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Journals / Türk Biyokimya Dergisi / 2018 / Cilt: 43 - Sayı: 4

Effects of epigallocatechin-3-gallate (EGCG) on a scleroderma model of fibrosis

Epigallokateşin-3-gallat’ın (EGCG) Skleroderma Fibrozis Modeli Üzerine Etkileri

Osman Yılmaz
Zahide ÇAVDAR
Sulen SARIOĞLU
Gul GUNER
Duygu HARMANCI
Ayse KOCAK
Merih BİRLİK
Pages
464–473
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

MMP-1, MMP-8 protein levels wereincreased and p-SMAD 2/3 protein level was decreased.TGF-β mRNA expression was decreased in the EGCG + BLMgroup compared with the BLM group.Conclusion: These results suggest an antifibrotic role forEGCG.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The aim of the present study was to evaluatethe potential protective effects of epigallocatechin-3-gallate(EGCG) on fibrosis in bleomycin induced sclerodermamodel.Materials and methods: Thirty-two healthy female Balb-cmice with the average body weight of 22 ± 5 g were used inthis study.
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Key Findings & Data:The mice were randomly divided into four groupsas control (n = 8), Bleomycin (n = 8), Bleomycin + EGCG(n = 8) and EGCG (n = 8).
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Conclusion & Impact:MMP-1, MMP-8 protein levels wereincreased and p-SMAD 2/3 protein level was decreased.TGF-β mRNA expression was decreased in the EGCG + BLMgroup compared with the BLM group.Conclusion: These results suggest an antifibrotic role forEGCG.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The aim of the present study was to evaluatethe potential protective effects of epigallocatechin-3-gallate(EGCG) on fibrosis in bleomycin induced sclerodermamodel.Materials and methods: Thirty-two healthy female Balb-cmice with the average body weight of 22 ± 5 g were used inthis study. The mice were randomly divided into four groupsas control (n = 8), Bleomycin (n = 8), Bleomycin + EGCG(n = 8) and EGCG (n = 8). Skin tissue samples were collectedto quantify matrix metalloproteinases (MMP-1,MMP-8, MMP-13), p-SMAD 2/3 and SMAD 2/3 in proteinhomogenates by western blotting. TGF-β1 expression wasdetermined by real-time PCR. Immunohistopathologicaland histopathological examinations of skin tissues werealso done.Results: When measured with Masson Trichrome, EGCGtreatment was found to decrease fibrosis in connectivetissue compared to the BLM injected control. EGCG wasdecreased dermal fibrosis. Bleomycin + EGCG groupshowed a significant reduction in fibrosis at the dermalsurface area using hematoxylin measurements comparedwith the BLM group. MMP-1, MMP-8 protein levels wereincreased and p-SMAD 2/3 protein level was decreased.TGF-β mRNA expression was decreased in the EGCG + BLMgroup compared with the BLM group.Conclusion: These results suggest an antifibrotic role forEGCG.

Özet

Amaç: Bu çalışmanın amacı, bleomisinle (BLM) oluşturulmuş skleroderma modelinde epigallokateşin-3-gallat’ın (EGCG) fibrozis üzerine olası koruyucu etkilerini değerlendirmektir. Gereç ve Yöntem: Bu çalışmada ortalama vücut ağırlığı 22 ± 5 g olan 32 adet sağlıklı dişi Balb-c fare kullanıldı. Fareler rastgele olarak; kontrol (n = 8), Bleomisin (n = 8), Bleomisin + EGCG (n = 8) ve EGCG (n = 8) olmak üzere dört gruba ayrıldı. p-SMAD 2/3, SMAD 2/3 ve matriks metalloproteinaz (MMP-1, MMP-8, MMP-13) proteinlerinin western blotting tayini için dermal doku örnekleri toplandı. TGF-β1 mRNA ekspresyonu qPCR ile belirlendi. Deri dokularında immünohistopatolojik ve histopatolojik incelemeler yapıldı. Sonuçlar: Masson Trikrom boyama sonuçlarına göre, EGCG uygulanan grupta fibrozisin azaldığı gözlendi. Hematoksilen- eozin boyama sonuçlarına göre, Bleomisin + EGCG grubu ile BLM grubu karşılaştırıldığında EGCG uygulanan grupta fibrozis anlamlı ölçüde azaldı. EGCG uygulanan grupta MMP-1, MMP-8 protein düzeylerinin arttığı ve p-SMAD 2/3 protein düzeyinin azaldığı belirlendi. BLM ve BLM + EGCG grupları karşılaştırıldığında TGF-β mRNA ekspresyonunun EGCG uygulanan grupta azaldığı gözlendi. Sonuç: Sklerodermada, EGCG antifibrotik bir rol oynamaktadır.

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Cite This Article

Yılmaz, O., ÇAVDAR, Z., SARIOĞLU, S., GUNER, G., HARMANCI, D., KOCAK, A., BİRLİK, M. (2022). Effects of epigallocatechin-3-gallate (EGCG) on a scleroderma model of fibrosis. Türk Biyokimya Dergisi, Cilt: 43 - Sayı: 4, 464–473. https://ajindex.com/articles/620972

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