AI Summary & Key Takeaways
AI InsightIn our study, we aimed toinvestigate effects of α-lactalbumin and sulindac on COLO320 primary and COLO-741 metastatic human colon carcinoma cell lines via mitochondrial apoptotic pathway byindirect immunocytochemistry and TUNEL assays.Materials and methods: The effects of α-lactalbuminand sulindac were assessed by using MTT assay at 24, 48and 72 h and apoptosis markers caspase-3, caspase-9 andcytochrome-c were detected using immunocytochemistryand TUNEL methods.Results: It was appeared that α-lactalbumin and sulindacmay triggger mechanisms of apoptosis in both primaryand metastatic colon carcinoma cell lines and the primarycolon carcinoma cell line was affected more than the metastatic cells.Conclusion: It is proposed that α-lactalbumin and sulindac can be used in cancer treatments and future in vivoexperiments.
📌 3 Key Takeaways
Abstract
Background: HAMLET (human α-lactalbumin made lethalto tumor cells) and sulindac are active ingredients whichare used in the treatment of cancers. Under both in vivo andin vitro conditions, HAMLET binds to the surface of tumorcells and enters the cells. Sulindac is one of nonsteroidalanti-inflammatory drugs. It inhibits the growth of tumorcells by inducing the apoptosis. In our study, we aimed toinvestigate effects of α-lactalbumin and sulindac on COLO320 primary and COLO-741 metastatic human colon carcinoma cell lines via mitochondrial apoptotic pathway byindirect immunocytochemistry and TUNEL assays.Materials and methods: The effects of α-lactalbuminand sulindac were assessed by using MTT assay at 24, 48and 72 h and apoptosis markers caspase-3, caspase-9 andcytochrome-c were detected using immunocytochemistryand TUNEL methods.Results: It was appeared that α-lactalbumin and sulindacmay triggger mechanisms of apoptosis in both primaryand metastatic colon carcinoma cell lines and the primarycolon carcinoma cell line was affected more than the metastatic cells.Conclusion: It is proposed that α-lactalbumin and sulindac can be used in cancer treatments and future in vivoexperiments.
Özet
Amaç: HAMLET (human α-lactalbumin made lethal to tumor cells) ve sulindac, kanser tedavisinde kullanılan etken maddelerdir. HAMLET, in vivo ve in vitro koşullar altında, tümör hücrelerinin yüzeyine bağlanarak hücre içerisine girer. Sulindac, non-steroidal bir anti-inflamatuvar ilaçlardan biridir ve apoptozu tetikleyerek tümör hücrelerinin çoğalmasını inhibe eder. Çalışmamızda α-laktalbumin ve sulindac’ın COLO-320 primer ve COLO741 metastatik insan kolon karsinomu hücre dizinleri üzerindeki etkilerini, mitokondriyal apoptotik yolak aracılığı ile immünositokimya ve TUNEL yöntemleri ile araştırmayı amaçladık. Gereç ve Yöntem: Sulindac ve α-laktalbuminin etkileri 24, 48 ve 72. saatlerde MTT metodu ile değerlendirildi ve immünositokimyasal olarak apoptoz belirteçleri kaspaz3, kaspaz-9 ve sitokrom-c, ve TUNEL boyama yöntemleri uygulandı. Bulgular: Sulindac ve α-laktalbuminin hem primer hem de metastatik kolon karsinoma hücrelerinde apoptoz mekanizmalarını tetikleyebileceği ve primer kolon karsinom hücrelerinin, metastatik hücrelerden daha fazla etkilendiği saptandı. Sonuç: Sulindac ve α-laktalbuminin, gelecekte in vivo çalışmalarda ve kanser tedavilerinde kullanılabileceği düşünülmektedir.