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Journals / Türk Biyokimya Dergisi / 2019 / Cilt: 44 - Sayı: 5

The protective role of oleuropein against diethylnitrosamine and phenobarbital induced damage in rats

Sıçanlarda dietilnitrozamin ve fenobarbital ile oluşturulan hasara karşı oleuropeinin koruyucu rolü

Nalan KAYA
Gonca OZAN KOCAMÜFTÜOĞLU
Sema TEMİZER OZAN
Nurefşan ÖZEREN
Mehmet Ali KISACAM
Pages
714–721
Publication Date
2022-11-05
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

In the liver sections of the DEN,DEN + PB and OLE groups, increase in some histopathological findings and TUNEL positive cells were increased compared to the control group.Conclusion: OLE can be used as a protector against theeffects of carcinogens causing liver damage

📌 3 Key Takeaways

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Objective & Hypothesis:AbstractObjective: Liver cancer is amongst the most lethal cancers worldwide.
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Key Findings & Data:In this study, we aimed at investigating OLE against DEN + PB induced liver damage.Methods: Adult Sprague-Dawley rats were divided into 5 groups (n = 10): Control, DEN, DEN + PB, DEN + PB + OLE and OLE.
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Conclusion & Impact:In the liver sections of the DEN,DEN + PB and OLE groups, increase in some histopathological findings and TUNEL positive cells were increased compared to the control group.Conclusion: OLE can be used as a protector against theeffects of carcinogens causing liver damage
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

AbstractObjective: Liver cancer is amongst the most lethal cancers worldwide. Diethylnitrosamine (DEN) and phenobarbital (PB) are common agents that form reactive oxygen species (ROS). Oleuropein (OLE) has efficient biological propertiesand used as a therapeutic agent. In this study, we aimed at investigating OLE against DEN + PB induced liver damage.Methods: Adult Sprague-Dawley rats were divided into 5 groups (n = 10): Control, DEN, DEN + PB, DEN + PB + OLE and OLE. DEN, DEN + PB, DEN + PB + OLE groups were administered a single dose of 150 mg/kg DEN. After two weeks, DEN + PB and DEN + PB + OLE groups received 500 ppm of PB. 10 mg/kg/day of OLE was orally administered to DEN + PB + OLE and OLE groups. Biochemical and histopathological changes evaluated after the 8 weeks studyResults: DEN and PB application with OLE treatment resulted significant differences, alone or combined. Although there was a significant difference among thegroups in terms of liver GSH and MDA levels and CAT activities, there was no significant difference among the groups in SOD activity. In the liver sections of the DEN,DEN + PB and OLE groups, increase in some histopathological findings and TUNEL positive cells were increased compared to the control group.Conclusion: OLE can be used as a protector against theeffects of carcinogens causing liver damage

Özet

ÖzAmaç: Karaciğer kanseri dünya çapında görülen en ölümcül kanserlerdendir. Dietilnitrosamin (DEN) ve Fenobarbital (FB) reaktif oksijen türlerinin oluşumuna yol açan ajanlardır. Oleuropein (OLE) etkin biyolojik özellikleri olan ve tedavide kullanılan bir ajandır. Bu çalışmada DEN + FB ile indüklenen karaciğer hasarına karşı OLE’in etkisini araştırmak amaçlanmıştır.Yöntemler: Yetişkin Sprague-Dawley sıçanlar 5 gruba ayrıldı (n = 10): Kontrol, DEN, DEN + FB, DEN + FB + OLE, OLE. DEN, DEN + FB, DEN + FB + OLE gruplarına 150 mg/ kg tek doz DEN uygulaması yapıldı. İki hafta sonrasında DEN + FB ve DEN + FB + OLE gruplarının içme sularına 500 ppm FB konuldu. DEN + FB + OLE ve OLE gruplarına oral yolla 10 mg/kg/gün dozunda OLE uygulandı. Biyokimyasal ve histopatolojik değerlendirmeler 8 haftalık çalışma sonunda yapıldı.Bulgular: DEN ve FB uygulaması ile OLE tedavisi birlikte veya kombine uygulandığında belirgin değişimler gösterdi. Karaciğerde GSH, MDA düzeyleri ile CAT aktivitesi açısından anlamlı fark olmasına rağmen SOD aktivitesi açısından gruplar arasında istatiki farka rastlanamamıştır. DEN, DEN + FB, OLE gruplarının karaciğer kesitlerinde kontrol grubuna kıyasla bazı histopatolojik bulgularda ve TUNEL pozitif hücrelerde artışa rastlanmıştır.Sonuç: OLE’ in karaciğer hasarına sebep olabilen karsinojenlerin etkilerine karşı koruyucu olarak kullanılabileceği ortaya konmuştur.

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Cite This Article

KAYA, N., KOCAMÜFTÜOĞLU, G. O., OZAN, S. T., ÖZEREN, N., KISACAM, M. A. (2022). The protective role of oleuropein against diethylnitrosamine and phenobarbital induced damage in rats. Türk Biyokimya Dergisi, Cilt: 44 - Sayı: 5, 714–721. https://ajindex.com/articles/620826

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