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Journals / Türk Biyokimya Dergisi / 2018 / Cilt: 43 - Sayı: 3

Comparative study of stirred and fluidized tank reactor for hydroxyl-kojic acid derivatives synthesis and their biological activities

Hidroksil-kojik asit türevleri sentezi ve biyolojik aktiviteleri için karıştırımalı ve akışkanlaştırılmış tank reaktörlerinin karşılaştırmalı çalışması

Muhajir HAMID
Syahida AHMAD
Arbakariya B. ARIFF
Ahmad Firdaus B. LAJIS
Pages
205–219
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

HUFA-KA could be used aspotential lipophilic antioxidant while HSFA-KA has thepotential to be used to treat melanoma skin disorder.

📌 3 Key Takeaways

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Objective & Hypothesis:Background: Study on the synthesis of kojic acid derivatives(KADs) in solvent-free system using scalable reactorsand their biological activities is still lacking.Methods: In this study, two types of KADs, were synthesizedusing saturated-fatty acid [lauric acid (LA)] and unsaturatedfattyacid [oleic acid (OA)] in stirred tank reactor (STR) andfluidized tank reactor (FTR).
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Key Findings & Data:The yield and biological activitiesof the synthesized KADs were evaluated and compared.Results: The highest yield of KADs (42.95%) was obtainedin the synthesis using OA, with molar ratio of 1:1, enzymeloading of 5% (w/v), temperature of 70°C, using immobilizedlipase N435 in STR.
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Conclusion & Impact:HUFA-KA could be used aspotential lipophilic antioxidant while HSFA-KA has thepotential to be used to treat melanoma skin disorder.
🔬Design:Research Article
📍Scope:2000 Verileri
⚙️Variables:Domain Parameters

Abstract

Background: Study on the synthesis of kojic acid derivatives(KADs) in solvent-free system using scalable reactorsand their biological activities is still lacking.Methods: In this study, two types of KADs, were synthesizedusing saturated-fatty acid [lauric acid (LA)] and unsaturatedfattyacid [oleic acid (OA)] in stirred tank reactor (STR) andfluidized tank reactor (FTR). The yield and biological activitiesof the synthesized KADs were evaluated and compared.Results: The highest yield of KADs (42.95%) was obtainedin the synthesis using OA, with molar ratio of 1:1, enzymeloading of 5% (w/v), temperature of 70°C, using immobilizedlipase N435 in STR. However, FTR may provide biocatalystprotection and reusability with reduced loss ofKADs yield up to three cycles. In antioxidant assay, thehydroxyl-unsaturated-fatty acid of kojic acid (HUFA-KA)showed better activity as compared to hydroxyl-saturated-fatty acid of kojic acid (HSFA-KA) at concentrationsranging from 125 to 2000 μg/mL. In contrast, HSFA-KAshowed better cytotoxicity effect against G361 melanomacell as compared to HUFA-KA.Conclusion: The yield of KADs obtained in STR was higherthan that obtained in FTR. HUFA-KA could be used aspotential lipophilic antioxidant while HSFA-KA has thepotential to be used to treat melanoma skin disorder.

Özet

Giriş: Kojik asit türevlerinin (KAD‘ler) ölçeklenebilir reaktörler ile, çözücü içermeyen sistemlerde sentezi ve biyolojik faaliyetleri konusunda literatürde eksiklikler bulunmaktadır. Metod: Bu çalışmada, karıştırılmış tank reaktöründe (STR) ve akışkanlaştırılmış tank reaktöründe (FTR) doymuş yağ asiti (laurik asit) ve doymamış yağ asidi (oleik asit) kullanılarak iki tip KAD sentezi yapılmıştır. Sentezlenen KAD‘lerin verimi ve biyolojik aktiviteleri değerlendirilmiş ve karşılaştırılmıştır. Bulgular: KAD‘ler için en yüksek verim (%42.95), STR‘da sabitleştirilmiş lipaz N435 varlığında, 1:1 mol oranında oleik asit kullanılarak, enzim yükü %5 (ağırlık / hacim), 70°C sıcaklıktaki sentez koşulları altında elde edilmiştir. Buna karşın, FTR, biyokatalizörü koruyarak, KAD‘lerin kaybını azaltabilir ve üç döngüye kadar yeniden kullanılabilirliğini sağlayarak verimi arttırabilir. Anti oksidan tahlilinde, kojik asitin (HUFA-KA) hidroksil-doymamış yağ asiti 125 ila 2000 μg/mL arasında değişen konsantrasyonlarda kojik asitin (HSFA-KA) hidroksil doymuş yağ asidi ile karşılaştırıldığında daha iyi etkinlik göstermiştir. Aksine, HSFA-KA, HFA-KA‘ya kıyasla G361 melanoma hücrelerinde daha fazla sitotoksisite etkisi sergilemişitir. Sonuç: STR‘de elde edilen KAD‘lerin verimi FTR‘de elde edilen verimden daha yüksekti. HSFA-KA, melanoma cilt bozukluğunu tedavi etme potansiyeline sahipken, HUFA-KA potansiyel lipofilik antioksidan olarak kullanılabilir.

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Cite This Article

HAMID, M., AHMAD, S., ARIFF, A. B., LAJIS, A. F. B. (2022). Comparative study of stirred and fluidized tank reactor for hydroxyl-kojic acid derivatives synthesis and their biological activities. Türk Biyokimya Dergisi, Cilt: 43 - Sayı: 3, 205–219. https://ajindex.com/articles/620955

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