Journals / Türk Biyokimya Dergisi / 2018 / Cilt: 43 - Sayı: 4

Wendlandia exserta: a pertinent source of antioxidant and antimicrobial agent

Wendlandia exserta: Antioksidan ve antimikrobial maddeler için uygun bir kaynak

Pages
456–463
Publication Date
2022-11-05
DOI
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The fractionsalso displayed good antimicrobial activity against variousmicroorganisms.Conclusions: It was concluded that polar fractions ofW. exserta showed good antioxidant and antimicrobialactivity, so these might be useful in pharmacologicalpreparations.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The aim of the present investigation was toassess the antioxidant, antibacterial and antifungal activitiesof various fractions of Wendlandia exserta (RoxbukanPansara).Methods: The methanolic extract of W. exserta (RoxbukanPansara) was dissolved in water (distilled) and thenpartitioned with solvents e.g. n-hexane, CHCl3, EtOAc andn-BuOH consecutively.
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Key Findings & Data:Antimicrobial activity wasexamined by four bacteria and four fungi by the disc diffusionmethod by using a standard, as positive control.Results: The aqueous fraction displayed maximum valueof diphenyl picryl hydrazyl (DPPH) radical inhibition(85.94% ± 0.35) with an IC50 value of 415.31 ± 0.26 μg mL−1.The aqueous fraction also displayed the maximum ferricreducing antioxidant power (FRAP) value [64.5 ± 0.15 μgof trolox equivalents (TE)] as well as maximum inhibitionof lipid peroxidation (42.32% ± 0.59) and maximum valueof total phenolic contents (87.10 ± 1.10).
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Conclusion & Impact:The fractionsalso displayed good antimicrobial activity against variousmicroorganisms.Conclusions: It was concluded that polar fractions ofW. exserta showed good antioxidant and antimicrobialactivity, so these might be useful in pharmacologicalpreparations.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: The aim of the present investigation was toassess the antioxidant, antibacterial and antifungal activitiesof various fractions of Wendlandia exserta (RoxbukanPansara).Methods: The methanolic extract of W. exserta (RoxbukanPansara) was dissolved in water (distilled) and thenpartitioned with solvents e.g. n-hexane, CHCl3, EtOAc andn-BuOH consecutively. Antioxidant potential of abovementioned fractions and remaining aqueous fractionwas checked by four methods. Antimicrobial activity wasexamined by four bacteria and four fungi by the disc diffusionmethod by using a standard, as positive control.Results: The aqueous fraction displayed maximum valueof diphenyl picryl hydrazyl (DPPH) radical inhibition(85.94% ± 0.35) with an IC50 value of 415.31 ± 0.26 μg mL−1.The aqueous fraction also displayed the maximum ferricreducing antioxidant power (FRAP) value [64.5 ± 0.15 μgof trolox equivalents (TE)] as well as maximum inhibitionof lipid peroxidation (42.32% ± 0.59) and maximum valueof total phenolic contents (87.10 ± 1.10). However, the n-BuOH fraction was found to display the maximum valueof total antioxidant activity (0.529 ± 0.76). The fractionsalso displayed good antimicrobial activity against variousmicroorganisms.Conclusions: It was concluded that polar fractions ofW. exserta showed good antioxidant and antimicrobialactivity, so these might be useful in pharmacologicalpreparations.

