SavedAdd your journal

Journals / Türk Biyokimya Dergisi / 2019 / Cilt: 44 - Sayı: 6

In vitro apoptotic effect of Zinc(II) complex with N-donor heterocyclic ligand on breast cancer cells

N-donör heterosiklik ligandlı çinko(II) kompleksinin meme kanseri hücreleri üzerinde in vitro apoptotik etkisi

Gamze GÜNEY ESKİLER
İbrahim KANİ
Pages
761–768
Publication Date
2022-11-05
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

However, furtherstudies are warranted to identify the underlying mechanisms of apoptotic cell death and to reduce the toxicity ofZn(II) complex on control cells.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Background: The synthesis of new ligand and transitionmetal complexes have drawn great attention in cancertreatment due to excellent DNA cleavage activities andhigh antitumor activity.Objective: The purpose of this study was to synthesizea new Zn(II) complex with 2,2′-bipyridine (bpy) ligand,$left[Zn{left(bpyright)}_2left(H_2Oright)right]2left(CIO_4right)$, and to investigate the potentialtherapeutic activity against breast cancer.
📊
Key Findings & Data:The cytotoxic and apoptoticeffects of Zn(II) complex on MCF7 breast cancer andHUVEC control cells were evaluated by WST-1, Annexin Vand cell cycle analysis.Results: The spectroscopic data demonstrated that Zn(II)complex was successfully synthesized.
💡
Conclusion & Impact:However, furtherstudies are warranted to identify the underlying mechanisms of apoptotic cell death and to reduce the toxicity ofZn(II) complex on control cells.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background: The synthesis of new ligand and transitionmetal complexes have drawn great attention in cancertreatment due to excellent DNA cleavage activities andhigh antitumor activity.Objective: The purpose of this study was to synthesizea new Zn(II) complex with 2,2′-bipyridine (bpy) ligand,$left[Zn{left(bpyright)}_2left(H_2Oright)right]2left(CIO_4right)$, and to investigate the potentialtherapeutic activity against breast cancer. Materials and methods: Zn(II) complex was obtainedand structurally characterized using crystallography andother spectroscopic methods. The cytotoxic and apoptoticeffects of Zn(II) complex on MCF7 breast cancer andHUVEC control cells were evaluated by WST-1, Annexin Vand cell cycle analysis.Results: The spectroscopic data demonstrated that Zn(II)complex was successfully synthesized. Furthermore,Zn(II) complex exhibited significant cytotoxic effect onMCF7 breast cancer and HUVEC cells (p < 0.01). MCF7and HUVEC cells proliferation was reduced to 11.0%and 52.6%, respectively at 10 μM for 48 h. Additionally,Zn(II) complex significantly induced apoptotic cell death(84.87%) and caused S phase arrest (36.4%) in MCF7 cellsat 10 μM (p < 0.01).Conclusions: Zn(II) complex has great therapeutic potential for the treatment of breast cancer. However, furtherstudies are warranted to identify the underlying mechanisms of apoptotic cell death and to reduce the toxicity ofZn(II) complex on control cells.

Özet

Amaç: Yeni ligand ve geçiş metali komplekslerinin sentezi, DNA kırılması aktiviteleri ve yüksek antitümör aktivitesi nedeniyle kanser tedavisinde önemli derecede dikkat çekmektedir. Bu çalışmanın amacı 2,2′-bipiridin (bpy) ligandı, $left[Zn{left(bpyright)}_2left(H_2Oright)right]2left(CIO_4right)$ ile yeni bir Zn(II) kompleksi sentezlemek ve meme kanserinde potansiyel terapötik etkisinin araştırılmasıdır. Gereç ve Yöntem: Zn(II) kompleksi elde edildikten sonra yapısal olarak kristalografi ve diğer spektroskopik yöntemler kullanılarak karakterize edilmiştir. Zn(II) kompleksinin MCF7 meme kanseri ve HUVEC kontrol hücreleri üzerindeki sitotoksik ve apoptotik etkileri, WST-1, Annexin V ve hücre döngüsü analizi ile değerlendirilmiştir. Bulgular: Spektroskopik veriler, Zn(II) kompleksinin başarıyla sentezlendiğini göstermektedir. Zn(II) kompleksi, MCF7 meme kanseri ve HUVEC hücreleri üzerinde önemli sitotoksik etki göstermiştir (p < 0.01). 48 saat boyunca 10 μM Zn(II) kompleksi uygulanan MCF7 ve HUVEC hücrelerinde canlılık oranlarının sırasıyla %11.0 ve %52.6’ya azaldığı belirlenmiştir. Ayrıca, 10 μM Zn(II) kompleksi MCF7 hücrelerinde apoptotik hücre ölümünü (%84.87) önemli ölçüde arttırmıştır ve hücrelerde S fazında tutulmaya (%36.4) neden olmuştur (p < 0.01). Sonuç: Zn(II) kompleksinin, meme kanseri tedavisinde önemli terapötik potansiyele sahip olduğu belirlenmiştir. Ancak, apoptotik hücre ölümüne neden olan mekanizmaları tanımlamak ve Zn(II) kompleksinin kontrol hücreleri üzerindeki toksisitesini azaltmak için ileri çalışmaların yapılması gereklidir.

