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Journals / Türk Biyokimya Dergisi / 2018 / Cilt: 43 - Sayı: 4

Betaine treatment decreased serum glucose and lipid levels, hepatic and renal oxidative stress in streptozotocin-induced diabetic rats

Betain tedavisi streptozotosin ile diabet oluşturulan sıçanlarda serum glikoz ve lipit düzeylerini, karaciğer ve böbreklerde oksidatif stresi azalttı

Abdurrahman Fatih AYDIN
Mehmet SAR
Semra Doğru ABBASOĞLU
Müjdat UYSAL
Buse UĞURALP
Betül EVRAN
Pages
343–351
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

BETdiminished hepatic and renal prooxidant status.Conclusion: Our results indicate that BET may be effectivein decreasing STZ-induced high levels of blood HbA1c, andserum glucose and lipid levels and prooxidant status inliver and kidney tissues.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The present study was aimed to investigate theeffects of betaine (BET) on streptozotocin (STZ)-induceddiabetes mellitus (DM) in rats.
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Key Findings & Data:Additionally, the efficiencyof BET was compared with metformin (MET), a standardoral antidiabetic drug.Methods: STZ (55 mg/kg body weight; i.p.) was injectedto male Wistar rats.
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Conclusion & Impact:BETdiminished hepatic and renal prooxidant status.Conclusion: Our results indicate that BET may be effectivein decreasing STZ-induced high levels of blood HbA1c, andserum glucose and lipid levels and prooxidant status inliver and kidney tissues.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The present study was aimed to investigate theeffects of betaine (BET) on streptozotocin (STZ)-induceddiabetes mellitus (DM) in rats. Additionally, the efficiencyof BET was compared with metformin (MET), a standardoral antidiabetic drug.Methods: STZ (55 mg/kg body weight; i.p.) was injectedto male Wistar rats. Rats with DM were treated withBET (1 g/kg body weight/day;) or MET (500 mg/kg bodyweight/day;) for 4 weeks. Blood glycated hemoglobin(HbA1c), serum glucose, lipids, hepatic and renal functiontests and urinary protein levels were examined. Reactiveoxygen species (ROS) formation, malondialdehyde (MDA),glutathione (GSH) levels, and ferric reducing antioxidantpower (FRAP) were also determined in liver and kidney.Results: Glucose, HbA1c, and serum lipids increased andliver and kidney function tests were impaired in diabeticrats. Hepatic and renal ROS formation and MDA levelswere elevated, hepatic, but not renal GSH and FRAPlevels were decreased. BET decreased blood HbA1c, serumglucose and lipid levels and urine protein levels. BETdiminished hepatic and renal prooxidant status.Conclusion: Our results indicate that BET may be effectivein decreasing STZ-induced high levels of blood HbA1c, andserum glucose and lipid levels and prooxidant status inliver and kidney tissues.

Özet

Amaç: Bu çalışmada betainin (BET) streptozotosin (STZ) ile diabet oluşturulan sıçanlarda etkisini araştırmak amaçlandı. Ayrıca BET’in etkisi standart oral antidiyabetik ilaç olan metforminle (MET) karşılaştırıldı. Metod: STZ (55 mg/kg vücut ağırlığı;i.p.) erkek Wistar sıçanlara uygulandı. 4 hafta süreyle diyabetik sıçanlara BET (1/ kg vücut ağırlığı/gün; diyetle) veya MET (500 mg/kg vücut ağırlığı/gün; içme suyunda) uygulaması yapıldı. Kanda glike hemoglobin (HbA1c), serumda glikoz, lipid ve karaciğer ve böbrek fonksiyon testleri ve idrarda protein düzeyleri ölçüldü. Ayrıca, karaciğer ve böbrek dokusunda reaktif oksijen türleri oluşumu, malondialdehit (MDA), glutatyon (GSH) düzeyleri ve antioksidan aktivite (FRAP) tayin edildi.Bulgular: Diyabetik sıçanlarda glikoz, HbA1c ve serum lipit düzeyleri arttı, karaciğer ve böbrek fonksiyon testleri bozuldu. Karaciğer ve böbrekte ROS oluşumu ve MDA düzeyleri arttı, karaciğerde GSH ve FRAP düzeyleri azaldı. BET uygulaması kan HbA1c, serum glikoz ve lipit düzeylerini ve idrar protein düzeylerini azalttı. BET ayrıca karaciğer ve böbrekte prooksidan durumu baskıladı. Sonuç: Sonuçlarımız BET’in STZ uygulamasıyla artmış olan kan HbA1c, serum glikoz ve lipit düzeyleri ile karaciğer ve böbrek dokularında prooksidan durumu azaltmada etkili olduğunu gösterdi.

