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Journals / Türk Biyokimya Dergisi / 2019 / Cilt: 44 - Sayı: 3

Comparison of pendimethalin binding properties of serum albumins from various mammalian species

Çeşitli memeli türlerinden serum albüminlerinin pendimetaline bağlanma özelliklerinin karşılaştırılması

Nurul Iman Ahamed KAMEEL
Saad TAYYAB
Saharuddin MOHAMAD
Wei Qi LEE
Pages
363–369
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The PM binding affinity of these albuminswas found to follow the order: SSA > BSA > RbSA > PSA.Warfarin (WFN) displacement results also suggested similardisplacing action of PM on WFN-BSA complex, when comparedto the published results on WFN-HSA complex.Conclusion: The results suggested close similarity betweenBSA and HSA in terms of PM binding characteristics andhence bovine can be selected as a suitable animal modelfor further toxicological studies of PM.

📌 3 Key Takeaways

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Objective & Hypothesis:Background: To investigate the interaction of pendimethalin(PM), a commonly used herbicide, with various mammalianserum albumins.Methods: The interactions of PM with serum albuminsof bovine (BSA), sheep (SSA), porcine (PSA) and rabbit(RbSA) were studied using fluorescence quenching titrationand site marker displacement experiments.Results: A comparison of the PM-induced quenching ofthe fluorescence of these albumins with that published forhuman serum albumin (HSA) showed similarity betweenBSA and HSA.
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Key Findings & Data:Background: To investigate the interaction of pendimethalin(PM), a commonly used herbicide, with various mammalianserum albumins.Methods: The interactions of PM with serum albuminsof bovine (BSA), sheep (SSA), porcine (PSA) and rabbit(RbSA) were studied using fluorescence quenching titrationand site marker displacement experiments.Results: A comparison of the PM-induced quenching ofthe fluorescence of these albumins with that published forhuman serum albumin (HSA) showed similarity betweenBSA and HSA.
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Conclusion & Impact:The PM binding affinity of these albuminswas found to follow the order: SSA > BSA > RbSA > PSA.Warfarin (WFN) displacement results also suggested similardisplacing action of PM on WFN-BSA complex, when comparedto the published results on WFN-HSA complex.Conclusion: The results suggested close similarity betweenBSA and HSA in terms of PM binding characteristics andhence bovine can be selected as a suitable animal modelfor further toxicological studies of PM.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background: To investigate the interaction of pendimethalin(PM), a commonly used herbicide, with various mammalianserum albumins.Methods: The interactions of PM with serum albuminsof bovine (BSA), sheep (SSA), porcine (PSA) and rabbit(RbSA) were studied using fluorescence quenching titrationand site marker displacement experiments.Results: A comparison of the PM-induced quenching ofthe fluorescence of these albumins with that published forhuman serum albumin (HSA) showed similarity betweenBSA and HSA. The PM binding affinity of these albuminswas found to follow the order: SSA > BSA > RbSA > PSA.Warfarin (WFN) displacement results also suggested similardisplacing action of PM on WFN-BSA complex, when comparedto the published results on WFN-HSA complex.Conclusion: The results suggested close similarity betweenBSA and HSA in terms of PM binding characteristics andhence bovine can be selected as a suitable animal modelfor further toxicological studies of PM.

Özet

Amaç: Yaygın olarak kullanılan bir herbisit olan pendimethalin (PM) ile çeşitli memeli serum albüminlerinin etkileşimini araştırmak. Gereç ve Yöntem: PM’nin sığır (BSA), koyun (SSA), domuz (PSA) ve tavşan (RbSA) serum albüminleri ile etkileşimleri floresan söndürme titrasyonu ve yer işareti deplasman deneyleri kullanılarak incelenmiştir. Bulgular: Bu albüminlerin PM kaynaklı floresan söndürmesi ile insan serum albümin (HSA) için yayınlanmış değerlerin karşılaştırması, BSA ve HSA arasında benzerlik olduğunu gösterdi. Bu albüminlere PM bağlanma afinitesinin şu sırayı takip ettiği bulunmuştur: SSA > BSA > RbSA > PSA. Varfarin (WFN) deplasman sonuçları, WFN-HSA kompleksi üzerine yayınlanmış sonuçlarla karşılaştırıldığında, WFN-BSA kompleksi üzerinde PM’nin benzer yer değiştirme aktivitesini ortaya koymuştur. Sonuç: Sonuçlar, PM bağlanma özellikleri açısından BSA ve HSA arasında yakın benzerlik olduğunu ve dolayısıyla sığırın, PM’nin ileri toksikolojik çalışmaları için uygun bir hayvan modeli olarak seçilebileceğini göstermiştir.

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Cite This Article

KAMEEL, N. I. A., TAYYAB, S., MOHAMAD, S., LEE, W. Q. (2022). Comparison of pendimethalin binding properties of serum albumins from various mammalian species. Türk Biyokimya Dergisi, Cilt: 44 - Sayı: 3, 363–369. https://ajindex.com/articles/620871

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