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Journals / Türk Biyokimya Dergisi / 2019 / Cilt: 44 - Sayı: 4

Antioxidant and cytotoxic activity of three Turkish marine-derived fungi

Türkiye denizlerinden elde edilen üç mantarın antioksidan ve sitotoksik aktiviteleri

Bülent GÖZCELİOĞLU
Pages
554–559
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This serves asa valuable preliminary study for activity-guided isolationof secondary metabolites.

📌 3 Key Takeaways

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Objective & Hypothesis:Background: Marine-derived fungi are appraised as afavorable source for discovering new bioactive secondarymetabolites.
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Key Findings & Data:In the last few decades researchers haveconcentrated on marine-derived fungi to obtain new andpharmaceutically active bioactive secondary metaboliteswith therapeutic potential.Objective: In this study three marine-derived fungi were isolated and identified from marine invertebrates and investigated with regard to their antioxidant and cytotoxic activities.Materials and methods: DPPH, SO, NO, and ABTS assayswere used for monitoring free radical scavenging activity,and the MTT assay was used for testing cytotoxic activityagainst HCT-116 colon cancer cells.Results: According to the obtained results Malasseziarestricta extract was shown to have the highest antioxidant and cytotoxic activities compared to the other testedfungi strains.Conclusion: This study is the first report about the antioxidant and cytotoxicity activity of Acremonium sclerotigenum, Aspergillus flavus, and M. restricta.
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Conclusion & Impact:This serves asa valuable preliminary study for activity-guided isolationof secondary metabolites.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background: Marine-derived fungi are appraised as afavorable source for discovering new bioactive secondarymetabolites. In the last few decades researchers haveconcentrated on marine-derived fungi to obtain new andpharmaceutically active bioactive secondary metaboliteswith therapeutic potential.Objective: In this study three marine-derived fungi were isolated and identified from marine invertebrates and investigated with regard to their antioxidant and cytotoxic activities.Materials and methods: DPPH, SO, NO, and ABTS assayswere used for monitoring free radical scavenging activity,and the MTT assay was used for testing cytotoxic activityagainst HCT-116 colon cancer cells.Results: According to the obtained results Malasseziarestricta extract was shown to have the highest antioxidant and cytotoxic activities compared to the other testedfungi strains.Conclusion: This study is the first report about the antioxidant and cytotoxicity activity of Acremonium sclerotigenum, Aspergillus flavus, and M. restricta. This serves asa valuable preliminary study for activity-guided isolationof secondary metabolites.

Özet

Önbilgi: Deniz kaynaklı mantarlar, yeni biyoaktif sekonder metabolitlerin keşfedilmesi için uygun bir kaynak olarak değerlendirilmektedir. Son yıllarda, deniz kaynaklı mantarlar terapötik potansiyeli olan yeni biyoaktif sekonder metabolitlerin keşfi için araştırmacılar tarafından odak noktası haline gelmiştir. Amaç: Bu çalışmada 3 deniz kaynaklı mantar, Türkiye denizlerinde yaşayan omurgasızlarından izole edilmiş, türleri teşhis edildikten sonra antioksidan ve sitotoksik aktiviteleri açısından araştırılmıştır. Gereç ve Yöntemler: Deniz kaynaklı mantarların ekstrelerinin serbest radikal süpürücü aktivitelerini ölçmek için DPPH, SO, NO ve ABTS yöntemler ile ölçülmüştür, ve MTT yöntemi ile sitotoksik aktiviteleri, HCT 116 kolon kanseri hücrelerine karşı kullanılmıştır. Bulgular: Elde edilen sonuçlara göre, Malassezia restricta ekstresi test edilen diğer mantar suşları göre en yüksek antioksidan ve sitotoksik aktiviteler gösterdiği görülmüştür. Sonuç: Bu çalışma Acremonium sclerotigenum, Aspergillus flavus, Malassezia restricta’nın antioksidan ve sitotoksisite aktivitesi ile ilgili ilk rapordur. Bu çalışmanın önemli bir avantajı, sekonder metabolitlerin aktivite kılavuzlu izolasyonu için bir ön çalışmadır.

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Cite This Article

GÖZCELİOĞLU, B. (2022). Antioxidant and cytotoxic activity of three Turkish marine-derived fungi. Türk Biyokimya Dergisi, Cilt: 44 - Sayı: 4, 554–559. https://ajindex.com/articles/620868

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