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Cite This Article

AYDIN, A. F., SAR, M., ABBASOĞLU, S. D., UYSAL, M., UĞURALP, B., EVRAN, B. (2022). Betaine treatment decreased serum glucose and lipid levels, hepatic and renal oxidative stress in streptozotocin-induced diabetic rats. Türk Biyokimya Dergisi, Cilt: 43 - Sayı: 4, 343–351. https://ajindex.com/articles/620984

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References (15)

  1. Kassab A, Piwowar A. Cell oxidant stress delivery and cell dysfunction onset in type 2 diabetes. Biochimie 2012;94:1837–48
  2. De M Bandeira S, Da Fonseca LJ, Da S Guedes G, Rabelo LA, Goulart MO, Vasconcelos SM. Oxidative stress as an underlying contributor in the development of chronic complications in diabetes mellitus. Int J Mol Sci 2013;14:3265–84
  3. Forbes JM, Cooper ME. Mechanisms of diabetic complications. Physiol Rev 2013;93:137–88
  4. Karaağaç N, Salman F, Doğru-Abbasoğlu S, Uysal M. Changes in prooxidant-antioxidant balance in tissues of rats following longterm hyperglycemic status. Endocr Res 2011;36:124–33
  5. Ong KW,Hsu A, Song L, Huang D, Tan BK. Polyphenols-rich Vernonia amygdalina shows anti-diabetic effects in streptozotocininduced diabetic rats. J Ethnopharmacol 2011;133:598–607
  6. Alhaider AA, Korashy HM, Sayed-Ahmed MM, Mobark M, Kfoury H, Mansour MA. Metformin attenuates streptozotocin-induced diabetic nephropathy in rats through modulation of oxidative stress genes expression. Chem Biol Interact 2011;192:233–42
  7. Hao HH, Shao ZM, Tang DQ, Lu Q, Chen X, Yin XX, et al. Preventive effects of rutin on the development of experimental diabetic nephropathy in rats. Life Sci 2012;91:959–67
  8. İbrahim DS, El-Maksoud MA. Effect of strawberry (Fragaria x ananassa) leaves extract on diabetic nephropathy in rats. Int J Exp Path 2015;96:87–93
  9. Dey A, Lakshmanan J. The role of antioxidants and other agents in alleviating hyperglycemia mediated oxidative stress and injury in liver. Food Funct 2013;4:1148–84
  10. Ueland PM, Holm PI, Hustad S. Betaine: a key modulator of onecarbon metabolism and homocysteine status. Clin Chem Lab Med 2005;43:1069–75
  11. Day CR, Kempson SA. Betaine chemistry, roles, and potential use in liver disease. Biochim Biophys Acta 2016;1860:1098–106
  12. Lv S, Fan R, Du Y, Hou M, Tang Z, Ling W, et al. Betaine supplementation attenuates atherosclerotic lesion in apolipoprotein E-deficient mice. Eur J Nutr 2009;48:205–12
  13. Balkan J, Öztezcan S, Küçük M, Çevikbaş U, Koçak-Toker N, Uysal M. The effect of betaine treatment on triglyceride levels and oxidative stress in the liver of ethanol-treated guinea pigs. Exp Toxicol Pathol 2004;55:505–9
  14. Kwon DY, Jung YS, Kim SJ, Park HK, Park JH, Kim YC. Impaired sulfur-amino acid metabolism and oxidative stress in nonalcoholic fatty liver are alleviated by betaine supplementation in rats. J Nutr 2009;139:63–8
  15. Balkan J, Parıldar FH, Doğru-Abbasoğlu S, Aykaç-Toker G, U