Özet

Amaç: Bu araştırmanın amacı, Wendlandia exserta (Roxbukan Pansara) ’nın çeşitli fraksiyonlarının antioksidan, antibakteriyel ve antifungal aktivitelerini değerlendirmektir. Yöntemler: Wendlandia exserta’nın (Roxbukan Pansara) metanolik ekstraktı suda çözülmüş (damıtılmış) ve daha sonra farklı çözücülerde, ör. n-heksan, CHCl3, EtOAc ve n-BuOH sırayla ayrıştırılmıştır. Yukarıda belirtilen fraksiyonların ve kalan sulu fraksiyonun antioksidan etkisi dört yöntemle kontrol edildi. Antimikrobiyal aktivite, standart olarak pozitif kontrol kullanılarak disk difüzyon yöntemi ile dört bakteri ve dört mantar üzerinde incelendi. Bulgular: Sulu fraksiyon 415,31 ± 0,26 μg mL−1 IC50 değeri ile DPPH radikal inhibisyonunun maksimum değerini (% 85,94 ± 0,35) gösterdi. Sulu fraksiyon ayrıca maksimum lipoproteinasyon inhibisyonu (% 42,32 ± 0,59) ve toplam fenolik içeriğin maksimum değeri (87,10 ± 1,10) yanı sıra maksimum FRAP değeri (64,5 ± 0,15 μg troloks eşdeğerleri) göstermiştir. Bununla birlikte, n-BuOH fraksiyonunun toplam antioksidan etkinliğinin en yüksek (0,529 ± 0.76) olduğu bulunmuştur. Fraksiyonlar ayrıca çeşitli mikroorganizmalara karşı iyi antimikrobiyal etki göstermiştir. Sonuçlar: Wendlandia exserta’nın polar fraksiyonlarının iyi antioksidan ve antimikrobiyal aktivite gösterdiği sonucuna varılmış, bu nedenle bu fraksiyonların farmakolojik preparatlarda yararlı olabileceği sonucuna varılmıştır.

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Cite This Article

SHAHID, M., SHAHZADI, T., RIAZ, T., AJAIB, M., MAZHAR, F., ABBASI, M. A. (2022). Wendlandia exserta: a pertinent source of antioxidant and antimicrobial agent. Türk Biyokimya Dergisi, Cilt: 43 - Sayı: 4, 456–463. https://ajindex.com/articles/620952

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References (15)

  1. Prashant KR, Dolly J, Singh KR, Gupta KR, Watal G. Glycemic properties of Trichosanthes dioica leaves. Pharm Biol 2008;46:894–9
  2. Kusumaa IW, Murdiyantoa, Arunga ET, Syafrizal, Kim Y. Antimicrobial and antioxidant properties of medicinal plants used by the Bentian tribe from Indonesia. Food Sci Human Wellness 2014;3:191–6
  3. Morales G, Paredes A, Sierra P, Loyola LA. Antioxidant activity of 50% aqueous-ethanol extract from Acantholippia deserticola. Biol Res 2008;41:151–5
  4. Devasagayam TP, Tilak JC, Boloor KK, Ketaki SS, Saroj SG, Lele RD. Review: free radical and antioxidants in human health current status and future prospects. J Assoc Physicians India 2004;53:794–804
  5. Mccall MR, Frei B. Can antioxidant vitamins materially reduce oxidative damage in humans? Free radical Bio Med 1999;26:1034–53
  6. Zima TS, Fialova L, Mestek O, Janebová M, Crkovská J, Malbohan I, et al. Oxidative stress, metabolism of ethanol and alcoholrelated diseases. J Bio Sci 2001;8:59–70
  7. Atoui AK, Mansouri A, Boskou G, Panagiotis K. Tea and herbal infusions: their antioxidant activity and phenolic profile. Food Chem 2005;89:27–36
  8. Halliwell B. Gutteridge JM. Free radicals in biology and medicine, 2nd ed. Oxford: Clarendon Press, 1989
  9. Hras AR, Hadolin M, Knez Z, Bauman D. Comparison of antioxidative and synergistic effects of osemary extract with alpha-tocopherol, ascorbylpalmitate and citric acid in sunflower oil. Food Chem 2000;71:229–33
  10. Peschel W, Sanchez-Rabaneda F, Dieckmann W, Plescher A, Gartzia I. An industrial approach in the search of natural antioxidants from vegetable and fruits wastes. Food Chem 2006;97:137–50
  11. Anagnostopoulou MA, Kefalas P, Papageorgiou VP, Boskou D. Radical scavenging activity of various extracts and fractions of sweet orange peel (Citrus sinensis). Food Chem 2006; 94:19–25
  12. Hao YY, Brackett RE, Doyle MP. Efficacy of plant extracts in inhibiting Aeromonas hydrophilia and Listeria monocytogenes in refrigerated cooked poultry. Food Microbiol 1998;15:367–78
  13. Nazimuddin S, Qaiser M. Rubiaceae. In: Nasir E, Ali SI, editors. Flora of Pakistan. Karachi, Pakistan: University of Karachi, 1989:190
  14. Lee K, Shibamoto T. Antioxidant property of aroma extract isolated from dove buds [Syzygiumaromatlcum (L.) Men: Et Perry]. Food Chem 2001;74:443
  15. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determina