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Journals / Türk Biyokimya Dergisi / 2018 / Cilt: 43 - Sayı: 2

Evaluation of apoptotic caspase levels in estimation of the wound age

Yara yaşı tahmininde apoptotik kaspaz düzeylerinin değerlendirilmesi

Atiye Seda YAR SAĞLAM
Pınar UYAR GÖÇÜN
Ece KONAC
Taner AKAR
Ebru ALP
Adnan MENEVŞE
Pages
126–133
Publication Date
2022-11-04
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

We reported a statistically significantincrease in Caspase 3, 8 and 9-positive cells on days 3 and5 after immunohistochemical staining as well.Conclusion: Our results suggest that time-dependent featuresof apoptotic factors might offer a potential tool inestimating wound age.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: We aimed to investigate the potential use ofthe expression of apoptotic signaling pathway genes of ratin skin wound age estimation.Material and methods: For this purpose, we formed cuttingtool injuries using a scalpel in an experimental model.Then, we assessed Caspase 3, 8 and 9 mRNA levels by usingquantitative real-time PCR and protein levels by usingimmunohistochemistry in rat skin wounds.
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Key Findings & Data:In addition, weused TUNEL assay to detect apoptotic cells.Results: We observed that Caspase 3 mRNA level significantlyincreased (2.1 ± 0.4 folds) on day 3 (p < 0.05) andCaspase 8 mRNA level significantly increased (1.8 ± 0.2folds) on day 5 (p < 0.05).
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Conclusion & Impact:We reported a statistically significantincrease in Caspase 3, 8 and 9-positive cells on days 3 and5 after immunohistochemical staining as well.Conclusion: Our results suggest that time-dependent featuresof apoptotic factors might offer a potential tool inestimating wound age.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objectives: We aimed to investigate the potential use ofthe expression of apoptotic signaling pathway genes of ratin skin wound age estimation.Material and methods: For this purpose, we formed cuttingtool injuries using a scalpel in an experimental model.Then, we assessed Caspase 3, 8 and 9 mRNA levels by usingquantitative real-time PCR and protein levels by usingimmunohistochemistry in rat skin wounds. In addition, weused TUNEL assay to detect apoptotic cells.Results: We observed that Caspase 3 mRNA level significantlyincreased (2.1 ± 0.4 folds) on day 3 (p < 0.05) andCaspase 8 mRNA level significantly increased (1.8 ± 0.2folds) on day 5 (p < 0.05). Caspase 9 mRNA level increased(1.9 ± 0.1 folds) on day 3 and (2.5 ± 0.4 folds) on day 5(p < 0.05). The percentage values of polymorphonuclearleukocytes (PMNLs) and inflammatory mononuclear cells(IMCs) were observed after immunohistochemical stainingby Caspase 3, 8, 9 antibodies. Our immunohistochemistryresults were found to be consistent with the mRNAresults observed. We reported a statistically significantincrease in Caspase 3, 8 and 9-positive cells on days 3 and5 after immunohistochemical staining as well.Conclusion: Our results suggest that time-dependent featuresof apoptotic factors might offer a potential tool inestimating wound age.

Özet

Amaç: Sıçan derisindeki cilt yara yaşı tahmininde, apoptotik sinyal yolağı genlerinin ifadelenmesinin potansiyel kullanımını araştırmayı amaçladık. Gereç ve Yöntem: Bu amaçla, deneysel modelde bir neşter kullanarak kesici alet yaralanmaları oluşturduk. Daha sonra sıçan cilt yaralarında kantitatif gerçek zamanlı PCR ile Caspase 3, 8 ve 9 mRNA düzeylerini ve immünohistokimya yöntemi ile protein düzeylerini değerlendirdik. Buna ilaveten, apoptotik hücreleri saptamak için TUNEL yöntemini kullandık. Bulgular: Kaspaz 3 mRNA düzeyinin 3. günde (p < 0.05) belirgin olarak arttığı (2.1 ± 0.4 kat) ve Kaspaz 8 mRNA düzeyinin 5. günde (p < 0.05) belirgin olarak arttığı (1.8 ± 0.2 kat) gözlendi. Kaspaz 9 mRNA düzeyi 3. günde (1.9 ± 0.1 kat) arttı ve 5. günde (2.5 ± 0.4 kat) arttı (p < 0.05). Polimorfonükleer lökositlerin (PMNL) ve inflamatuar mononükleer hücrelerin (IMC) yüzde değerleri Kaspaz 3, 8, 9 antikorları ile immünhistokimyasal boyama sonrasında gözlendi. İmmünohistokimya sonuçlarımızın gözlenen mRNA sonuçları ile tutarlı olduğu bulundu. İmmünohistokimyasal boyamadan sonra 3. ve 5. günlerde Kaspaz 3, 8 ve 9 pozitif hücrelerde istatistiksel olarak anlamlı bir artış olduğunu belirledik. Sonuç: Bulgularımız, apoptotik faktörlerin zamana bağlı özelliklerinin yara yaşını tahmin etmede potansiyel bir araç olabileceğini düşündürmektedir.

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Cite This Article

SAĞLAM, A. S. Y., GÖÇÜN, P. U., KONAC, E., AKAR, T., ALP, E., MENEVŞE, A. (2022). Evaluation of apoptotic caspase levels in estimation of the wound age. Türk Biyokimya Dergisi, Cilt: 43 - Sayı: 2, 126–133. https://ajindex.com/articles/620921

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