Our results for the very first time depictedthe significant accumulation of stress biomarkersin N. muscorum in response to deleterious effects ofoxidative stress caused by N-ZnO, ZnCl2, and M-ZnO.Present results revealed varying levels of toxicity ofvarious forms of zinc, which decreased in an order ofZnCl2 > N-ZnO > M-ZnO.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Present study aims to evaluate oxidative stressmediatedtoxicity mechanisms of nano-ZnO (N-ZnO) comparedto ZnCl2 and bulk-ZnO (M-ZnO), using cyanobacteriumas a toxicity assessment tool.Methods: Nostoc muscorum was used as a test organismand the experiments were performed in parallels aftertreated with 2–128 μM of N-ZnO, M-ZnO, and ZnCl2.Results: Low concentrations of ZnCl2 (2–8 μM) and N-ZnO(16–32 μM) led to a significant increase in the carotenoids,which could be ascribed to its role as an antioxidant,whereas higher concentrations were inhibitory to the photosyntheticpigments. Enhanced activities of superoxidedismutase (SOD), peroxidase (POD) and accelerated lipidperoxidation depicted significant accumulation of superoxideand peroxide radicals in N. muscorum exposed toN-ZnO and ZnCl2. The non-enzymatic antioxidants such asproline and total phenols also showed enhanced accumulationsup to 16 and 64 μM of ZnCl2 and N-ZnO treatment,respectively.Conclusion: Our results for the very first time depictedthe significant accumulation of stress biomarkersin N. muscorum in response to deleterious effects ofoxidative stress caused by N-ZnO, ZnCl2, and M-ZnO.Present results revealed varying levels of toxicity ofvarious forms of zinc, which decreased in an order ofZnCl2 > N-ZnO > M-ZnO.

Özet

Amaç: Bu çalışmada, toksisite değerlendirme aracı olarak siyanobakteri kullanarak ZnCl2 ve bulk-ZnO (M-ZnO) ile karşılaştırıldığında, nano-ZnO‘in (N-ZnO) oksidatif stres aracılı toksisite mekanizmasını değerlendirmeyi amaçladık. Yöntemler: Bir test organizması olarak Nostoc muscorum kullanımıştır, deneyler parallel olarak 2–128 μM N-ZnO, M-ZnO ve ZnCl2 ile muamele edildikten sonra gerçekleştirilmiştir. Bulgular: Düşük konsantrasyonda ZnCl2 (2–8 μM) ve N-ZnO (16–32 μM) antioksidan rolünü destekleyen bir şekilde, karotenoidlerde belirgin bir artış göstermiştir, bunun yanısıra daha yüksek konsantrasyonlar fotosentetik pigmentleri inhibe etmiştir. Süperoksit dismutaz (SOD), peroksidaz (POD) aktivitelerindeki artış ve hızlandırılmış lipit peroksidasyonu, N-ZnO ve ZnCl2‘ye maruz bırakılan N. muscorum‘da süperoksit ve peroksit radikallerinin önemli bir birikimine işaret etmiştir. 16 μM ZnCl2 ve 64 μM N-ZnO uygulamaları sonucunda Prolin ve toplam fenoller gibi enzimatik olmayan antioksidanlarda da yüksek birikimler gösterilmiştir. Sonuç: Bu çalışmanın sonuçları ilk kez, N-ZnO, ZnCl2 ve M-ZnO‘nun neden olduğu zararlı etkilere yanıt olarak N. muscorum‘da oksidatif stres biyobelirteçlerinin önemli birikiminin olduğunu göstermiştir. Mevcut sonuçlar ZnCl2 > N-ZnO > M-ZnO sıralamasında, çeşitli çinko formlarının, düşen toksisitesinin seviyelerini ortaya koymuştur.

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Cite This Article

JAHAN, S., HUSAIN, A., FAROOQUİ, A., MAHFOOZ, S., SHAMİM, A. (2022). Oxidative stress and response of antioxidant system in Nostoc muscorum exposed to different forms of Zinc. Türk Biyokimya Dergisi, Cilt: 43 - Sayı: 4, 352–361. https://ajindex.com/articles/620991

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