🔖

Cite This Article

ESKİLER, G. G., KANİ, İ. (2022). In vitro apoptotic effect of Zinc(II) complex with N-donor heterocyclic ligand on breast cancer cells. Türk Biyokimya Dergisi, Cilt: 44 - Sayı: 6, 761–768. https://ajindex.com/articles/620883

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (17)

  1. Ndagi U, Mhlongo N, Soliman ME. Metal complexes in cancer therapy – an update from drug design perspective. Drug Des Devel Ther 2017;11:599
  2. Baile MB, Kolhe NS, Deotarse PP, Jain AS, Kulkarni AA. Metal ion complex-potential anticancer drug – a review. Int. J Pharm Sci Rev Res 2015;4:59–66
  3. Rafique S, Idrees M, Nasim A, Akbar H, Athar A. Transition metal complexes as potential therapeutic agents. Biotechnol Mol Biol Rev 2010;5:38–45
  4. Liguori PF, Valentini A, Palma M, Bellusci A, Bernardini S, Ghedini M, et al. Non-classical anticancer agents: synthesis and biological evaluation of zinc(II) heteroleptic complexes. Dalton Trans 2010;39:4205–12
  5. Komiyama M, Takeda N, Shigekawa H. Hydrolysis of DNA and RNA by lanthanide ions: mechanistic studies leading to new applications. Chem Commun 1999;16:1443–51
  6. Niittymäki T, Lönnberg H. Artificial ribonucleases. Org Biomol Chem 2006;4:15–25
  7. Milacic V, Chen D, Giovagnini L, Diez A, Fregona D, Dou QP. Pyrrolidine dithiocarbamate-zinc(II) and-copper(II) complexes induce apoptosis in tumor cells by inhibiting the proteasomal activity. Toxicol Appl Pharmacol 2008;231:24–33
  8. Cui LJ, Zhang B, Liu CY, Bian M. Two Zinc(II) and Nickel(II)-based complexes: anti breast cancer activity. Z Anorg Allg Chem 2017;643:1139–42
  9. Rundstadler T, Eskandari A, Norman S, Suntharalingam K. Polypyridyl Zinc(II)-indomethacin complexes with potent antibreast cancer stem cell activity. Molecules 2018;23:2253
  10. Milosavljevic V, Haddad Y, Rodrigo MA, Moulick A, Polanska H, Hynek D, et al. The Zinc-Schiff base-novicidin complex as a potential prostate cancer therapy. PLoS One 2016;11:e0163983
  11. Frezza M, Hindo S, Chen D, Davenport A, Schmitt S, Tomco D, et al. Novel metals and metal complexes as platforms for cancer therapy. Curr Pharm Des 2010;16:1813–25
  12. Fukuda H, Ebara M, Yamada H, Arimoto M, Okabe S, Obu M, et al. Trace elements and cancer. Japan Med Assoc J 2004;47:391–5
  13. Franklin RB, Costello LC. The important role of the apoptotic effects of zinc in the development of cancers. J Cell Biochem 2009;106:750–7
  14. Sheldrick GM. Smart Bruker AXS. Inc., Madison, WI, USA, 2000
  15. Sheldrick GM. SHELXS-2014, program for the crystal structure solution. Göttingen, Germany: University of Göttingen, 2014
  16. Macrae CF, Edgington PR, McCabe P, Pidcock E, Shields GP, Taylor R, et al. Mercury: visualization and analysis of crystal structures. J Appl Cryst 2006;39:453
  17. Spek AL. Tool PAMC. Multipurpose crystallogr