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Journals / Türk Biyokimya Dergisi / 2018 / Cilt: 43 - Sayı: 4

Evaluation of resveratrol organogels prepared by micro-irradiation: fibroblast proliferation through in vitro wound healing

Mikrodalga yöntemi ile hazırlanan resveratrol içeren organojellerin fibroblast proliferasyonuna etkisinin in vitro yara iyileşme modeliyle değerlendirilmesi

Ayşe NALBANTSOY
Ceren KEÇECİLER
Çiğdem ÇELEN
Evren Homan GÖKÇE
Evren ALĞIN YAPAR
Pages
385–392
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Thevalues obtained with RSV-PEG-200 and RSV-PEG-600 were36.6% and 48.7%, respectively.Conclusion: This study showed that RSV organogels producedby micro-irradiation method could be a model foractive molecules that have low water solubility for differentapplications as pharmaceuticals, and cosmeceuticals.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Resveratrol (RSV) has therapeutic potentialwith several biological activity.
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Key Findings & Data:Three different types of polyethyleneglycol (200, 400 and 600) were used to prepare Carbopol940 based organogels by micro-irradiation method.
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Conclusion & Impact:Thevalues obtained with RSV-PEG-200 and RSV-PEG-600 were36.6% and 48.7%, respectively.Conclusion: This study showed that RSV organogels producedby micro-irradiation method could be a model foractive molecules that have low water solubility for differentapplications as pharmaceuticals, and cosmeceuticals.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Resveratrol (RSV) has therapeutic potentialwith several biological activity. The aim of this study wasto develop organogel formulations of RSV and to investigatethe proliferation and migration via in vitro woundmodel of primary normal human skin fibroblasts (NHDF).Methods: The optimum RSV concentration was determinedby MTT assay. Three different types of polyethyleneglycol (200, 400 and 600) were used to prepare Carbopol940 based organogels by micro-irradiation method. Differentialscanning microscopy (DSC) and rheological analyseswere conducted. Proliferation activity and migration offibroblast cells were determined with the Giemsa staining.Results: The percentage of migration rate obtained withRSV-PEG-400 organogel was the highest, as 59.7%. Thevalues obtained with RSV-PEG-200 and RSV-PEG-600 were36.6% and 48.7%, respectively.Conclusion: This study showed that RSV organogels producedby micro-irradiation method could be a model foractive molecules that have low water solubility for differentapplications as pharmaceuticals, and cosmeceuticals.

Özet

Amaç: Resveratrol (RSV) pek çok biyolojik aktivite ve terapötik potansiyele sahip bir moleküldür. Çalışmanın amacı RSV içeren organojel formülasyonları geliştirmek ve NHDF (Normal Human Dermal Fibroblast) hücreleri ile oluşturulan in vitro yara modeli kullanılarak proliferasyon ve migrasyon üzerindeki etkinliğini belirlemektir. Yöntemler: Organojel içeriğindeki en uygun RSV konsantrasyonu MTT testi ile belirlenmiştir. Üç farklı tip polietilen glikol (200, 400 ve 600) ve Carbopol 940 kullanılarak mikrodalga uygulaması ile organojeller elde edilmiştir. Oluşturulan organojel formülasyonlarının DSC ve reolojik analizleri gerçekleştirilmiştir. İn vitro yara modeli üzerinde Giemsa boyama ile RSV içeren organojellerin fibroblast hücrelerinin proliferasyon aktivitesi ve migrasyonu üzerindeki etkinliği belirlenmiştir. Sonuçlar: En yüksek migrasyon oranı PEG-400 ile oluşturulan RSV içeren organojellerde %59.7 oranında belirlenmiştir. RSV-PEG-200 ve RSV-PEG-600 ise sırasıyla 36.6% ve 48.7% olarak belirlenmiştir. Tartışma: Bu çalışma mikrodalga yöntemi ile geliştirilen RSV içeren organojel formülasyonları çözünürlüğü düşük aktif moleküllerin farmasötik ve kozmetik gibi farklı uygulama alanlarında kullanımı için model olarak kullanılabilir.

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Cite This Article

NALBANTSOY, A., KEÇECİLER, C., ÇELEN, Ç., GÖKÇE, E. H., YAPAR, E. A. (2022). Evaluation of resveratrol organogels prepared by micro-irradiation: fibroblast proliferation through in vitro wound healing. Türk Biyokimya Dergisi, Cilt: 43 - Sayı: 4, 385–392. https://ajindex.com/articles/620978

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