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Journals / Türk Biyokimya Dergisi / 2020 / Cilt: 45 - Sayı: 3

Metformin suppresses the proliferationand invasion through NF-kB and MMPs in MCF-7cell line

Metformin MCF-7 hücre hattında NF-kB ve MMP’ler yoluyla proliferasyonu ve invazyonu baskılar

Seyhun SOLAKOĞLU
Sibel DOĞAN
Matem TUNÇDEMİR
Elif YAPRAK SARAÇ
Güven YENMİŞ
Gönül KANİGUR SULTUKBEY
Nail BEŞLİ
Pages
295–304
Publication Date
2022-11-06
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Ten millimolar metformin also reduced expression of MMP-9 significantly (p < 0.05).Conclusion: Metformin may act on the proliferation, and the processes of invasion and metastasis of MCF-7 cells through blocking NF-kB, which is intensely expressed in breast cancer cells, and through diminishing the expression of MMP-2 and MMP-9 significantly.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: MCF-7 cells, a breast cancer cell line, are used for experiments of estrogen receptor (ER)-positive breast cancer and many sub-clones representing different classes of ER-positive tumors.
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Key Findings & Data:We aimed to determine the efficacy of metformin, a potential anti-cancer agent, on the cell proliferation, and the expressions of NF-kB (p65), MMP-2 and MMP-9 in MCF-7 cell line.Materials and methods: MCF-7 cells (human breast adenocarcinoma) were treated with elevating doses of metformin (0–50 mM) for 24 h.
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Conclusion & Impact:Ten millimolar metformin also reduced expression of MMP-9 significantly (p < 0.05).Conclusion: Metformin may act on the proliferation, and the processes of invasion and metastasis of MCF-7 cells through blocking NF-kB, which is intensely expressed in breast cancer cells, and through diminishing the expression of MMP-2 and MMP-9 significantly.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: MCF-7 cells, a breast cancer cell line, are used for experiments of estrogen receptor (ER)-positive breast cancer and many sub-clones representing different classes of ER-positive tumors. We aimed to determine the efficacy of metformin, a potential anti-cancer agent, on the cell proliferation, and the expressions of NF-kB (p65), MMP-2 and MMP-9 in MCF-7 cell line.Materials and methods: MCF-7 cells (human breast adenocarcinoma) were treated with elevating doses of metformin (0–50 mM) for 24 h. The anti-proliferative effect of metformin was studied by BrdU proliferation assay, and the expression levels of NF-kB (p65), MMP-2 and MMP-9 were analyzed by immunocytochemical staining.Results: The percentage of cell proliferation was reduced significantly by 10 and 50 mM doses of metformin (p < 0.001). The expression levels of nuclear NF-kB (p65), MMP-9 and MMP-2 were considerably reduced in 50 mM metformin treated cells while the expression of cytoplasmic NF-kB (p65) elevated compared to control group (p < 0.05). Ten millimolar metformin also reduced expression of MMP-9 significantly (p < 0.05).Conclusion: Metformin may act on the proliferation, and the processes of invasion and metastasis of MCF-7 cells through blocking NF-kB, which is intensely expressed in breast cancer cells, and through diminishing the expression of MMP-2 and MMP-9 significantly.

Özet

Amaç: Bir meme kanseri hücre soyu olan MCF-7, östrojenreseptörü (ER) pozitif meme kanseri ve ER-pozitif tümör lerin farklı sınıflarını temsil eden birçok alt klonun deney leri için kullanılır. Potansiyel bir anti-kanser ajanı olanmetforminin, MCF-7 hücre soyunda hücre çoğalması veNF-kB (p65), MMP-2 ve MMP-9 ekspresyonları üzerindekietkisini belirlemeyi amaçladık. Gereç ve Yöntem: MCF-7 hücreleri (insan meme adeno karsinomu) 24 saat boyunca metformin artan dozları(0–50 mM) ile muamele edildi. Metforminin anti-proli feratif etkisi, BrdU proliferasyon deneyi ile incelendi veNF-kB (p65), MMP-2 ve MMP-9 ekspresyon seviyeleri iseimmünositokimyasal boyama ile analiz edildi. Bulgular: Hücre proliferasyonunun yüzdesi, 10 ve50 mM’lik metformin dozları ile önemli ölçüde azaldı(p < 0.001). Nükleer NF-kB (p65), MMP-9 ve MMP-2’ninekspresyon seviyeleri, 50 mM metformin ile muameleedilmiş hücrelerde önemli ölçüde azalırken, sitoplaz mik NF-kB’nin (p65) ekspresyonu, kontrol grubuna göre(p < 0.05) arttı. 10 mM metformin de MMP-9 ekspresyo nunu anlamlı şekilde azalttı (p < 0.05). Sonuç: Metformin, meme kanseri hücrelerinde yük sek oranda eksprese edilen NF-kB(p65) ve MMP-2 veMMP-9 ekspresyon seviyelerini önemli ölçüde azaltarak,MCF-7 hücrelerinin proliferasyonu, invazyonu ve metas tazı üzerinde etkili olabilir.

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Cite This Article

SOLAKOĞLU, S., DOĞAN, S., TUNÇDEMİR, M., SARAÇ, E. Y., YENMİŞ, G., SULTUKBEY, G. K., BEŞLİ, N. (2022). Metformin suppresses the proliferationand invasion through NF-kB and MMPs in MCF-7cell line. Türk Biyokimya Dergisi, Cilt: 45 - Sayı: 3, 295–304. https://ajindex.com/articles/620800

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